Efficacy of the nematode-trapping fungus Duddingtonia flagrans against three species of gastro-intestinal nematodes in laboratory faecal cultures from sheep and goats

Waghorn, T.S.; Leathwick, Dave M.; Chen, L.-Y; Skipp, R. A.

doi:10.1016/j.vetpar.2003.09.018

Cited by 47 publications

(34 citation statements)

References 13 publications

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“…The overall reductions (52 to 98%) in the larval development of gastro-intestinal nematodes obtained in the present study with dose rates ranging from 1.25 × 10 5 to 5 × 10 5 chlamydospores/kg BW/day are in accordance with the results of previous studies in goats [17,18,24]. Paraud and Chartier [17] and Paraud et al [18] reported reductions higher than 80% of the larval development of Teladorsagia circumcincta or Trichostrongylus colubriformis after the administration of 5 × 10 5 chlamydospores/ kg BW/day when compared to the control.…”

Section: Discussionsupporting

confidence: 92%

“…Moreover, at the same dose rate (2.5 × 10 5 chlamydospores/kg BW/day) in two consecutive experiments, these authors found a larval reduction ranging from 54.8 to 80.2%. On the contrary, Waghorn et al [24] reported a dose response effect in sheep and goats [15]. Thus the estimate of mean eggs per gram may be, to some extent, different from the true value that is later involved in coproculture.…”

Section: Discussionmentioning

confidence: 95%

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Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials

et al. 2005

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-The ability of the nematophagous fungus Duddingtonia flagrans to reduce the number of infective nematode larvae in coproculture was investigated in goats using different doses of chlamydospores (0, 1.25 × 10 5 , 2.5 × 10 5 , 5 × 10 5 chlamydospores/kg BW/day) given by oral administration or by voluntary consumption in feed during natural or experimental infections with nematodes. The kinetics of excretion of D. flagrans chlamydospores in the faeces was also determined using a dose of 5 × 10 5 chlamydospores/kg BW/day for five days. For all the trials, the faecal nematode egg outputs were determined by a modified McMaster method and standard coprocultures were set up (14 days, 25 °C) to determine the number of larvae emerging from culture in fungus treated and control faeces. When chlamydospores were orally administered, the number of larvae were reduced by 50 to 97% when compared to control cultures. No difference in the level of larval emergence from the culture was seen for experimental or natural infections at the different chlamydospore dose rates. In contrast, when chlamydospores were distributed in the feed, a dosedependent relationship was observed 10 days after the start of administration, the larval development being 2.0%, 14.0% and 86.9% for 5 × 10 5 , 2.5 × 10 5 and 0 spores/kg BW/day, respectively. In addition, the kinetic study showed that the larval emergence from coproculture in the fungus group was statistically lower than in the control group from the second day of administration of the chlamydospores and remained lower until the second day after the last administration (p < 0.05). The results indicate that, for goats in farm conditions, a minimum daily dose of 5 × 10 5 chlamydospores/kg BW must be used to ensure a high treatment efficacy and that daily administration is preferable for maintenance of efficacy over time.goat / biological control / Duddingtonia flagrans / nematode parasite / dose trials

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials

et al. 2005

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“…Challenges to fungal control have been a requirement for daily administering of fungi to the host and achieving the required fungal density inside the dung. However, a nematode-killing fungus, Duddingtonia flagrans, recently discovered in New Zealand (Skipp et al, 2002), was shown to have a trapping efficiency rate of 78% and activity for up to 90 days on pasture, providing a viable alternative to reduce animal mortality from nematode infections (Waghorn et al, 2003).…”

Section: Pasture Management Biological Control and Nutrient Supplemementioning

confidence: 99%

Parasitic nematodes—From genomes to control

Mitreva

Zarlenga

McCarter

et al. 2007

Veterinary Parasitology

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The diseases caused by parasitic nematodes in domestic and companion animals are major factors that decrease production and quality of the agricultural products. Methods available for the control of the parasitic nematode infections are mainly based on chemical treatment, non-chemical management practices, immune modulation and biological control. However, even with integrated pest management that frequently combines these approaches, the effective and long-lasting control strategies are hampered by the persistent exposure of host animals to environmental stages of parasites, the incomplete protective response of the host and acquisition of anthelmintic resistance by an increasing number of parasitic nematodes. Therefore, the challenges to improve control of parasitic nematode infections are multi-fold and no single category of information will meet them all. However, new information, such as nematode genomics, functional genomics and proteomics, can strengthen basic and applied biological research aimed to develop improvements.In this review we will, summarize existing control strategies of nematode infections and discuss ongoing developments in nematode genomics. Genomics approaches offer a growing and fundamental base of information, which when coupled with downstream functional genomics and proteomics can accelerate progress towards developing more efficient and sustainable control programs. #

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“…flagrans is considered the most promising species for biological control of animal endoparasites (SAHOO & KHAN, 2016). From the beginning of the 90's until today, researchers have reported its effectiveness in the control of immature stages of parasites of cattle (LARSEN et al, 1995;SILVA et al, 2013), sheep, and goats (LARSEN et al, 1998;WAGHORN et al, 2003;OJEDA-ROBERTOS et al, 2008;OJEDA-ROBERTOS et al, 2015;FITZ-ARANDA et al, 2015). Although this fungus is one of the most studied organism for parasite control, data from in vitro experiments based in vivo calculations, describing their nematode predation, are restricted.…”

Section: Introductionmentioning

confidence: 99%

In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations

Molento

Araújo

Buzatti

et al. 2017

Rev. Bras. Parasitol. Vet.

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Duddingtonia flagrans has been tested as an alternative parasite control, but data from in vitro experiments based on in vivo calculations describing nematophagous fungi predation in nematodes are restricted. The objective of this work was to determine the efficacy of D. flagrans against sheep nematode larvae in vitro using in vivo calculations. Fecal samples were introduced to fungi in different concentrations: 0.0/control; 0.05; 0.1; 0.2; 0.4; 0.8; 1.6; 3.2; and 6.4 g corresponding, respectively, to 583.000; 1.166.000; 2.332.000; 4.664.000; 9.328.000; 18.656.000; 37.312.000 and 74.624.000 chlamydospores/kg of body weight. The material was incubated for 14 days, before the larvae recovery (Assay 1). Assay 2 was carried out with the doses of 0.00625; 0.0125; and 0.025 g. The results showed a negative correlation between fungal concentrations and larval numbers for both assays. The fungus demonstrated an efficacy above 89% in both assays. Thus, we consider that the data from in vitro studies based on in vivo calculations may optimize the fungi quantities for field experiments.Keywords: Duddingtonia flagrans, predatory action, larvae, sheep, gastrointestinal nematodes. ResumoDuddingtonia flagrans tem sido testado como uma alternativa no controle de parasitos, entretanto, trabalhos in vitro da predação de nematoides por fungos nematófagos correlacionados com cálculos baseados para testes in vivo são restritos. O objetivo deste trabalho foi determinar a eficácia in vitro de D. flagrans contra larvas de nematoides de ovinos tendo como base cálculos in vivo. Amostras fecais receberam a adição do fungo em diferentes concentrações: 0.0/controle; 0,05; 0,1; 0,2; 0,4; 0,8; 1,6; 3,2 e 6,4 gramas correspondendo, respectivamente, às seguintes dosagens: 583.000; 1.166.000; 2.332.000; 4.664.000; 9.328.000; 18.656.000; 37.312.000 e 74.624.000 clamidósporos/Kg de peso vivo animal. O material foi incubado por 14 dias, para recuperação das larvas (Ensaio 1). O Ensaio 2 foi realizado com concentrações de 0,00625; 0,0125 e 0,025 g. Foi observada correlação negativa entre a concentração fúngica e o número de larvas, nos dois ensaios. O fungo demonstrou eficácia acima de 89% em ambos os ensaios. A partir destes dados, acreditamos que ensaios in vitro baseados em cálculos in vivo podem aprimorar as dosagens para a realização de experimentos a campo.

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Efficacy of the nematode-trapping fungus Duddingtonia flagrans against three species of gastro-intestinal nematodes in laboratory faecal cultures from sheep and goats

Cited by 47 publications

References 13 publications

Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials

Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials

Parasitic nematodes—From genomes to control

In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations

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