Parasitic nematodes—From genomes to control

Mitreva, Makedonka; Zarlenga, Dante S.; McCarter, James P.; Jasmer, Douglas P.

doi:10.1016/j.vetpar.2007.05.008

Cited by 43 publications

(30 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Different aspects in host-parasite relationships have benefited from genomic approaches in other parasitic nematodes including the entomopathogenic nematode Heterorhabditis bacteriophora [18–20], however, very little is known about the genomics of S. carpocapsae . In this work we constructed a cDNA library with transcripts from the parasitic phase of the nematode and we sequenced and analyzed 2500 ESTs that resulted in the identification of a set of genes that are putatively involved in parasitic mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Transcripts analysis of the entomopathogenic nematode Steinernema carpocapsae induced in vitro with insect haemolymph

Hao

Montiel

Abubucker

et al. 2010

Molecular and Biochemical Parasitology

Self Cite

View full text Add to dashboard Cite

Steinernema carpocapsae is an insect parasitic nematode widely used in pest control programs. The efficacy of this nematode in controlling insects has been found to be related to the pathogenicity of the infective stage. In order to study the parasitic mechanisms exhibited by this parasite, a cDNA library of the induced S. carpocapsae parasitic phase was generated. A total of 2500 clones were sequenced and 2180 high-quality ESTs were obtained from this library. Cluster analysis generated a total of 1592 unique sequences including 1393 singletons. About 63% of the unique sequences had significant hits (e≤1e-05) to the non-redundant protein database. The remaining sequences most likely represent putative novel protein coding genes. Comparative analysis identified 377 homologs in C. elegans, 431 in C. briggsae and 75 in other nematodes. Classification of the predicted proteins revealed involvement in diverse cellular, metabolic and extracellular functions. One hundred and nineteen clusters were predicted to encode putative secreted proteins such as proteases, proteases inhibitors, lectins, saposin-like proteins, acetylcholinesterase, anti-oxidants, and heat-shock proteins, which can possibly have host interactions. This dataset provides a basis for genomic studies towards a better understanding of the events that occur in the parasitic process of this entomopathogenic nematode, including invasion of the insect haemocoelium, adaptations to insect innate immunity and stress responses, and production of virulence factors. The identification of key genes in the parasitic process provides useful tools for the improvement of S. carpocapsae as a biological agent.

show abstract

Section: Introductionmentioning

confidence: 99%

Transcripts analysis of the entomopathogenic nematode Steinernema carpocapsae induced in vitro with insect haemolymph

Hao

Montiel

Abubucker

et al. 2010

Molecular and Biochemical Parasitology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Si comparamos esta situación con la de los nematodos parásitos, solamente nueve de ellos han sido completamente secuenciados (76) . C. elegans va muy adelantado puesto que también su transcriptoma está completamente descrito y disponible (www.nematodes.org).…”

Section: Elegans Como Modelo Para Estudios De Actividad Antihelmínunclassified

El nematodo Caenorhabditis elegans como modelo para evaluar el potencial antihelmíntico de extractos de plantas

Peña

Vazquez

Sánchez

et al. 2017

Rev. Mex. Cienc. Pecu.

View full text Add to dashboard Cite

RESUMENLos nematodos parásitos afectan la productividad pecuaria, dando lugar a pérdidas económicas considerables. Sin embargo, son pocos los antihelmínticos disponibles en el mercado y la resistencia de los parásitos a los existentes va en incremento, por lo que su control es actualmente ineficiente. En este sentido, la búsqueda de nuevas estrategias para controlar la parasitosis es una necesidad actual. Una posibilidad para el control estriba en la investigación de metabolitos derivados de plantas, ya que el conocimiento sobre su uso tradicional es un referente de inicio. Sin embargo, la eficacia antihelmíntica, así como los mecanismos de acción de plantas con uso tradicional como antiparasitarios, poco se han investigado científicamente, y en la mayoría de los casos se desconocen los compuestos activos responsables del efecto anti-parasitario. Una de las limitantes del estudio experimental del potencial antihelmíntico es la dificultad de trabajar con modelos parásitos. En este sentido, el nematodo de vida libre C. elegans es un modelo útil para entender el mecanismo de acción de drogas antihelmínticas. Por un lado, este nematodo se puede mantener y manipular en laboratorio con facilidad; por otro, comparte características fisiológicas y homología genética con nematodos parásitos, lo que permite comparaciones válidas. En esta revisión se pretende mostrar el potencial de la búsqueda de compuestos activos derivados de plantas tradicionalmente utilizadas como antiparasitarios como punto de partida en el desarrollo de nuevos fármacos antihelmínticos, así como resaltar las ventajas que C. elegans proporciona para entender los mecanismos de acción de nuevos compuestos. PALABRAS CLAVE: Resistencia

show abstract

“…For this release of Nematode.net, we have added gene annotations for Trichinella spiralis (17). As the PNGP (18,19) progresses and new genome resources are made available, we plan to add them into the NemaBrowse, along with Rfam predictions, NemaSNP variants and gene-calling evidence as analysis becomes available. The end goal would be to include the final assembly and annotation in WormBase (11) as it is a resource that has already started hosting non- Caenorhabditis species, however the completion of a genome project is a lengthy process (even when next-generation sequence data is used), therefore an immediate release of the interim products enables use of the data by the research community as soon as they are generated.…”

Section: Data-mining Portals: Updates and Improvementsmentioning

confidence: 99%

Nematode.net update 2011: addition of data sets and tools featuring next-generation sequencing data

Martin¹,

Abubucker²,

Heizer³

et al. 2011

Nucleic Acids Research

View full text Add to dashboard Cite

Nematode.net (http://nematode.net) has been a publicly available resource for studying nematodes for over a decade. In the past 3 years, we reorganized Nematode.net to provide more user-friendly navigation through the site, a necessity due to the explosion of data from next-generation sequencing platforms. Organism-centric portals containing dynamically generated data are available for over 56 different nematode species. Next-generation data has been added to the various data-mining portals hosted, including NemaBLAST and NemaBrowse. The NemaPath metabolic pathway viewer builds associations using KOs, rather than ECs to provide more accurate and fine-grained descriptions of proteins. Two new features for data analysis and comparative genomics have been added to the site. NemaSNP enables the user to perform population genetics studies in various nematode populations using next-generation sequencing data. HelmCoP (Helminth Control and Prevention) as an independent component of Nematode.net provides an integrated resource for storage, annotation and comparative genomics of helminth genomes to aid in learning more about nematode genomes, as well as drug, pesticide, vaccine and drug target discovery. With this update, Nematode.net will continue to realize its original goal to disseminate diverse bioinformatic data sets and provide analysis tools to the broad scientific community in a useful and user-friendly manner.

show abstract

Parasitic nematodes—From genomes to control

Cited by 43 publications

References 109 publications

Transcripts analysis of the entomopathogenic nematode Steinernema carpocapsae induced in vitro with insect haemolymph

Transcripts analysis of the entomopathogenic nematode Steinernema carpocapsae induced in vitro with insect haemolymph

El nematodo Caenorhabditis elegans como modelo para evaluar el potencial antihelmíntico de extractos de plantas

Nematode.net update 2011: addition of data sets and tools featuring next-generation sequencing data

Contact Info

Product

Resources

About