Efficacy of the ingredient A97614A1 in a model of reconstructed human epidermis stressed by cytokines

Ollivier, Elodie; Zemirline, Claudine; Amalric, Nicolas; Rahoul, Valérie; Reymond, Nadège; Cadiergues, Marie‐Christine

doi:10.22233/9781910443699.62.2

Cited by 4 publications

(6 citation statements)

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“…Confidence interval at 95% was at this time point [−85.7; −57.0]. This reduction can be linked to the antiseptic effect of chlorhexidine digluconate at 3% but also to soothing properties of Ophytrium, which has been shown to decrease the production of pro-inflammatory cytokines in vitro (15).…”

Section: Discussionmentioning

confidence: 84%

“…In this study, 100% of owners stated that they thought the skin of their pet was hydrated where the product was applied, indicating that the formula allowed the skin to be hydrated. This hydration may be partly due to the inclusion in the formula of Ophytrium which has been shown to limit water loss in a model of reconstructed epidermis stressed by pro-inflammatory cytokines (15).…”

Section: Discussionmentioning

confidence: 99%

“…Pads or wipes impregnated with antiseptic ingredients offer an interesting two-fold action: a mechanical one to remove microbes, crusts, dead cells, and a microbiological one by bringing the antiseptic ingredient (chlorhexidine in our case) directly to the skin. The additional benefit of the pads tested in the study is to help strengthen the mechanical, immunological, and microbiological skin barriers thanks to the three-fold action of Ophytrium contained in the formula (15,16).…”

Section: Discussionmentioning

confidence: 99%

“…It was shown in vitro to have an action on the mechanical, microbiological, and immunological skin barriers. It increased tight junctions, filaggrin, Natural Moisturizing Factors contents, ceramides, and limited Trans Epidermal Water Loss to strengthen the mechanical skin barrier (15). It limited the adhesion and biofilm formation of S. aureus and S. pseudintermedius (16) to restore the balance of the protective microbial flora.…”

Section: Introductionmentioning

confidence: 99%

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Performance of Daily Pads Containing Ophytrium and Chlorhexidine Digluconate 3% in Dogs With Local Cutaneous Bacterial and/or Malassezia Overgrowth

Gatellet

Kesteman

Baulez³

et al. 2021

Front. Vet. Sci.

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Local disturbances of the microbiota are common in dogs with underlying skin conditions. Antiseptic topical products are indicated to control such superficial disorders. The objective of this study was to evaluate the performance of a daily application of pads containing Ophytrium and chlorhexidine digluconate 3% (DOUXO® S3 PYO Pads, Ceva Santé Animale, France) in dogs with focal bacterial and/or Malassezia overgrowth. Eighteen dogs with focal skin dysbiosis were included in the analysis of this prospective, multicentric, field study. Dogs received daily pad applications for 14 days. Bacterial and/or Malassezia counts per microscopic field and a global score of the most affected patch (0–17 scale based on extension, severity, bacterial, and Malassezia cytological scores) were assessed by a veterinarian and pruritus by the owner (Pruritus Visual Analog Scale) on days (D)0, D7, D14. Owner and veterinarian evaluations for performance and satisfaction were recorded. Eleven dogs had primarily cocci overgrowth and seven mostly Malassezia. Mean bacterial and Malassezia counts decreased after 14 days (6.9–1.1; 7.6–1.5, respectively); 88.9% of dogs achieved a ≥70% microbial decrease and had ≤2 bacteria and ≤1 Malassezia per oil field. Mean global score of the most affected patch and pruritus score significantly improved at D14, respectively, from 8.6 to 2.6 and 4.5 to 1.2 (P < 0.05 each, mean improvements of 70.4 and 71.4%, respectively). Global veterinary assessment of the protocol was satisfactory, good, or excellent in 88.9% of cases. Most owners (94.4%) considered the protocol efficacious. Using a pad containing Ophytrium and chlorhexidine digluconate 3% daily for 14 days improved the skin condition and pruritus of dogs with local dysbiosis, resulting in high satisfaction levels for both veterinarians and dog owners.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Performance of Daily Pads Containing Ophytrium and Chlorhexidine Digluconate 3% in Dogs With Local Cutaneous Bacterial and/or Malassezia Overgrowth

Gatellet

Kesteman

Baulez³

et al. 2021

Front. Vet. Sci.

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“…O. japonicus is known to be an effective anti-inflammatory herbal medicine in many Asian countries [ 11 ]. Ophytrium has been assessed in vitro in reconstructed epidermis models [ 12 , 13 ]. It limited the adhesion and biofilm formation of Staphylococcus pseudintermedius at the surface of the reconstructed canine epidermis [ 13 ], and was shown in vitro to have beneficial effects on the mechanical and immunological skin barriers [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Performance and Tolerance of a Protocol for Idiopathic Chronic Greasy Seborrhea in 18 Dogs Using a Shampoo and Mousse Containing Plant Extracts

Kondratjeva

Brun

Amalric³

et al. 2023

Veterinary Sciences

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The study aimed to evaluate the tolerance, performance and effect on hair lipids and skin hydration of a protocol combining applications of one shampoo and subsequent mousses containing plant extracts (Ophytrium and Seboliance) in dogs with an undiagnosed chronic greasy keratinisation disorder. Six dogs were washed with plain water on day (D)0. Twelve dogs were shampooed on D0 and received eight mousse applications at 48–72 h intervals from D2 to D18. Clinical score (CS), Natural Moisturizing Factors (NMF) and hair lipids (HL) were evaluated on D0, D0 + 4 h, D7, D14 and D24. At baseline, no significant differences were observed in CS, NMF and HL between groups. In the control group, CS and HL remained stable throughout the study while a slight decrease in NMF was observed at D0 + 4 h. CS was significantly reduced in the test group between D0 and D7 (−53%) which reached 91% at D24 (p < 0.05), with no side effects. NMF levels decreased in the test group at D0 + 4 h (−73%, p < 0.0001) and returned to baseline from D14. In conclusion, one shampoo and subsequent mousse applications rapidly and safely improved coat quality in dogs with an undiagnosed keratinisation disorder without affecting NMF and HL contents over the study period.

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In vitro efficacy of a topical antimicrobial mousse and shampoo at proximal and distal hair locations against Staphylococcus pseudintermedius and Staphylococcus schleiferi

Taketa,

Rosenkrantz,

Griffin

et al. 2023

Veterinary Dermatology

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BackgroundTopical therapy is essential in assisting with the resolution of pyoderma.Objectives(i) Evaluate the in vitro efficacy and residual activity of two different hair segments treated with shampoo and mousse against meticillin‐sensitive and meticillin‐resistant staphylococci; (ii) compare proximal and distal hair portions treated with the products and (iii) describe a new disc diffusion method for assessing residual efficacy.AnimalsEleven privately owned, medium‐haired dogs.Materials and MethodsIn this randomised, blinded and negatively controlled study, dogs were treated once with a 3% chlorhexidine digluconate‐0.5% ophytrium shampoo on the lateral thorax, and the corresponding mousse on the opposite side. Hairs were plucked before treatment, two hours post‐treatment, and day (D)2, D4, D7, D10 and D14. Hairs were weighed (0.01 g) and cut (1.0 cm) from the proximal portion, moistened with saline and placed on a sterile diffusion disc to absorb the solution. Proximal and distal hair bundles and diffusion discs were placed onto agar inoculated with an isolate of meticillin‐sensitive or meticillin‐resistant Staphylococcus pseudintermedius or Staphylococcus schleiferi. Inhibition zones were measured following incubation.ResultsDistal hairs had larger (p < 0.001) inhibition zones compared to proximal hairs. Mousse had significant differences (p < 0.05) between time points and locations for both the hair bundles and discs, while shampoo only had a significant difference (p < 0.001) between time points for the hairs.Conclusions and Clinical RelevanceMousse was effective, and shampoo was only minimally effective in inhibiting bacterial growth in vitro, with the greatest effect occurring at the two hours time point. The distal hair shafts had greater inhibition.

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Efficacy of the ingredient A97614A1 in a model of reconstructed human epidermis stressed by cytokines

Cited by 4 publications

References 0 publications

Performance of Daily Pads Containing Ophytrium and Chlorhexidine Digluconate 3% in Dogs With Local Cutaneous Bacterial and/or Malassezia Overgrowth

Performance of Daily Pads Containing Ophytrium and Chlorhexidine Digluconate 3% in Dogs With Local Cutaneous Bacterial and/or Malassezia Overgrowth

Performance and Tolerance of a Protocol for Idiopathic Chronic Greasy Seborrhea in 18 Dogs Using a Shampoo and Mousse Containing Plant Extracts

In vitro efficacy of a topical antimicrobial mousse and shampoo at proximal and distal hair locations against Staphylococcus pseudintermedius and Staphylococcus schleiferi

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