Efficacy of sodium metabisulphite for control of Aspergillus flavus and aflatoxin B1 contamination in vitro and in chilli powder and whole red chillies

Aljaza, Diyaa; Medina, Ángel; Magan, Naresh

doi:10.1016/j.foodcont.2021.108786

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Sodium metabisulfite has previously been shown to have antifungal (e.g., Aspergillus spp., Fusarium spp., Rhizoctonia solani ) and antibacterial (e.g., Staphylococcus spp., Escherichia coli , Pseudomonas aeruginosa , Bacillus spp.) capabilities [ 32 , 33 , 34 , 35 , 36 ]. The broad-spectrum antimicrobial activity of sodium metabisulfite and its use in eye care solutions warranted further investigation into its activity against Acanthamoeba species.…”

Section: Discussionmentioning

confidence: 99%

Sodium Metabisulfite Inhibits Acanthamoeba Trophozoite Growth through Thiamine Depletion

Mooney,

Giammarini,

Corbett

et al. 2024

Pathogens

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Acanthamoeba keratitis (AK) is a severe infection of the cornea. Prevention and treatment are difficult due to the inefficacy of currently available compounds. The impact of many commonly used compounds for routine examinations of Acanthamoeba is unexplored but might offer insight useful in combatting AK. In this study, we demonstrate that sodium metabisulfite, a common preservation constituent of eye care solutions, was found to be active against Acanthamoeba trophozoites at concentrations lower than that commonly found in eye drops (IC50 0.03 mg/mL). We demonstrate that sodium metabisulfite depletes thiamine from growth medium and that Acanthamoeba is a thiamine auxotroph, requiring thiamine salvage for growth. The inhibitory effects of sodium metabisulfite can be overcome by thiamine supplementation. These results are consistent with the lack of key enzymes for thiamine biosynthesis in the genome of Acanthamoeba, an area which might prove exploitable using new or existing compounds. Indeed, this study highlights sodium metabisulfite as a useful inhibitor of Acanthamoeba castellanii trophozoites in vitro and that it acts, at least in part, by limiting available thiamine.

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