In newborns, Candida is responsible for the common oral thrush and rash in skin folds and in the diaper area. Before the advent of modern sanitary measures and topical antifungal treatments, infants died from dehydration due to severe oral mucocandidiasis. Oral thrush is more likely to occur in infants and older adults due to reduced immunity. The study aimed to shed light on the relationship between Thrush and Candidia and To molecular identification of Candidia spp. isolated from Thrush. There are 100 samples of Oral swabs collected from pediatric with Thrush at ages ranging from (1day-12 years). All samples cultured on SDA, MSA and MEA. Several tests were used to identify the types of isolated yeasts, including growth on Chromogenic agar medium, as well as biochemical and molecular tests using primers ITS1, ITS4. The Results found that a six species of Candida were isolated on Chromogenic: C. albicans, C. glabrata, C. tropicalis, C. dubliniensis, C. krusei and C. kefyr, Also, showed the number of yeasts that were genetically diagnosed by molecular diagnosis was five species of yeasts as follows 2 isolates C. tropicalis from 22% and one isolate for each of the following types C. albicans, C. kefyr, C. dubliniensis and C. spp with 11% of all isolates where C. albicans showed the highest percentage. The results of the statistical analysis of the isolates showed that the most affected age group of pediatric was (month-1 year) in the rate of 24%. Antifungal susceptibility was studied, as the results indicated that all Candida isolates were sensitive to itraconazole, while all Candida isolates were resistant to fluconazole Furthermore, most types of Candida were resistant to Nystatin and Amphotericin B, except for three type’s C. krusei, C. glabrata, and C. kefyr. The most common pathogen in pediatric is Candida spp. In pediatric, the results indicated that the best treatment to eliminate Candida Itraconazol, Clotrimazol, Nystatin, and Amphotricine B Nystatin respectively
Candida species are medically significant because they are the most frequent opportunistic mycosis in the worldwide. Because of hormonal and biochemical fluctuations, Fungal infections in the vagina are tenfold during pregnancy. Increased antibiotic usage and the presence of high levels of reproductive hormones during pregnancy both promote Candida species colonization. The study aimed to shed light on the relationship between Urinary tract infection UTI and candidiasis and determines the genetic patterns and their prevalence among women. There are 90 samples of urine and vaginal swabs collected from pregnant women with UTI at ages ranging from (15-40) years. All samples cultured on SDA, MSA and MEA. Several tests were used to identify the types of isolated yeasts, including growth on Chromogenic agar medium, as well as biochemical and molecular tests using primers ITS1, 4. The Results found that a nine species of Candida were isolated on Chromogenic: C. albicans, C. glabrata, C. tropicalis, C. dubliniensis, C. parapsilosis, C. krusei, C. kefyr, C. membranifaciens and C. utilis, Also, showed the number of yeasts that were genetically diagnosed by molecular diagnosis was eight species of yeasts as follows 3 isolates C. albicans from 33% and one isolate for each of the following types C. orthopsilosis, C. glabrata, C. tropicalis, C. dubliniensis and C. parapsilosis with 11% of all isolates where C. albicans showed the highest percentage. The results of the statistical analysis of the isolates showed that the most affected age group of pregnant women was (26-30), (21-25 year) in the rate of 33%, 30% respectively. Antifungal susceptibility was studied, as the results indicated that all Candida isolates were sensitive to Miconazole and itraconazole, except for Candida albicans, which was resistant to itraconazole. Furthermore, most types of Candida Were resistant to Nystatin and Amphotericin B, except for three type’s C. krusei, C. tropicalis, and C. utilis. The most common pathogen in Urinary tract system is Candida spp. in pregnant women, the results indicated that the best treatment to eliminate Candida Miconazole,Itraconazol, Clotrimazol, Co-Trimoxazol, Ketoconazole, Fluconazole, Amphotricine B , and Nystatin respectively.
Food spoilage and their contamination with mycotoxins are a significant issue for the food industry, leading to economic losses and a negative impact on public health all over the world. The objective of this study was to examine the preventive effect of different concentrations of Propylparaben (PP) for control of fungal populations and aflatoxin b1 (AFB1) contamination of stored chilli powder in both artificially and naturally contaminations. These treatments were examined at two different water activity (aw) levels (0.90 and 0.95 aw) in stored chilli powder at 30oC for 20 days. The total populations of A. flavus isolated from both artificially and naturally contamination of stored chilli powder at 30oC were significantly reduced by using PP treatments especially, with 2000 ppm. In additions, the AFB1 production was reduced when increased PP concentration compared to the untreated control. In conclusion, the economic and health impacts related to Aspergillus and AFB1 contamination could be minimised by adding PP as a food-grade preservative to stored chilli powder, Results show From a human health perspective, the use of PP is allowed as a food preservative by the (IARC) and (WHO). It must use according to legislation doses (0.1%) introduced by the law of (GRAS) regulations.
Chillies and chilli-based products are important spices on a global basis. The production, processing, transport and storage phases of chillies are prone to infection by Aspergillus Section Flavi and contamination with aflatoxins (AFs), especially aflatoxin B1 (AFB1) for which legislative limits exist in many countries. We have examined the effect of the interacting abiotic factors of water availability (water activity, aw; 0.995-0.850 aw) and temperature (15-37 °C) on (a) lag phases prior to growth, (b) growth, (c) AFB1 production and (d) contour maps of optimum and boundary conditions for colonisation and toxin production by three Aspergillus flavus strains on a 10% chilli-based medium. Additional studies with whole red chillies + A. flavus conidial inoculum on AFB1 contamination during storage for 10-20 days at 30 °C were also carried out. In vitro, the lag phases before growth were delayed by lower temperatures (15, 20 °C) and aw levels (0.928-0.901 aw). There was no statistical difference in growth between the three strains. Optimal growth was at 37 °C and 0.982 aw with no growth at 0.85 aw. Optimal temperature × aw conditions for AFB1 production were at 30 °C and 0.982 aw with no statistical difference in production between strains. No AFB1 was produced at 15-20 °C at 0.901 and 0.928 aw levels, respectively. In situ studies with A. flavus inoculated whole red chillies at 0.90 and 0.95 aw found that this species became the major component of the total fungal populations at 30 °C after 10-20 days storage. AFB1 contamination was above the European legislative limits (5 μg/kg) for spices at 0.90 aw after 20 days storage and at 0.95 aw after 10 and 20 days. This suggests that storage conditions of ≥0.90 aw, especially at ≥25-30 °C represents a significant risk of contamination with AFB1 at levels where rejection might occur, even after only 10-20 days storage.
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