Efficacy of sodium hypochlorite and peracetic acid against Aspergillus nomius in Brazil nuts

Ribeiro, Manuela; Freitas-Silva, Otniel; Castro, I. M. de; Teixeira, Alessandra da Silva; Silva, Sílvia Helena Marques da; Sales-Moraes, A.C.S.; Abreu, Lucimar Santiago de; Sousa, C. L.

doi:10.1016/j.fm.2020.103449

Cited by 15 publications

(12 citation statements)

References 25 publications

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“…Elemol compound, on the other side, has also shown some high biological activity [ 41 ]. The active ingredient pinene exhibited significant antibacterial capacity against P. aeruginosa and other multidrug-resistant bacteria [ 42 , 43 ]. More particularly, it also had strong antibacterial activity against S. aureus and E. coli strains [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

GC-MS Profiling, In Vitro Antioxidant, Antimicrobial, and In Silico NADPH Oxidase Inhibition Studies of Essential Oil of Juniperus thurifera Bark

Lafraxo

Moussaoui

Jardan

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Juniperus thurifera is a native species to the mountains of the western Mediterranean region. It is used in traditional medicine as a natural treatment against infections. The present study aimed to carry out the chemical analysis and evaluate the antioxidant, antimicrobial, as well as in silico inhibition studies of the essential oils from Juniperus thurifera bark (EOEJT). Chemical characterization of EOEJT was done by gas chromatography (GC-MS). We have performed three antioxidant assays (Reducing power (FRAP), 2, 2-diphenylpicrylhydrazyl (DPPH), and total antioxidant capacity (TAC)) of the EOEJT. We next evaluated the antimicrobial activity against in silico study, which was carried out to help evaluate the inhibitory effect of EOEJT against NADPH oxidase. Results of the GC/MS analysis revealed seven major compounds in EOEJT wherein muurolol (36%) and elemol (26%) were the major components. Moreover, EOEJT possessed interesting antioxidant potential with an IC50 respectively of 21.25 ± 1.02 μg/mL, 481.02 ± 5.25 μg/mL, and 271 μg EAA/mg in DPPH, FRAP, and total antioxidant capacity systems. Molecular docking of EOEJT in NADPH oxidase active site showed inhibitory activity of α-cadinol and muurolol with a glide score of −6.041 and −5.956 Kcal/mol, respectively. As regards the antibacterial and antifungal capacities, EOEJT was active against all tested bacteria and all fungi, notably, against Escherichia coli K12 with an inhibition diameter of 21 mm and a MIC value of 0.67 mg/mL, as well as against Proteus mirabilis ATCC 29906 with an inhibition diameter of 18.33 ± 1.15 mm and a MIC value of 1.34 mg/mL. A more pronounced effect was recorded for the fungal pathogens Fusarium oxysporum MTCC 9913 with inhibition of 37.44 ± 0.28% and MIC value of 6.45 mg/mL, as well as against Candida albicans ATCC 10231 with an inhibition diameter of 20.33 ± 1.15 mm and a MIC value of 0.67 ± 0.00 mg/mL. Altogether, these results highlight the importance of EOEJT as a source of natural antibacterial and antioxidant drugs to fight clinically important pathogenic strains.

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Section: Discussionmentioning

confidence: 99%

GC-MS Profiling, In Vitro Antioxidant, Antimicrobial, and In Silico NADPH Oxidase Inhibition Studies of Essential Oil of Juniperus thurifera Bark

Lafraxo

Moussaoui

Jardan

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Fungus inoculation was performed according to Ribeiro et al (2020), with some modifications. Each nut was inoculated in five different points with 5 μL of the suspension containing A. nomius (10 5 conidia mL −1 ) and kept under a laminar flow hood for 1 h to fix the fungi [ 70 ]. After this, the contaminated nuts were treated with one of the following treatments (1.0 mL): (1) optimized NLC formulations containing PEO, (2) optimized blank NLC formulations (negative control, NC), (3) aqueous solution of Tween 80 (1%, w / w ) containing 250 µg mL −1 of PEO, (4) aqueous solution of Tween 80 (1%, w / w ) containing Carbendazim at a concentration of 250 µg mL −1 (positive control, PC), or 5) a 0.9% saline solution (fungal control, FC).…”

Section: Methodsmentioning

confidence: 99%

Design of Nanostructured Lipid Carriers Containing Cymbopogon martinii (Palmarosa) Essential Oil against Aspergillus nomius

et al. 2021

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Palmarosa essential oil (PEO) is an alternative to synthetic fungicides to control the contamination by food-deteriorating fungi, such as Aspergillus nomius. Nonetheless, the low long-term stability and volatility hamper its utilization. Thus, this study aimed to develop nanostructured lipid carriers (NLCs) containing PEO to improve its stability and consequently prolong the activity against A. nomius. A mixture design was applied to find the best preparation conditions for antifungal activity. The characterization analyses included size measurements, zeta potential (ζ-potential), entrapment efficiency (EE), and antifungal activity (by inhibition of mycelial growth (IMG) and/or in situ test (pre-contaminated Brazil nuts) tests). The nanocarriers presented particle sizes smaller than 300 nm, homogeneous size distribution, ζ-potential of −25.19 to −41.81 mV, and EE between 73.6 and 100%. The formulations F5 and F10 showed the highest IMG value (98.75%). Based on the regression model, three optimized formulations (OFs) were tested for antifungal activity (IMG and in situ test), which showed 100% of inhibition and prevented the deterioration of Brazil nuts by A. nomius. The preliminary stability test showed the maintenance of antifungal activity and physicochemical characteristics for 90 days. These results suggest a promising system as a biofungicide against A. nomius.

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“…Colonies of Fusarium species were then counted by the enumeration technique. Consequently, plates containing 15 to 150 colonies were selected for counting and the result was expressed in log (CFU/g) of three replicates (Ribeiro et al, 2020). As regards the virulence of each fungal strain, it was estimated after 7 weeks based on the disease index (DI) technique using the 0-4 scale established by Hernandez et al (1998) which was based on visible symptoms on each seedling, where code 4: dead turf, code 3: 67 to 99% affected plant, code 2: 34 to 66% affected plant, code 1: 1 to 33% affected plant and code 0: no visible symptoms.…”

Section: Positive Control 30 Infested and Untreated Seedsmentioning

confidence: 99%

Potential of carvacrol as plant growth-promotor and green fungicide against fusarium wilt disease of perennial ryegrass

Saghrouchni

Barnossi²,

Mssillou³

et al. 2023

Front. Plant Sci.

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Perennial ryegrass (Lolium perenne L.) is a valuable forage and soil stabilisation crop. Perennial crops have long been associated with good environmental performance and ecosystem stability. Vascular wilt diseases caused by Fusarium species are the most damaging plant diseases affecting both woody perennials and annual crops. Therefore, the aim of the present study was the assessment of the preventive and growth-promoting effects of carvacrol against Fusarium oxysporum, F. solani, and F. nivale (phylogenetically analyzed on the basis of internal transcribed spacer (ITS) regions) causing vascular wilt of ryegrass in vitro and under greenhouse conditions. To accomplish this aim, various parameters were monitored including coleoptile development, rhizogenesis, the incidence of coleoptile lesions, disease index, the visual appearance of ryegrass health, ryegrass organic matter and soil fungal load. The results obtained showed that F. nivale was highly harmful to ryegrass seedlings compared to other Fusarium species. Furthermore, carvacrol with 0.1 and 0.2 mg/mL protected significantly the seedlings against Fusarium wilt diseases both in vitro and in the greenhouse. Simultaneously, carvacrol also functioned as a seedling growth promoter, as is reflected in all monitored parameters, such as the recovery of seedling height and root length, and the development of new leaf buds and secondary roots. Carvacrol proved to be effective plant growth promoter and a bio-fungicide against Fusarium vascular diseases.

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Efficacy of sodium hypochlorite and peracetic acid against Aspergillus nomius in Brazil nuts

Cited by 15 publications

References 25 publications

GC-MS Profiling, In Vitro Antioxidant, Antimicrobial, and In Silico NADPH Oxidase Inhibition Studies of Essential Oil of Juniperus thurifera Bark

GC-MS Profiling, In Vitro Antioxidant, Antimicrobial, and In Silico NADPH Oxidase Inhibition Studies of Essential Oil of Juniperus thurifera Bark

Design of Nanostructured Lipid Carriers Containing Cymbopogon martinii (Palmarosa) Essential Oil against Aspergillus nomius

Potential of carvacrol as plant growth-promotor and green fungicide against fusarium wilt disease of perennial ryegrass

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