Efficacy of silver nanoparticles to control flavobacteriosis caused by Flavobacterium johnsoniae in common carp Cyprinus carpio

Shaalan, Mohamed; Sellyei, Boglárka; El-Matbouli, Mansour; Székely, Csaba

doi:10.3354/dao03439

Cited by 23 publications

(15 citation statements)

References 40 publications

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“…47 The treated group The improvement in clinical signs combined with bacterial load decrease and lack of negative histopathological changes in the intestine and liver confirmed the antibacterial activity of orally administered Ag NPs against C. perfringens. These results agree with previous reports of the antibacterial effects of Ag NPs on E. coli in chickens, 49 Pseudomonas aeruginosa and Flavobacterium johnsoniae in fish, 50,51 Salmonella spp. in sheep and goats, 21 and Staphylococcus aureus and E. coli in in vitro studies.…”

supporting

confidence: 93%

Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

Salem

Ismael

Shaalan

2021

IJN

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Background: Clostridium perfringens-associated necrotic enteritis (NE) is a serious problem affecting broiler production. A major global challenge is to reduce the use of antibiotics in poultry industry due to their negative impacts on public health. One alternative is to use nanoparticles (NPs) to overcome bacterial resistance to antibiotics. Silver nanoparticles (Ag NPs) showed strong antimicrobial activity. Methods: A total of 120 Cobb broiler chicks (1-day old) were obtained for this study and were divided into 4 equal groups at age of 14 days (30 birds each); each group was subdivided into 3 equal replicates (10 birds each). The groups were designated as follows: G1, infected; G2, infected and treated with Ag NPs; G3, treated with Ag NPs; and G4, negative control. Birds were infected with 4×10 8 colony forming unit (CFU)/mL/bird C. perfringens type A for 2 successive days. In the treated groups, Ag NPs (mean diameter 15 nm; total dose 150 µg/bird) were administered via crop gavage. During the observation period (5 weeks), bird performance and immune organ indexes were recorded. Serum samples were collected for immunological evaluation, and tissue samples were collected for histopathology and estimation of Ag NPs residues. Results: Treatment with Ag NPs reduced the colonization of C. perfringens in the intestine and ceca, decreased the severity of clinical signs and reduced mortalities in comparison with infected non-treated group. Ag NPs treatment alleviated pathological lesions in the intestine and liver, but their residues were found in the muscles. Conclusion: Ag NPs have a positive impact on gut health integrity while having no impact on immune organs. Ag NPs have some residues in muscles; therefore, further studies are needed on the concentration and size of Ag NPs, the route of administration, and withdrawal time to ensure the safety of chicken meat for human consumption.

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confidence: 93%

Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

Salem

Ismael

Shaalan

2021

IJN

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“…Finally, the in vivo study declared the safety and potency of the integrated AgNPs in the treatment of MDR salmonellosis so it can serve as an alternative antimicrobial, especially at the occurrence of antibiotic-resistant bacterial infections. 74…”

Section: Dovepressmentioning

confidence: 99%

<p>The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-Resistant <em>Salmonella</em> Species Isolates</p>

Farouk

El-Molla

Salib

et al. 2020

IJN

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The main objective of this study is to investigate the antibacterial activity of silver nanoparticles (AgNPs) against multidrug-resistant Salmonella isolates recovered from diarrheic sheep and goats Methods: This study used chemical reduction synthesis of AgNPs to evaluate their antimicrobial effects by estimation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for each isolate using the microplate dilution method and tetrazolium salt reduction test to detect the viability percentage. In vivo treatment efficacy was assessed in mice by determining the viable count of Salmonella Enteritidis recovered from feces and by hematologic, biochemical and histopathologic examinations to confirm that use of AgNPs has no toxic or pathologic effects and to evaluate its ability in tissue regeneration following treatment. Results: All recovered strains were identified as MDR with a prevalence of 4% and 3.6% in sheep and goats, respectively. The results of TEM, DLS, Zeta potential, and FTIR revealed typical characteristics of the synthesized AgNPs. Silver nanoparticles showed antibacterial activity against all recovered strains with MIC of ≤0.02-0.313 μg/mL (mean average 0.085 ±0.126 μg/mL) and MBC of 0.078-1.250 μg/mL (average 0.508±0.315 μg/mL). In vivo efficacy of AgNPs was observed by a reduction in the number of viable S. Enteritidis recovered from feces in an S. Enteritidis infected mouse model, with complete shedding stopping between treatment days 4 and 6. Hematologic, serum biochemical, and histopathologic analyses proved the ability of AgNPs to suppress inflammatory reaction caused by S. Enteritidis infection. Conclusion: The study proved the effective ability of AgNPs to fight MDR Salmonella spp. in vitro and in vivo without adverse effects.

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“…Different preparations of silver nanoparticles have shown antibacterial activity against various fish pathogens such as Aeromonas salmonicida , Vibrio spp. and Flavobacterium johnsoniae (Fatima et al., 2020; Shaalan et al., 2018, 2020).…”

Section: Introductionmentioning

confidence: 99%

Green synthesis and physical properties of Gum Arabic‐silver nanoparticles and its antibacterial efficacy against fish bacterial pathogens

et al. 2020

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This study aimed to assess the antibacterial efficacy of Gum Arabic‐based silver nanoparticles against certain fish bacterial pathogens. Silver nanoparticles were green‐synthesized using Gum Arabic (AgNPs‐GA), which served as a stabilizing and reducing agent. The AgNPs‐GA were analysed using a UV spectrophotometer set at a wavelength of 450 nm. Transmission electron micrograph analysis showed that nearly all AgNPs‐GA were sphere‐shaped and 10.0 nm in diameter. Dynamic light scattering (DLS) analysis showed a narrow size distribution curve, whose highest peak was at 26.2 nm. The particles were negatively charged (−17.1 ± 4.9 mV). Silver concentration measured by inductively coupled plasma optical emission spectrometry (ICP‐OES) and found to be 104 µg/ml. The in vitro antibacterial activity of AgNPs‐GA was tested against Aeromonas hydrophila and Pseudomonas aeruginosa. Gum Arabic‐silver nanoparticles exhibited clear inhibition zones of 22 and 20 mm against A. hydrophila and P. aeruginosa, with a minimum inhibitory concentration of 1.625 µg/ml and 3.25 µg/ml for AgNPs‐GA respectively. The antibiofilm activity indicated that AgNPs‐GA significantly inhibit A. hydrophila and P. aeruginosa biofilm formation at AgNPs‐GA concentrations of 1.625 µg/ml. There was no significant difference at a AgNPs‐GA concentration of 0.8215 µg/ml between the control‐positive groups and the AgNPs‐GA treatment groups in both bacterial strains. In summary, green‐synthesized sliver nanoparticles display efficient antibacterial properties, which suggests that they would be suitable for use in a commercialized antibacterial product for the aquaculture industry.

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Efficacy of silver nanoparticles to control flavobacteriosis caused by Flavobacterium johnsoniae in common carp Cyprinus carpio

Cited by 23 publications

References 40 publications

Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

<p>The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-Resistant <em>Salmonella</em> Species Isolates</p>

Green synthesis and physical properties of Gum Arabic‐silver nanoparticles and its antibacterial efficacy against fish bacterial pathogens

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