Green synthesis and physical properties of Gum Arabic‐silver nanoparticles and its antibacterial efficacy against fish bacterial pathogens

El‐Adawy, Mahmoud M.; Eissa, Alaa Eldin; Shaalan, Mohamed; Ahmed, Ahmed A.; Younis, Nehal A.; Ismail, M. A.; Abdelsalam, Mohamed

doi:10.1111/are.14983

Cited by 31 publications

(25 citation statements)

References 32 publications

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“…47 The treated group The improvement in clinical signs combined with bacterial load decrease and lack of negative histopathological changes in the intestine and liver confirmed the antibacterial activity of orally administered Ag NPs against C. perfringens. These results agree with previous reports of the antibacterial effects of Ag NPs on E. coli in chickens, 49 Pseudomonas aeruginosa and Flavobacterium johnsoniae in fish, 50,51 Salmonella spp. in sheep and goats, 21 and Staphylococcus aureus and E. coli in in vitro studies.…”

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confidence: 93%

Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

Salem

Ismael

Shaalan

2021

IJN

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Background: Clostridium perfringens-associated necrotic enteritis (NE) is a serious problem affecting broiler production. A major global challenge is to reduce the use of antibiotics in poultry industry due to their negative impacts on public health. One alternative is to use nanoparticles (NPs) to overcome bacterial resistance to antibiotics. Silver nanoparticles (Ag NPs) showed strong antimicrobial activity. Methods: A total of 120 Cobb broiler chicks (1-day old) were obtained for this study and were divided into 4 equal groups at age of 14 days (30 birds each); each group was subdivided into 3 equal replicates (10 birds each). The groups were designated as follows: G1, infected; G2, infected and treated with Ag NPs; G3, treated with Ag NPs; and G4, negative control. Birds were infected with 4×10 8 colony forming unit (CFU)/mL/bird C. perfringens type A for 2 successive days. In the treated groups, Ag NPs (mean diameter 15 nm; total dose 150 µg/bird) were administered via crop gavage. During the observation period (5 weeks), bird performance and immune organ indexes were recorded. Serum samples were collected for immunological evaluation, and tissue samples were collected for histopathology and estimation of Ag NPs residues. Results: Treatment with Ag NPs reduced the colonization of C. perfringens in the intestine and ceca, decreased the severity of clinical signs and reduced mortalities in comparison with infected non-treated group. Ag NPs treatment alleviated pathological lesions in the intestine and liver, but their residues were found in the muscles. Conclusion: Ag NPs have a positive impact on gut health integrity while having no impact on immune organs. Ag NPs have some residues in muscles; therefore, further studies are needed on the concentration and size of Ag NPs, the route of administration, and withdrawal time to ensure the safety of chicken meat for human consumption.

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confidence: 93%

Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

Salem

Ismael

Shaalan

2021

IJN

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“…The deterioration of the aquatic ecosystem could be implicated as major stressor that triggered massive microbial invasion (Abu‐Elala et al, 2015; Eissa, 2016; El‐Jakee et al, 2020; Mahmoud et al, 2016). Various infectious diseases including photobacteriosis, vibriosis and streptococcosis were incriminated in numerous outbreaks in wild and farmed fish (Abdelsalam et al, 2009; Abdelsalam et al, 2015; Abdelsalam et al, 2017; Eissa et al, 2020; Eissa et al, 2016; Eissa, Abolghait, et al, 2020; El‐Adawy et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Catastrophic mass mortalities caused by Photobacterium damselae affecting farmed marine fish from Deeba Triangle, Egypt

Eissa

Abou‐Okada

Alkurdi

et al. 2021

Aquaculture Research

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Farmed marine fish constitute 20% of the total farmed fish production in Egypt, and the Deeba Triangle produces a relatively large portion of this percentage. Last year, several private fish farms in the Deeba Triangle have suffered severe economic losses due to acute fish mass kills. This study aimed to investigate the hidden aetiologies behind these colossal mass fish kills and to propose an emergency control strategy. Several tons of dead farmed fish were remarkably scattered throughout affected ponds and at the vicinity of impacted fish farms. Moribund farmed European seabass (Dicentrarchus labrax), thin‐lipped grey mullet (Liza ramada) and gilthead seabream (Sparus aurata) have exhibited skin darkness, emaciation, congested gills and fins, ascites, skin erosions and ulcerations. Internally, moribund fish emitted unpleasant odour upon opening the abdomen together with severe congestion and haemorrhages in kidneys and brain. Mottled atrophied spleens were the most prominent findings, while the gastrointestinal tracts were filled with whitish caseous material. The liver was pale with multiple whitish nodules. Photobacterium damselae was the most retrievable bacterial pathogen from most infected fish and trash fish. Photobacterium damselae subspecies piscicida and Photobacterium damselae subspecies damselae were definitively identified from examined moribund fish using both conventional morpho‐chemical and molecular assays. Data analysis has revealed that the poor water quality was profoundly incriminated in triggering the bacterial infections with a fate of mass mortalities. Conclusively, adopting various strict biosecurity strategies will be the key factors in prevention of future episodes of mass kills.

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“…AgNPs were chemically synthesized and primarily confirmed by changing the colour of the reaction mixture from colourless to brown colour (Rajkumar et al, 2016). In the present study, the synthesized AgNPs are nano-sized, spherical, negatively charged particles that increase the antibacterial activity and stability of AgNPs (Liu et al, 2016 andEl-Adawy et al, 2020). Using high polyvinylpyrrolidone (PVP) concentrations for coating AgNPs resulted in smaller AgNPs (Jiang et al, 2006).…”

Section: Discussionmentioning

confidence: 79%

“…The nanoparticles such as silver nanoparticles and zinc oxide nanoparticles were believed to be a hope for possible application as an antimicrobial agent instead of the traditional antibiotics (Shimanovich & Gedanken, 2016). Applications of silver nanoparticles to improve the water quality of fish ponds and treatment of wastewaters have gained wide attention (El-Adawy et al, 2020;Moustafa, 2017).…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles as an antibacterial agent in Oreochromis niloticus and Sparus auratus fish

et al. 2021

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A trial was carried out to assess the potential use of chemically synthesized silver nanoparticles (AgNPs) with two concentrations (5 and 10 µg/L) as an antibacterial agent in Oreochromis niloticus and Sparus auratus against Streptococcus agalactiae and Vibrio alginolyticus respectively. All AgNPs were with a mean of 26 nm in diameter.The particles were negatively charged (−30.7 ± 0.45 mV). In different ways, the in vitro AgNP antibacterial activity was investigated against S. agalactiae and V. alginolyticus in different ways showing clear inhibition zones of the two AgNP concentrations dose-dependent manner. In vitro measurement of extracellular proteins of pathogenic S. agalactiae and V. algenolyticus against AgNPs and the ultrastructural interaction between AgNPs and these bacterial pathogens using transmission electron microscopy (TEM) revealed AgNPs have good antibacterial properties. O. niloticus and S. auratus were exposed to AgNPs for 28 days. Some groups were challenged with S. agalactiae and V. alginolyticus respectively. Haematological, biochemical and immunological parameters and phagocytic activity, phagocytic index, respiratory burst activity of neutrophils and serum lysozyme activity were estimated in addition to the concentration of minerals. Histopathological examinations of all fish groups were explored. From the investigation above, we concluded that AgNPs have significant deleterious effects on O. niloticus exposed to 5 and 10 µg/L of AgNPs, appearing a significant decrease in leukocytes, red blood corpuscles (RBCs) count and haemoglobin concentration the fish on day 28 of the trial, with more deleterious effect with challenging fish by S. agalactiae and V. algenolyticus. In addition, AgNPs caused a substantial increase in liver enzymes with a reduction in total protein, albumin and globulin in the fish and considerably decreased the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Additionally, there was a significant decrease in sodium (Na) and potassium (K) content, nitroblue tetrazolium (NBT) and lysozyme activity (LYZ) in all groups of O. niloticus and S. auratus. In addition, lower survival rates disturb histopathological findings; meanwhile, S. auratus exposed to 5 and 10 µg/L of AgNPs showed antibacterial potency and immunostimulatory effects, which appeared in their higher survival rates and induced immune parameters and histopathological findings. In low concentration (5 µg/ml of AgNPs), AgNPs were less cytotoxic, especially in S. auratus, | 6219 MOUSTAFA eT Al.

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Green synthesis and physical properties of Gum Arabic‐silver nanoparticles and its antibacterial efficacy against fish bacterial pathogens

Cited by 31 publications

References 32 publications

Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

Evaluation of the Effects of Silver Nanoparticles Against Experimentally Induced Necrotic Enteritis in Broiler Chickens

Catastrophic mass mortalities caused by Photobacterium damselae affecting farmed marine fish from Deeba Triangle, Egypt

Silver nanoparticles as an antibacterial agent in Oreochromis niloticus and Sparus auratus fish

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