Efficacy of reciprocating and ultrasonic activation of 6% sodium hypochlorite in the reduction of microbial content and virulence factors in teeth with primary endodontic infection

Abstract

Aveiro E, Chiarelli-Neto VM, de-Jesus-Soares A, Zaia AA, Ferraz CCR, Almeida JFA, Marciano MA, Feres M, Gomes BPFA. Efficacy of reciprocating and ultrasonic activation of 6% sodium hypochlorite in the reduction of microbial content and virulence factors in teeth with primary endodontic infection.

Investigation of microbial profile, levels of endotoxin and lipoteichoic acid in teeth with symptomatic irreversible pulpitis: a clinical study

“…Previous studies have described the methods for clinical and sampling procedures, microbiological assessment using checkerboard DNA-DNA hybridization and quantification of the levels of LPS and LTA (Socransky et al 1994, Vianna et al 2008, Duque et al 2019, Aveiro et al 2020, Louzada et al 2020.…”

“…Clinical procedures and sample collection were performed as is described in previous studies (Barbosa-Ribeiro et al 2019, Aveiro et al 2020, Louzada et al 2020. Briefly, patient's facial skin was decontaminated with 2% CHX solution (Drogal, Piracicaba, SP, Brazil) prior to intervention.…”

“…Microbiological assessment using checkerboard DNA-DNA hybridization (CB) was performed in accordance with previous studies (Socransky et al 1994, Vianna et al 2008, Aveiro et al 2020, Louzada et al 2020.…”

“…Previous studies have evaluated the microbiota of dentinal caries (Aas et al 2008, Rôc ßas et al 2015, Zheng et al 2019. Others have investigated the infectious content from root canals in various clinical situations (Martinho et al 2014, Barbosa-Ribeiro et al 2016, Duque et al 2019, Aveiro et al 2020, Louzada et al 2020, Machado et al 2020. However, few studies have evaluated the effects of root canal procedures on the infectious content in teeth with irreversible pulpitis.…”

Influence of voxel size on micro-CT analysis of debris after root canal preparation

“…19 Ultrasonic activation with Irrisonic promoted greater reduction in microbial load within root canals. 20 To the best of our knowledge, no studies have been performed to date that evaluate the influence of voxel size on the analysis of debris accumulation after root canal preparation. Therefore, the aim of this study was to assess the influence of voxel size in micro-CT evaluation of debris accumulation after PUI application in curved root canals prepared with PDL or HEDM.…”