Efficacy of oxytetracycline and potentiated sulphonamide oral therapies against Aeromonas hydrophila infection in Nile tilapia Oreochromis niloticus

Abraham, Thangapalam Jawahar; Roy, Anwesha; Julinta, Roy Beryl; Singha, Jasmine; Patil, Prasanna Kumar

doi:10.12980/jclm.5.2017j7-89

Cited by 8 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, multiple NB‐O 3 treatments were effective with RPS of 64.7%–66.7%. The RPS value in this study was similar or higher than several studies using antibiotics for Aeromonads control in Nile tilapia, for example RPS of 60% in orally administered fish with oxytetracycline 4 g/kg/feed per day (Abraham et al., 2017) or RPS 25.9% in orally fed fish with oxytetracycline 60 mg/kg biomass per day (Julinta et al., 2017).…”

Section: Discussionsupporting

confidence: 84%

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila

Dien

Linh

Sangpo

et al. 2021

Journal of Fish Diseases

View full text Add to dashboard Cite

A rapid increase in multi‐drug‐resistant (MDR) bacteria in aquaculture highlights the risk of production losses due to diseases and potential public health concerns. Previously, we reported that ozone nanobubbles (NB‐O3) were effective at reducing concentrations of pathogenic bacteria in water and modulating fish immunity against pathogens; however, multiple treatments with direct NB‐O3 exposures caused alterations to the gills of exposed fish. Here, we set up a modified recirculation system (MRS) assembled with an NB‐O3 device (MRS‐NB‐O3) to investigate whether MRS‐NB‐O3 (a) were safe for tilapia (Oreochromis niloticus), (b) were effective at reducing bacterial load in rearing water and (c) improved survivability of Nile tilapia following an immersion challenge with a lethal dose of MDR Aeromonas hydrophila. The results showed no behavioural abnormalities or mortality of Nile tilapia during the 14‐day study using the MRS‐NB‐O3 system. In the immersion challenge, although high bacterial concentration (~2 × 107 CFU/ml) was used, multiple NB‐O3 treatments in the first two days reduced the bacteria between 15.9% and 35.6% of bacterial load in water, while bacterial concentration increased from 13.1% to 27.9% in the untreated control. There was slight up‐regulation of non‐specific immune‐related genes in the gills of the fish receiving NB‐O3 treatments. Most importantly, this treatment significantly improved survivability of Nile tilapia with relative percentage survival (RPS) of 64.7% – 66.7% in treated fish and surviving fish developed specific antibody against MDR A. hydrophila. In summary, the result suggests that NB‐O3 is a promising non‐antibiotic approach to control bacterial diseases, including MDR bacteria, and has high potential for application in recirculation aquaculture system (RAS).

show abstract

Section: Discussionsupporting

confidence: 84%

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila

Dien

Linh

Sangpo

et al. 2021

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…The RPS value in this study was similar or higher than several studies using antibiotics for Aeromonads control in Nile tilapia e.g. RPS of 60% in orally administered with Oxytetracycline 4g/kg/feed per day (Abraham et al, 2017) or RPS 25.9 % in orally fed with Oxytetracycline 60 mg/kg biomass per day (Julinta et al, 2017).…”

Section: Disscussionsupporting

confidence: 81%

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multidrug-resistantAeromonas hydrophila

Dien

Linh

Sangpo

et al. 2021

Preprint

View full text Add to dashboard Cite

Multidrug-resistant (MDR) bacteria has rapidly increased in aquaculture, which highlights the risk of production losses due to diseases and potential public health concerns. Previously, we reported that ozone nanobubbles (NB-O3) were effective at reducing concentrations of pathogenic bacteria in water and modulating fish immunity against pathogens; however, multiple treatments with direct NB-O3 exposures caused alterations to the gills of exposed-fish. Here, we set up a modified recirculation system (MRS) assembled with an NB-O3 device (MRS-NB-O3) to investigate whether MRS-NB-O3 were 1) safe for tilapia (Oreochromis niloticus), 2) effective at reducing bacterial load in rearing water, and 3) improved survivability of Nile tilapia following an immersion challenge with a lethal dose of MDR Aeromonas hydrophila. The results indicated no behavioral abnormalities or mortality of Nile tilapia during the 14-day study using the MRS-NB-O3 system. In the immersion challenge, although high bacterial concentration (~2 × 107 CFU/mL) was used, multiple NB-O3 treatments in the first two days reduced the bacteria between 15.9% to 35.6% of bacterial load in water while bacterial concentration increased 13.1% to 27.9% in the untreated control. There was slight up-regulation of non-specific immune-related genes in the gills of the fish receiving NB-O3 treatments. Most importantly, this treatment significantly improved survivability of Nile tilapia with relative percent survival (RPS) of 64.7 - 66.7% in treated fish and surviving fish developed specific antibody against MDR A. hydrophila. In summary, the result suggests that NB-O3 is a promising alternative to antibiotics to control bacterial diseases, including MDR bacteria, and has high potential for application in recirculation aquaculture system (RAS).

show abstract

“…However, it is likely that the resistance seen against tetracycline, sulfonamide and trimethoprim could have been acquired from treatment of diseased fish using these antibiotics as reported from clinical reports. Moreover, these antibiotics are widely used in aquaculture in India and resistance based on disc diffusion test has been reported previously (Abraham et al, 2017;Roy et al, 2021;Sivaraman et al, 2021;Patil et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles

et al. 2022

View full text Add to dashboard Cite

Aeromonas species are Gram-negative bacteria that infect various living organisms and are ubiquitously found in different aquatic environments. In this study, we used whole genome sequencing (WGS) to identify and compare the antimicrobial resistance (AMR) genes, integrons, transposases and plasmids found in Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii isolated from Indian major carp (Catla catla), Indian carp (Labeo rohita), catfish (Clarias batrachus) and Nile tilapia (Oreochromis niloticus) sampled in India. To gain a wider comparison, we included 11 whole genome sequences of Aeromonas spp. from different host species in India deposited in the National Center for Biotechnology Information (NCBI). Our findings show that all 15 Aeromonas sequences examined had multiple AMR genes of which the Ambler classes B, C and D β-lactamase genes were the most dominant. The high similarity of AMR genes in the Aeromonas sequences obtained from different host species point to interspecies transmission of AMR genes. Our findings also show that all Aeromonas sequences examined encoded several multidrug efflux-pump proteins. As for genes linked to mobile genetic elements (MBE), only the class I integrase was detected from two fish isolates, while all transposases detected belonged to the insertion sequence (IS) family. Only seven of the 15 Aeromonas sequences examined had plasmids and none of the plasmids encoded AMR genes. In summary, our findings show that Aeromonas spp. isolated from different host species in India carry multiple AMR genes. Thus, we advocate that the control of AMR caused by Aeromonas spp. in India should be based on a One Health approach.

show abstract

Efficacy of oxytetracycline and potentiated sulphonamide oral therapies against Aeromonas hydrophila infection in Nile tilapia Oreochromis niloticus

Cited by 8 publications

References 8 publications

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multidrug-resistantAeromonas hydrophila

Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles

Contact Info

Product

Resources

About