Efficacy of Natural Herbal Formulation against Acute Hepatopancreatic Necrosis Disease (AHPND) causing Vibrio parahaemolyticus in Penaeus vannamei

Abstract

A formulation was developed using combination of blended natural essential oils as an antiVibrio parahemolyticus causing acute hepatopancreatic necrosis disease (AHPND) candidate. Lavandula latifolia, Pinus sylvestris, Jasminum officinale, Citrus limon, Prunus avium, Viola odorata, Gardenia jasminoides, Cocos nucifera, Rosa damascene and Eucalyptus globulus, mixed together to develop as anti-V. parahemolyticus product. The treatment group was fed on essential oil mixed feed whereas control group were fed on the regular feed throughout the experiment. The shrimp of both treatment and control were challenged by immersion method at day 8. The cumulative AHPND-gross sign appearance in positive control reached up to 95% at dpi 10 whereas no gross sign appeared in treatment and in negative control. The cumulative mortality reached up to 46.7% at dpi 10 in positive controls whereas no mortality recorded in treatment and in negative control. The V. parahaemolyticus isolated from the hepatopancreas of infected shrimp matched 100% with the existing AHPND strain. The trial results show that the developed natural herbal formulation has significant effect against AHPND in a controlled condition.

Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture

“…The rose myrtle, Rhodomyrtus tomentosa , seed extract shown significantly high antimicrobial activity against AHPND bacteria. In addition, the extract was found to improve the survival of L. vannamei against AHPND-causing V. parahaemolyticus strain [ 121 ]. Later, a study was carried out to determine the effect of plant extract, Phyllanthus amarus , against AHPND-causing V. parahaemolyticus strain in white leg shrimp, L. vannamei .…”

“…In another study, essential oil mixture prepared from 10 plants, i.e., Lavandula latifolia , Pinus sylvestris , Jasminum officinale , Citrus limon , Prunus avium , Viola odorata , Gardenia jasminoides , Cocos nucifera , Rosa damascene and Eucalyptus globulus , were tested for anti- V. parahaemolyticus activity. The essential oil mixture was found to exhibit antimicrobial activity and significantly improve the survival of L. vanaamei against AHPND-causing V. parahaemolyticus strain [ 121 ]. Moreover, seaweeds are also reported to display antimicrobial activity against bacterial pathogen and possess several health-benefiting properties.…”

Effect of Solanum procumbens Lour. Extract on Survival, Growth Performances, Immune Responses and against Vibrio parahaemolyticus causing Acute Hepatopancreas Necrosis Disease in White Leg Shrimp (Litopenaeus vannamei)

“…Other research also noted that plant extracts can protect shrimp from AHPND infection. Jha et al (2016)…”

“…Antibiotics have been applied widely in aquaculture to control diseases in aquatic animals, yet the use of antibiotics might cause negative effects such as antibiotic resistance of bacteria and residual of antibiotics in aquaculture products (Lulij wa et al, 2019). Several studies demonstrated medicinal plants could be used as an alternative solution to antibiotics because they contain activate molecules such as alkaloids, terpenoids, saponins and flavonoids which can provide antibacterial, antifungal, antiviral and antiparasitic effects, growth enhancement and immunostimulation (Pu et al, 2017, Jha et al, 2016, Babikian et al, 2019.…”

Synergistic inhibition effects of tea polyphenols as adjuvant of oxytetracycline on Vibrio parahaemolyticus and enhancement of Vibriosis resistance of Exopalaemon carinicauda

“…The combination of red ginseng saponins with kanamycin or cefotaxime had synergistic or additive effects on S. aureus resistance to methicillin (Sung & Lee, 2008). In addition, blended natural essential oil, seaweed, onion and garlic also had synergistic effects when combined with antibiotics (Jha et al, 2017;Olusola et al, 2013;Vatsos & Rebours, 2015). However, the studies on the combination of NPEs and antibiotics are still being explored.…”