Efficacy of modified Leeming-Notman media in a resazurin microtiter assay in the evaluation of in-vitro activity of fluconazole against Malassezia furfur ATCC 14521

Far, F.E.; Al-Obaidi, Mohanad; Desa, Mohd Nasir Mohd

doi:10.1016/j.mycmed.2018.04.007

Cited by 17 publications

(10 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each yeast cell suspension was inoculated into 96-well microplates that had previously been prepared with the dilutions of the Copaifera samples, which was followed by incubation at 37 °C for 48 h. The wells containing only inoculum and culture medium were considered as 100% organism viability. The addition of 0.015% aqueous rezasurin solution and further incubation at 37 °C for 3 h helped to visualize yeast viability [ 16 ]. The minimum inhibitory concentration (MIC) was defined as the lowest concentration of antifungal agent that maintained blue staining in the supernatant medium.…”

Section: Methodsmentioning

confidence: 99%

Brazilian Copaifera Species: Antifungal Activity against Clinically Relevant Candida Species, Cellular Target, and In Vivo Toxicity

Andrade

Orlando

Scorzoni

et al. 2020

JoF

View full text Add to dashboard Cite

Plants belonging to the genus Copaifera are widely used in Brazil due to their antimicrobial properties, among others. The re-emergence of classic fungal diseases as a consequence of antifungal resistance to available drugs has stimulated the search for plant-based compounds with antifungal activity, especially against Candida. The Candida-infected Caenorhabditis elegans model was used to evaluate the in vitro antifungal potential of Copaifera leaf extracts and trunk oleoresins against Candida species. The Copaifera leaf extracts exhibited good antifungal activity against all Candida species, with MIC values ranging from 5.86 to 93.75 µg/mL. Both the Copaifera paupera and Copaifera reticulata leaf extracts at 46.87 µg/mL inhibited Candida glabrata biofilm formation and showed no toxicity to C. elegans. The survival of C. glabrata-infected nematodes increased at all the tested extract concentrations. Exposure to Copaifera leaf extracts markedly increased C. glabrata cell vacuolization and cell membrane damage. Therefore, Copaifera leaf extracts are potential candidates for the development of new and safe antifungal agents.

show abstract

Section: Methodsmentioning

confidence: 99%

Brazilian Copaifera Species: Antifungal Activity against Clinically Relevant Candida Species, Cellular Target, and In Vivo Toxicity

Andrade

Orlando

Scorzoni

et al. 2020

JoF

View full text Add to dashboard Cite

show abstract

“…The C. parapsilosis ATCC 22019 and C. krusei ATCC 6258 strains and AMB were included as quality control [39]. The minimum inhibitory concentration (MIC) was determined with the fluorometric indicator resazurin at 0.01% (w/v) [40]. MIC was defined as the lowest antifungal/product concentration that maintained a blue hue.…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

Rds

Andrade

et al. 2019

Plants

View full text Add to dashboard Cite

Candidiasis therapy, especially for candidiasis caused by Candida non-albicans species, is limited by the relatively reduced number of antifungal drugs and the emergence of antifungal tolerance. This study evaluates the anticandidal activity of 41 plant-derived products against Candida species, in both planktonic and biofilm cells. This study also evaluates the toxicity and the therapeutic action of the most active compounds by using the Caenorhabditis elegans–Candida model. The planktonic cells were cultured with various concentrations of the tested agents. The Cupressus sempervirens, Citrus limon, and Litsea cubeba essential oils as well as gallic acid were the most active anticandidal compounds. Candida cell re-growth after treatment with these agents for 48 h demonstrated that the L. cubeba essential oil and gallic acid displayed fungistatic activity, whereas the C. limon and C. sempervirens essential oils exhibited fungicidal activity. The C. sempervirens essential oil was not toxic and increased the survival of C. elegans worms infected with C. glabrata or C. orthopsilosis. All the plant-derived products assayed at 250 µg/mL affected C. krusei biofilms. The tested plant-derived products proved to be potential therapeutic agents against Candida, especially Candida non-albicans species, and should be considered when developing new anticandidal agents.

show abstract

“…The MIC of the fengycins was detected for A. solani using a microdilution method, with some modifications ( Wei et al, 2017 ; Far et al, 2018 ). The PDB (100 μl/well) was dispensed in each well of a 96-well plate and mixed with fengycins (100 μl/well) and continuously diluted two times with methanol, resulting in final concentrations of fengycins from 0.49 to 250 μg/mL.…”

Section: Methodsmentioning

confidence: 99%

“…After incubation for 12 h at 25°C, 0.07 g/L resazurin was added to all the wells and incubated for another 4 h to generate clear-cut endpoints. Resazurin was a non-toxic redox dye that has been used extensively to test bacteria and fungi in chemical sensitivity assays ( Wei et al, 2017 ; Far et al, 2018 ). By visual inspection, a change in color from blue (oxidized state) to pink (reduced state) indicated the growth of fungus, and the MIC was defined as the minimum inhibitory concentration of the compound that prevented this color change.…”

Section: Methodsmentioning

confidence: 99%

Biocontrol and Action Mechanism of Bacillus subtilis Lipopeptides’ Fengycins Against Alternaria solani in Potato as Assessed by a Transcriptome Analysis

et al. 2022

View full text Add to dashboard Cite

Alternaria solani is an airborne fungus and the primary causal agent of potato early blight worldwide. No available fungicides that are both effective and environmentally friendly are usable to control this fungus. Therefore, biological control is a potential approach for its suppression. In this study, Bacillus subtilis strain ZD01’s fermentation broth strongly reduced A. solani pathogenicity under greenhouse conditions. The effects of strain ZD01’s secondary metabolites on A. solani were investigated. The exposure of A. solani hyphae to the supernatant resulted in swelling and swollen sacs, and the ZD01 supernatant reduced A. solani conidial germination significantly. Matrix-assisted laser desorption/ionization time of flight mass spectrometry and pure product tests revealed that fengycins were the main antifungal lipopeptide substances. To elucidate the molecular mechanism of the fengycins’ biological control, RNA sequencing analyses were performed. A transcriptome analysis revealed that 304 and 522 genes in A. solani were differentially expressed after 2-h and 6-h fengycin treatments, respectively. These genes were respectively mapped to 53 and 57 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. In addition, the most enriched KEGG pathway analysis indicated that the inhibitory mechanisms of fengycins against A. solani regulated the expression of genes related to cell wall, cell membrane, transport, energy process, protein synthesis and genetic information. In particular, cell wall and cell membrane metabolism were the main processes affected by fengycin stress. Scanning and transmission electron microscope results revealed hyphal enlargement and a wide range of abnormalities in A. solani cells after exposure to fengycins. Furthermore, fengycins induced chitin synthesis in treated cells, and also caused the capture of cellular fluorescent green labeling and the release of adenosine triphosphate (ATP) from outer membranes of A. solani cells, which may enhance the fengycins ability to alter cell membrane permeability. Thus, this study increases the transcriptome data resources available and supplies a molecular framework for B. subtilis ZD01 inhibition of A. solani HWC-168 through various mechanisms, especially damaging A. solani cell walls and membranes. The transcriptomic insights may lead to an effective control strategy for potato early blight.

show abstract

Efficacy of modified Leeming-Notman media in a resazurin microtiter assay in the evaluation of in-vitro activity of fluconazole against Malassezia furfur ATCC 14521

Cited by 17 publications

References 29 publications

Brazilian Copaifera Species: Antifungal Activity against Clinically Relevant Candida Species, Cellular Target, and In Vivo Toxicity

Brazilian Copaifera Species: Antifungal Activity against Clinically Relevant Candida Species, Cellular Target, and In Vivo Toxicity

In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

Biocontrol and Action Mechanism of Bacillus subtilis Lipopeptides’ Fengycins Against Alternaria solani in Potato as Assessed by a Transcriptome Analysis

Contact Info

Product

Resources

About