Efficacy of Lyophilised Platelet-Rich Plasma Powder on Healing Rate in Patients With Deep Second Degree Burn Injury

Abstract

Platelet-rich plasma (PRP) is a kind of plasma that is rich in platelets after processing. It includes various growth factors and cytokines, which speed up the process of wound healing and hemostasis. The PRP solution used in this study is diluted from lyophilized PRP powder, which decreased the possibility of contamination, facilitated the storage, and prolonged the storage life. From in vitro fibroblast proliferation testing, the numbers of PRP supplement were performed for 1, 4, and 7 times by continuous replacement of culture medium each day. Four times of lyophilized PRP supplement was selected for clinical study due to sufficient promotion of fibroblast proliferation. Next, 27 patients of deep second-degree burn wound were included in this study. Patients were assigned to two groups: PRP group (n = 15) and control group (n = 12). A concentration of 1.0 × 10 platelets/cm (wound area) according to wound size was sprayed on the wound evenly. Function was mainly assessed by the percentage of wound closure and bacteria picking out rate in 2 and 3 weeks. The wound closure at 3 weeks showed a significant difference in PRP group (P < 0.05). The healing rate of PRP group reached nearly 80% and made a breakthrough of 90% in 3 weeks, showing a significant difference compared with the control group (P < 0.05). Lyophilized PRP can be considered as an effective treatment to increase healing rate in patients with deep second-degree burn injury.

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

“…The use of PRP in specific wound etiologies, such as diabetic foot, is based on high quality clinical evidence [36], but clinical data reporting the efficacy of FD-PRP are limited. FD-PRP powder (1 × 10 7 platelets/cm 2 ) was used in the management of deep second degree burn wounds in the plantar area showing significant beneficial differences in the healing rate at 3 weeks and bacteria colonization compared to controls [37].…”

Fractional carbon dioxide laser‐assisted delivery of lyophilized‐growth factors is a promising treatment modality of post‐acne scars

“…Recently, L-GFs have been used in different fields including ophthalmology treating ocular surface disorders, 9 orthopedic surgery for bone tissue regeneration, 10 and in plastic surgery treating chronic wounds 11 as well as acute wounds of burns. 12 It has not been used widely in dermatological fields. To the best of our knowledge, no T A B L E 5 Comparison between both treatment sides of the face according to the degree of PIH and post-treatment downtime…”

Utilization of lyophilized platelet-rich plasma in esthetic facial treatment

“…For routine PRP preparation, patients have to have blood drawn each time before centrifugation due to the short shelf life of PRP [10]. For L-PRP preparation, 250 ml of autologous blood was collected at once.…”