Efficacy of indigenous probiotic Lactobacillus strains to reduce cadmium bioaccessibility - An in vitro digestion model

Abstract

The toxic heavy metal cadmium (Cd) appears as one of the major global threats to human and animal health. Human being and aquatic life are exposed to Cd by breathing, eating, or drinking when industrial effluents released into environment. The study was aimed to identify cadmium-binding Lactobacillus strain to reduce its bioaccessibility in in vitro digestion model. In this context, forty-eight lactobacilli strains isolated and characterized from fermented dairy products and human origin were screened for their Cd biosorption potential using Flame Atomic Absorption Spectroscopy (FAAS). The present study revealed that Cd biosorption potential of 48 lactobacilli strains ranged from 1.0832 ± 0.012 to 3.562 ± 0.03 mg Cd g of cells from initial 10 mg L cadmium chloride (CdCl) aqueous solution. Lactobacillus plantarum strain HD 48 demonstrated highest biosorption of 3.562 ± 0.03 mg Cd g of cells. Lactobacilli-Cd complex stability indicated its strong stability as even after three washes with Milli-Q water metal desorption was nonsignificant (p < 0.05) and further studies to delineate the influence of Cd (100 mg L CdCl) on their growth. Moreover, these strains were able to reduce Cd bioaccessibility in the in vitro digestion model in the range of 24.71 to 41.62 %. Transmission electron microscopy (TEM) investigations on Cd bioadsorption also revealed its surface associated bioadsorption phenomenon. These findings depicted that probiotic strain L. plantarum HD 48 was found to be endowed with remarkable Cd biosorption ability as well as reduction in its bioaccessibility. These results suggest that probiotic strain L. plantarum HD 48 has immense potential to sequester Cd from aqueous solution which could be further explored as a potent source to diminish body Cd burden.

In vitro and in vivo evaluation of Weissella cibaria and Lactobacillus plantarum for their protective effect against cadmium and lead toxicities

“…; Kumar et al . ). The results from this study show that binding conditions of our strains to cadmium and lead toxicities is due to the freshly cultured nature of the strains.…”

“…; Kumar et al . ). Cd and Pb exposure affects delicate part of human body, which causes various toxic disorders including skeletal and cardiovascular dysfunctions (Bhakta et al .…”

“…; Kumar et al . ). Humans are subject to exposure of cadmium and lead pollution through contamination of air that we breathe, food from tainted soil and water from pipelines, manufactured goods and occupational hazards.…”

“…, ; Kumar et al . ). The aim of this study was to screen probiotic lactic acid bacteria from two Nigerian traditional foods (fermented cassava mash to make gari and wara (African soft cheese) for their in vitro and in vivo protective capacities against acute cadmium and lead toxicities.…”

Health promoting activities of probiotics

“…However, sterilization might do damage to physical structure of cell wall, which obviously decreases binding abilities of dead probiotics (Schut, Zauner, Hampel, König, & Claus, ). Several other reports revealed that some probiotic strains belonging to L. plantarum could bind to heavy metals possibly by forming Lactobacilli ‐Cd complex or altering cell components of membranes (Kumar, Kumar, Panwar, & Ram, ; Tong et al, ).…”