Chlorococcum is a coccoid green algal genus, which contains almost 50 species. The genus is polyphyletic, but in a traditional sense, the cells are coccoid and non-motile with a cup-shape chloroplast in the vegetative stage, while the spores have chlamydomonad appearance. They are distributed worldwide mainly in terrestrial habitats, and the biotechnological potential of several strains has also been reported. In this study, three new green algal strains from a shallow temporary freshwater lake in Hungary are characterized using microscopic (light and transmission electron microscopy) and DNA-based methods (phylogenetic analysis of the ribosomal ITS region, the 18S ribosomal RNA and rbcL genes, and ITS secondary structure comparison). Based on the obtained results, one of the three new isolates is considered to represent a new species, which is described here as Chlorococcum szentendrense sp. nov.