Efficacy of different instrumentation techniques on reducing Enterococcus faecalis infection in experimentally infected root canals

Demiryürek, Ebru Özsezer; Kalyoncuoğlu, Elif; Duran, Esra; Çoban, Ahmet Yılmaz; Çaycı, Yeliz Tanrıverdi

doi:10.1016/j.jds.2012.03.024

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…Chemomechanical preparation of the root canal is considered the first step to reach the target of eradication of the intracanal bacterial invasion [17]. The chemomechanical procedures can reduce endodontic infection rather than ensure an immaculate root canal system; hence, microorganisms can survive inside the complex anatomy of the root canal system [18,19]. Therefore, the intracanal medicaments represent an ideal reinforcement step to achieve the complete disinfection of the root canal system [20].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics

Abdulrahman

Faraj

Dizaye

2020

BioMed Research International

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Background and Objectives. Multiple antibacterial agents have been mixed and used as an intracanal medicament-like modified triple antibiotic paste (MTAP) to eliminate Enterococcus faecalis (EF), which has been most frequently identified in the cases of failed root canal treatment and periapical lesions. This study is aimed at using a single antibacterial agent, nitrofurantoin (Nit), as an experimental intracanal medicament paste against different clinical isolates of EF bacteria and at comparing its antimicrobial efficacy with MTAP. Materials and Methods. Three strains of EF (S1, S2, and S3) were clinically isolated. A total of 198 straight single-rooted human teeth were collected and divided randomly into three main groups: group N (Nit) (n=90), group M (MTAP) (n=90), and group W (distilled water) (n=18). The main groups were subdivided into three subgroups according to the strain of EF: in groups N and M, subgroups S1, S2, and S3 (n=30), while in group W, subgroups S1, S2, and S3 (n=6). Then, each subgroup of N and M was divided into five groups (n=6) according to the concentrations of Nit or MTAP (6.25, 12.5, 25, 50, and 100 mg/mL). The colony-forming unit (CFU) of EF from the canal lumen and dentinal chips was measured. Results. Nit could eradicate S1, S2, and S3 completely with concentrations of 6.25, 12.5, and 25 mg/mL, respectively, while MTAP showed complete eradication of the three strains only at 25 mg/mL. In all the groups, it was found that the CFU counts of EF in the dentinal chips were higher than those in the root canal lumen. Conclusion. At the concentration of 25 mg/mL, the Nit paste is effective in eradicating EF completely when it is used as an intracanal medicament.

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Section: Introductionmentioning

confidence: 99%

Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics

Abdulrahman

Faraj

Dizaye

2020

BioMed Research International

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“…4,5 Recent studies have suggested that the presence of Enterococcus faecalis in the root canal can increase the risk of endodontic infections during or after the root canal treatment. 6,7,8 Studies on NaOCl and chlorhexidine as antimicrobial irrigants have shown that both irrigants are effective to be used as antibacterial agents. 9,10 However, NaOCl is potentially toxic to human osteoblast cells; such toxicity can affect tissue regeneration, thereby causing a delay in the healing process.…”

Section: Introductionmentioning

confidence: 99%

Comparing the effectivities of chitosan citrate and chitosan acetate in eradicating Enterococcus faecalis biofilm

et al. 2018

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Background: Adequate biomechanical preparations, antibacterial irrigants, and intracanal medications to promote the elimination of bacteria and their products are required to succeed root canal treatment. Enterococcus faecalis with its biofilm is known as an important etiological agent in endodontic treatment failures. Chitosan, as a natural product, has an antibacterial activity and is considered less toxic to the periapical tissue than other irrigants. However, the use of this natural product needs to be examined to determine its effectiveness as a root canal irrigant in endodontic treatment; this can be done by comparing it with the most common endodontic irrigant (NaOCl 5.25%) as a positive control. Objective: The objective of the study was to compare the effectiveness between 1-3% chitosan acetate (CA) and 1-3% chitosan citrate (CC) against E. faecalis biofilm formation after treatment for 15, 30, and 60 minutes. Methods: The study was conducted using 12 groups, including 1-3% CA, 1-3% CC, and control groups. E. faecalis biofilms in 96-well plates were exposed to each sample for 15, 30, or 60 minutes. Subsequently, the biofilms were stained with crystal violet solution, and the optical density value was measured using a microtiter plate reader at a wavelength of 600 nm. Results: CA and CC were effective in eradicating E. faecalis biofilm. However, the levels of effectiveness of CC and CA depended on the concentration and application time. Three-way analysis of variance (ANOVA) showed a significant difference between the irrigants (p <0.05) and three application times (p <0.05). The CA was effective in eradicating biofilm after 15 minutes of application, whereas the CC was more effective after 30 and 60 minutes of application. Conclusion: CC and CA are both effective in eradicating E. faecalis biofilm.

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A novel and facile synthesis of calcium silicate nanoparticles as a base for root canal cement/sealer under constant potential: Compared to chemical synthesis

Lafmejani,

Parsa,

Mirmohammadi

et al. 2024

Materials Chemistry and Physics

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Efficacy of different instrumentation techniques on reducing Enterococcus faecalis infection in experimentally infected root canals

Cited by 5 publications

References 26 publications

Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics

Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics

Comparing the effectivities of chitosan citrate and chitosan acetate in eradicating Enterococcus faecalis biofilm

A novel and facile synthesis of calcium silicate nanoparticles as a base for root canal cement/sealer under constant potential: Compared to chemical synthesis

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