Efficacy of barriers and hypoxia-inducible factor inhibitors to prevent CO2 pneumoperitoneum-enhanced adhesions in a laparoscopic mouse model

Genipin-crosslinked gelatin microspheres as a strategy to prevent postsurgical peritoneal adhesions: In vitro and in vivo characterization

“…Binda et al reported a significant reduction of adhesions in Balb/c mice, but the research methodology was different from this study since adhesions were laparoscopically induced by hypoxia and normoxia [59], [63], [64] . Efficacy of Hyalobarrier ® was investigated in a rat uterine horn model [65], [66] .…”

Effect of Reteplase™ and PAI-1 antibodies on postoperative adhesion formation in a laparoscopic mouse model

“…using humidified CO 2 insufflation gas to prevent desiccation with the addition of 3% oxygen to prevent hypoxia, and lowering body temperature) and also the application of products which decrease adhesions (i.e. anti-inflammatory agents, calcium-channel blockers, barriers and surfactants [10,11], as well as r-PA, as demonstrated in this study) may be a way to reduce adhesions. This is the third part of our screening experiments in which different agents are being evaluated for their anti-adhesion properties during laparoscopy.…”

“…animals, anaesthesia and ventilation, laparoscopic surgery, induction and scoring of intraperitoneal adhesions, has been described in detail previously [2,3,[6][7][8][9][10][11][12][13][14][15] (Fig. 1).…”

Uncoupling oxidative phosphorylation with 2,4-dinitrophenol promotes development of the adhesion phenotype

“…Hypoxia-inducible factors are heterodimeric transcription factors composed of an oxygen-sensitive a subunit and a constitutively expressed b subunit, which are rapidly degraded under normoxic conditions (18)(19)(20). Hypoxia-inducible factors shift cellular metabolism toward anaerobic metabolism by altering the activity of glycolytic enzymes (11).…”