Genipin-crosslinked gelatin microspheres as a strategy to prevent postsurgical peritoneal adhesions: In vitro and in vivo characterization

Clercq, Kaat De; Schelfhout, Charlotte; Bracke, Marc; Wever, Olivier De; Bockstal, Mieke Van; Ceelen, Wim; Remon, Jean Paul; Vervaet, Chris

doi:10.1016/j.biomaterials.2016.04.012

Cited by 131 publications

(92 citation statements)

References 60 publications

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“…The crosslinking agents can react with the functional groups of gelatin (e.g., –COOH, –NH 2 , –OH, and –CONH–) and form the intramolecular and/or intermolecular gelatin crosspolymers to change the structure of gelatin as well as decrease the thermal and enzymatic degradability (Abd El‐Hady & Albishri, ). Commonly applied crosslinking agents include metal salts, organic acids, genipin, transglutaminase, and dialdehyde carboxymethyl cellulose (De Clercq et al, ; Kaewruang, Benjakul, Prodpran, Encarnacion, & Nalinanon, ; Li, Ye, Li, Li, & Mu, ; Velmurugan, Singam, Jonnalagadda, & Subramanian, ; Wang, Liu, Ye, Wang, & Li, ).…”

Section: Introductionmentioning

confidence: 99%

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Zhu

Zhang

et al. 2019

J Food Biochem

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Gelatin has been identified as a valuable material for controlled release of food ingredients because of its highly ramified three‐dimensional network. In the present work, gelatin was further modified with glutaraldehyde (GA) and prepared as microspheres to further improve its control to release of food ingredients. Features (i.e., chemical structures, thermal properties, network structures, and methylene blue (MB) release kinetics) of both the unmodified and GA‐modified gelatin microspheres were investigated. Results showed that the application of GA enhanced the formation of intramolecular and/or intermolecular crosslinking of gelatin and improved its thermal stability. Besides, the release efficiency of MB from the GA‐modified gelatin was less affected by temperature and pH value compared to the unmodified gelatin. Kinetics analysis proved that the release of MB from gelatin followed the first‐order kinetics. Both the release constant (k) of MB and the diffusion coefficient (Da) of GA‐modified gelatin were significantly lower than those of the unmodified gelatin. Practical applications Gelatin is a valuable material for the release control of food ingredients (e.g., oils, vitamins, antioxidants, and flavor compounds) attributes to its highly ramified three‐dimensional network. However, the low mechanical strength of gelatin makes it susceptible to many environmental factors and has restricted its application. In the present work, gelatin was modified by glutaraldehyde (GA). The chemical structure, thermal properties, network structure, and methylene blue release kinetics of the GA‐modified gelatin microspheres were properly investigated. This research proved that GA‐modified gelatin could be a potential carrier for the release control of given food contents in food industry.

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Section: Introductionmentioning

confidence: 99%

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Zhu

Zhang

et al. 2019

J Food Biochem

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“…The abundance, availability and low cost of gelatin promote its use in a wide variety of applications . Furthermore, gelatins are materials with excellent biocompatibility, biodegradability, non‐toxicity, which possess a great potential as edible films . However, as gelatins are water‐soluble, gelatin modification is required for specific applications in packaging, pharmaceutical and biomedical fields …”

Section: Introductionmentioning

confidence: 99%

Preparation and physicochemistry properties of smart edible films based on gelatin–starch nanoparticles

2018

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“…[38] Surfactants also control the size of nanoparticles by reducing the size and the aggregation tendency of the gelatin droplets during emulsification process. [39] SEM was used to investigate the morphology as well as the particle size of nanoparticles. Nanoparticles displayed a spherical shape with a smooth surface, and no aggregation was observed.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Jain¹

2017

IJGP

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Aim: The aim of this study to evaluates the physiochemical properties, drug loading, in vitro release, and anticancer study. Cancer is a group of diseases characterized by the uncontrolled growth and spread of abnormal cells. If the spread is not controlled, it can result in death. Docetaxel is used to treat primary breast cancer (cancer that started in the breast and has not spread to other parts of the body) in combination with other specific chemotherapy drugs. Docetaxel is also used alone or with other drugs to treat cancer that has spread to areas around the breast such as the lymph nodes above or below the collarbone (known as regional or locally advanced recurrence), or to other parts of the body (secondary breast cancer). Materials and Methods: Docetaxel-loaded gelatin nanoparticles using ultraviolet-visible spectroscopy, X-ray diffraction, particle size and size distribution, scanning electron microscopy, drug entrapment efficiency, differential scanning calorimetry, and energy dispersive X-ray were characterized. Results: Solubility, crystallinity, and the crystal properties of an active pharmaceutical ingredient play a critical role in the value chain of pharmaceutical development, manufacturing, and formulation. The rate of drug release for formulation stored at 45 ± 1°C was increased as compared with the fresh formulation; it might be due to the formation of more pores in the nanoparticles due to evaporation of residual amount of solvent. The tissue distribution studies were performed with docetaxel-loaded gelatin nanoparticles after intravenous (IV) injection in Ehrlich ascites tumor-bearing mice. The tissue distribution studies showed a higher concentration of docetaxel-in the tumor as compared with gelatin nanoparticles. The in vivo tumor inhibition study was also performed after IV injection of docetaxel-loaded gelatin nanoparticles up to 15 days. The docetaxel-loaded gelatin nanoparticles reduced tumor volume significantly as compared with plain docetaxel. Our results revealed that docetaxel-loaded gelatin nanoparticles may perhaps maintain the antioxidant levels and reduce the tumor markers thereby exerting chemopreventive potential. Conclusion: These findings support the use of docetaxel-loaded gelatin nanoparticles in target-specific therapy for cancer treatment.

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Genipin-crosslinked gelatin microspheres as a strategy to prevent postsurgical peritoneal adhesions: In vitro and in vivo characterization

Cited by 131 publications

References 60 publications

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Preparation and physicochemistry properties of smart edible films based on gelatin–starch nanoparticles

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