Efficacy of Antimicrobial and Larvicidal Activities of Green Synthesized Silver Nanoparticles Using Leaf Extract of Plumbago auriculata Lam

Abstract

This work reports the synthesis of silver nanoparticles (AgNPs) using aqueous extract of Plumbago auriculata, and evaluates their antibacterial and larvicidal activities. The synthesized silver nanoparticles were characterized by various spectroscopy techniques, such as FTIR, XRD, TEM, EDX, Zeta potential, and DLS. The synthesized AgNPs exhibited significant antibacterial activity against Gram-positive and Gram-negative bacteria, such as Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae. Furthermore, synthesized nanoparticles inhibited the fourth instars larvae of Aedes aegypti and Culex quinquefasciatus at the concentration of 45.1 and 41.1 µg/mL respectively. Results of dose-dependent studies showed that synthesized nanoparticles were also effective at low concentrations. Molecular docking studies performed with the salivary protein and odorant-binding protein of Aedes aegypti and Culex quinquefasciatus demonstrated that the naphthoquinone compound plumbagin exhibited reliable binding affinity towards the two enzymes. The findings thus reveal that the plant extract and its nanoparticles can be a better alternative to available chemicals to control mosquitos.

Green Synthesis of Silver Nanoparticles Using Diospyros malabarica Fruit Extract and Assessments of Their Antimicrobial, Anticancer and Catalytic Reduction of 4-Nitrophenol (4-NP)

“…The size of the green synthesized AgNPs was found nearly similar between the TEM and DLS analysis. The higher negativity value of the zeta potential specified stability and well-dispersed behavior of nanoparticles [ 70 ]. Thus, our results prove the efficacy of components present in the Diospyros malabarica fruit extract as reducing and capping agents for the synthesis of AgNPs.…”

Green Synthesis of Metal and Metal Oxide Nanoparticles Using Different Plants’ Parts for Antimicrobial Activity and Anticancer Activity: A Review Article

“…Ekennia, Anthony C., et al reported the formation of spherical silver nanoparticles using Euphorbia sanguinea and its photocatalytic degradation of CR (90% within 1 h) [208]. In the period of 2019-2020, several publications reported Silver nanoparticles synthesized using leaf extract of various plants, e.g., Borago officinalis [209], Tragopogon collinus [210], Melia azedarach [211], Mentha aquatica [212], Ziziphus joazeiro [213], Elytraria acaulis [214], Hyptis suaveolens [215], Caesalpinia pulcherrima [216], Gomphrena globosa [217], Plumbago auriculata [218], Cucumis prophetarum [219], Polygonatum graminifolium [220], Cocos nucifera [221], Mimosa albida [222], Capparis zeylanica [223], Holoptelea integrifolia [224], Annona Reticulatal [225], Combretum erythrophyllum [226], Berberis vulgaris [227], Catharanthus roseus [228], Ganonerion polymorphum [229], Premna integrifolia L [230], and Piper betle [231]. Roots are also well established for the synthesis of silver nanoparticles, as shown in Table 13.…”

Nano Zinc Oxide Green-Synthesized from Plumbago auriculata Lam. Alcoholic Extract

“…The produced silver NPs possessed an antioxidant activity and antitumor activity against Dalton's Lymphoma as well as antibacterial activity against Gram-positive and Gram-negative bacteria such as Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia. They also showed larvicidal activity against Aedes aegypti and Culex quinquefasciatus and antitubercular activity against Mycobacterium tuberculosis [3][4][5][6]. Selenium NPs were previously synthesized by green methods using P. zeylanica extract and found to have an antioxidant and antibacterial activities [7].…”