Efficacy of an inactivated whole-cell injection vaccine for nile tilapia, Oreochromis niloticus (L), against multiple isolates of Francisella noatunensis subsp. orientalis from diverse geographical regions

Shahin, Khalid; Shinn, Andrew P.; Metselaar, Matthijs; Ramírez‐Paredes, José Gustavo; Monaghan, Sean J.; Thompson, Kim D.; Hoare, Rowena; Adams, Alexandra

doi:10.1016/j.fsi.2019.03.071

Cited by 27 publications

(22 citation statements)

References 41 publications

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“…Vaccinated fish demonstrated robust upregulation of proinflammatory cytokine and chemokine genes including, IL-1β, TNFα, and CXCL8 compared to the non-vaccinated fish (Figure 2). These findings correspond with recent studies in tilapia, in which a formalin-killed whole cell Fno vaccine enhanced the upregulation of IL-1β and TNFα in the spleen and significantly upregulated IL-1β in the kidney cells of adult zebrafish vaccinated with outer membrane vesicles (OMVs) from Fno at 24 h post-immunization [12,15]. Moreover, the observed marked upregulation of IL-1β was also similar to the early inflammatory response to an experimental Fno infection in tilapia, in which Fno stimulated a significantly higher IL-1β expression in the spleens of Nile tilapia at 24-96 h post-infection [43].…”

Section: Discussionsupporting

confidence: 91%

“…However, with live vaccines, there is always a risk of pathogens reverting to their virulent state. Therefore, an injectable inactivated vaccine with a commercial adjuvant (the oil-based adjuvant Montanide TM ISA 763 AVG) is a better choice against fish bacterial diseases [12,14,22]. Moreover, the efficacy of immersion vaccines is low or moderate when compared to that of injection vaccines.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the emergence of antibiotic-resistant bacterial strains is a severe consequence of the overuse or misuse of antibiotics. Therefore, vaccination is a more effective strategy for controlling and preventing tilapia francisellosis [12][13][14][15]. Currently, approved commercial vaccines against tilapia francisellosis are not available.…”

Section: Introductionmentioning

confidence: 99%

“…This vaccine elicited good protective efficacy (relative percent survival (RPS) 100%) against francisellosis in red Nile tilapia. Shahin et al [12] also evaluated the efficacy of this vaccine against two heterologous Fno strains (from different geographical regions) in Nile tilapia. The results revealed that the vaccinated fish challenged with the homologous isolate showed high protection (an RPS of 82.3%), whereas fish challenged with the two heterologous isolates showed RPSs of 69.8% and 65.9%.…”

Section: Introductionmentioning

confidence: 99%

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Immune Responses and Protective Efficacy of a Formalin-Killed Francisella Noatunensis Subsp. Orientalis Vaccine Evaluated through Intraperitoneal and Immersion Challenge Methods in Oreochromis Niloticus

et al. 2020

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Francisella noatunensis subsp. orientalis (Fno), an intracellular bacterium, causes systemic granulomatous diseases, resulting in high mortality and huge economic losses in Taiwanese tilapia farming. In this study, we tested the efficacy of a formalin-killed Fno vaccine in cultured tilapia. Fno was isolated from diseased tilapia, inactivated with formalin, and mixed with the mineral oil base adjuvant (MontanideTM ISA 763 AVG). A total of 300 tilapia were divided into two groups. The experimental group was intraperitoneally injected with 0.1 mL of vaccine, which was substituted with phosphate-buffered saline (PBS) in the control group. A booster was administered at 2 weeks post-immunization. Tilapia were challenged at 6 weeks post primary immunization by intraperitoneal (IP) injection and immersion methods. Mortality was recorded at 21 and 60 days. The results revealed that the vaccine induced a greater antibody titer and led to 71% and 76% of relative percent survival (RPS) after the IP and immersion challenge. The transcripts of proinflammatory cytokines and immune-related genes, including interleukin-1β (IL-1β), tumor necrosis factor alpha (TNFα), C-X-C motif chemokine ligand 8 (CXCL8), and interleukin-17C (IL-17C), were significantly upregulated after vaccination. Additionally, vaccinated fish had lower bacterial loads in the blood and lower granuloma intensities in the kidney, spleen, liver, and gill than control fish. The results in this study demonstrate that the inactivated Fno vaccine could be an essential resource in Taiwanese tilapia farming.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immune Responses and Protective Efficacy of a Formalin-Killed Francisella Noatunensis Subsp. Orientalis Vaccine Evaluated through Intraperitoneal and Immersion Challenge Methods in Oreochromis Niloticus

et al. 2020

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“…It aids in the prevention of diseases that could pose health risks to the shoal as well as in avoiding the economic losses due to mortality caused by infection. It reduces the contamination of water bodies by the excessive use of antibiotics, and the reduction of final fish product quality [5,24,42,79,100].…”

Section: Introductionmentioning

confidence: 99%

Zebrafish as an alternative animal model in human and animal vaccination research

et al. 2020

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Much of medical research relies on animal models to deepen knowledge of the causes of animal and human diseases, as well as to enable the development of innovative therapies. Despite rodents being the most widely used research model worldwide, in recent decades, the use of the zebrafish (Danio rerio) model has exponentially been adopted among the scientific community. This is because such a small tropical freshwater teleost fish has crucial genetic, anatomical and physiological homology with mammals. Therefore, zebrafish constitutes an excellent experimental model for behavioral, genetic and toxicological studies which unravels the mechanism of various human diseases. Furthermore, it serves well to test new therapeutic agents, such as the safety of new vaccines. The aim of this review was to provide a systematic literature review on the most recent studies carried out on the topic. It presents numerous advantages of this type of animal model in tests of efficacy and safety of both animal and human vaccines, thus highlighting gains in time and cost reduction of research and analyzes.

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Evaluation of Francisella orientalis ΔpdpA as a Live Attenuated Vaccine against Piscine Francisellosis in Nile Tilapia

2022

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Francisella orientalis is an important bacterial pathogen of marine and freshwater fish with worldwide distribution. Fish francisellosis is a severe subacute to chronic granulomatous disease, with high mortalities and high infectivity rates in cultured and wild fish. To date, there is no approved vaccine for this disease. In this study, we evaluated the efficacy of a defined F. orientalis pathogenicity determinant protein A (pdpA) mutant (ΔpdpA) as a live attenuated immersion vaccine against subsequent immersion challenge with the wild-type organism. Immunized Nile tilapia Oreochromis niloticus were protected (45% relative percent survival) from the lethal challenges and presented significantly lower mortality than nonvaccinated and challenged treatments. Although serum IgM was significantly higher in immunized fish, similar bacterial loads were detected in vaccinated and nonvaccinated survivors. In conclusion, although the F. orientalis ΔpdpA is attenuated and effectively stimulated an adaptive immune response, the low relative percent survival and high bacterial persistence in survivors of immunized and challenged treatments indicates low suitability of ΔpdpA as a mucosal vaccine for tilapia under conditions used in this study.

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Efficacy of an inactivated whole-cell injection vaccine for nile tilapia, Oreochromis niloticus (L), against multiple isolates of Francisella noatunensis subsp. orientalis from diverse geographical regions

Abstract: Efficacy of an inactivated whole-cell injection vaccine for nile tilapia, Oreochromis niloticus (L), against multiple isolates of Francisella noatunensis subsp. orientalis from diverse geographical regions.

Cited by 27 publications

References 41 publications

Immune Responses and Protective Efficacy of a Formalin-Killed Francisella Noatunensis Subsp. Orientalis Vaccine Evaluated through Intraperitoneal and Immersion Challenge Methods in Oreochromis Niloticus

Immune Responses and Protective Efficacy of a Formalin-Killed Francisella Noatunensis Subsp. Orientalis Vaccine Evaluated through Intraperitoneal and Immersion Challenge Methods in Oreochromis Niloticus

Zebrafish as an alternative animal model in human and animal vaccination research

Evaluation of Francisella orientalis ΔpdpA as a Live Attenuated Vaccine against Piscine Francisellosis in Nile Tilapia

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