Efficacy of an Extraction Solvent Used to Quantify Albumin Deposition on Hydrogel Contact Lens Materials

Abstract

Although TFA/ACN is efficient at extracting albumin deposited on etafilcon lenses, it does not extract all the albumin that is deposited on SH lenses and alternative extraction procedures should be sought.

Evaluation of Extractants and Precipitants in Tear Film Proteomic Analyses

“…Numerous studies evaluating chemical extractants on conventional hydrogels 11, 13, 14, 19, 31–36 and, most recently, silicone hydrogel lenses, 9, 10, 14, 15, 19, 30, 37–39 have been reported in the scientific literature. The majority of the research has primarily focused on two used in this study: urea buffer 13 and TFA/ACN.…”

Preservation of Human Tear Protein Structure and Function by a Novel Contact Lens Multipurpose Solution Containing Protein-Stabilizing Agents

“…Final SDS concentrations during incubation were 0.02%, 0.04%, 0.08%, and 0.16%, and lactoferrin was used at its normal in vivo concentration (1.7 mg/mL). 1,39 After incubation and native-PAGE, we found that the banding pattern for lactoferrin protein was markedly altered for protein exposed to 0.16% and 0.08% SDS but not at the lower SDS concentrations (Fig. 1A); at lower levels of SDS and in control samples, the protein was observed to produce a single band; after treatment with higher levels of SDS, two bands were observed, running above and below the normal banding position (Fig.…”