Efficacy of acridine orange and papanicolaou stains in sex determination using barr bodies in buccal smears: A comparative study

Aziz, Niloufa Z; Prasad, B Ganesh; Arathi, K; Britto, Frankantony; Aradhya, BV Chethan; Abdulrashid, B

doi:10.4103/inpc.inpc_11_19

Cited by 2 publications

(1 citation statement)

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“…More than or equal to 5% of Barr bodies seen is considered female, and <5% are considered male [24]. In another study, ≤2 Barr bodies seen are male, and >2 Barr bodies are female [19].…”

Section: Discussionmentioning

confidence: 99%

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Koduri¹,

Vataparthi²,

Bolleda³

et al. 2023

Act Sci Oph

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Introduction:Identifying Barr bodies' presence has a significant diagnostic value in multifaceted science disciplines. Testing Barr bodies was important in diagnosing infertility, a syndromic association such as Klinefelter and psychopathic disorders, and disorders of sex development (DSD). It also plays a role in cancer detection in the uterine cervix, identifying transplanted retinal pigment epithelium in porcine models. Identifying the gender of victims or criminals becomes a fundamental requirement in any forensic analysis of a crime scene. The current study hypothesizes identifying the gender using Barr body detection from collected contact lens samples and tries to establish disposed soft contact lenses to consider as evidence found at the crime scene.Methods: A total of 120 (60 males and 60 females) were included in the study; from each subject, contact lens and Buccal samples were collected using sterile wooden toothpicks and soft contact lenses after insertion and removal. Both the buccal and contact lens samples were built into two smears staining with Saffranine and Methylene blue stains. The smears underwent cytological assessment by two examiners using a binocular microscope at 40X. The details of findings were graded on a scale of 1 to 5 based on visualization of Barr bodies seen. Results:The mean rank and median grading scores for higher using saffranine among females across both the samples. The sensitivity is higher at 100% for both the stains among contact lens samples, and specificity is higher among buccal samples, 93% for saffranine and 90% for methylene blue. Conclusion:Overall, it is conspicuous that contact lenses can be considered as evidence found at the crime scene in identifying the gender using Barr body detection.

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“…More than or equal to 5% of Barr bodies seen is considered female, and <5% are considered male [24]. In another study, ≤2 Barr bodies seen are male, and >2 Barr bodies are female [19].…”

Section: Discussionmentioning

confidence: 99%

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Koduri¹,

Vataparthi²,

Bolleda³

et al. 2023

Act Sci Oph

View full text Add to dashboard Cite

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Comparative cytopathologic evaluation using acridine orange with Papanicolaou stain in psychoactive substances abusers with potentially malignant and malignant disorders

Patel

Bhavasar

Shah

et al. 2021

J Oral Maxillofac Pathol

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Introduction: Oral cancer is a major health problem and its early detection is advantageous for therapeutic purposes. According to available evidence, the risks of oral malignancies increase with the usage of tobacco and other psychoactive substances (PSs). The present study showed expression pattern of nuclear and cytoplasmic changes from normal individuals without habit to oral potentially malignant disorders (OPMD) and oral squamous cell carcinoma (OSCC) in PS abusers with the help of fluorescence acridine orange (AO) stain and Papanicolaou (PAP) stain. Aim and Objectives: This study aimed to investigate and compare diagnostic efficacy of fluorescence microscopic evaluation of AO stain in cytological smears with PAP staining under light microscopy in PS abusers having oral potentially malignant and malignant lesions. Materials and Methods: Oral smears from 120 individuals among which 40 from potentially malignant disorders, 40 from oral malignancy and 40 normal buccal mucosa smears were prepared. One set of smears was stained by AO staining and the other by PAP staining and examined under fluorescence and light microscope, respectively. The results of both the stainings were evaluated by grading cytology smears in class-I to class-V cytology. Results: The AO fluorescence stain reliably demonstrated malignant cells based on the differential fluorescence. The efficacy of AO fluorescence stain was higher than PAP stain in screening of oral lesions suspicious of malignancy. The sensitivity of PAP staining and AO staining is 57.50% and 61.25%, respectively. Conclusion: As compared to PAP staining method, fluorescent AO method is more effective in screening of OPMD and OSCC in PS abusers.

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Efficacy of acridine orange and papanicolaou stains in sex determination using barr bodies in buccal smears: A comparative study

Cited by 2 publications

References 0 publications

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Comparative cytopathologic evaluation using acridine orange with Papanicolaou stain in psychoactive substances abusers with potentially malignant and malignant disorders

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