Efficacy of a porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) natural recombinant against a heterologous PRRSV-1 isolate both clustered within the subgroup of BJEU06-1-like isolates

Li, Chen; Li, Shuai; Li, Shubin; Qiu, Ming; Hong, Lin; Sun, Zhe; Qiu, Yuejia; Qi, Wenhao; Feng, Binghui; Li, Jixiang; Zheng, Wanglong; Yu, Xiaofang; Tian, Kegong; Shang, Shaobin; Fan, Kewei; Zhu, Jianzhong; Chen, Nanhua

doi:10.1016/j.vetmic.2023.109847

Cited by 4 publications

(7 citation statements)

References 39 publications

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“…The parental HLJB1 isolate has been determined to have low virulence [ 21 ]. In this study, we evaluated the pathogenicity of rHLJB1 and ORF2-6-CON in piglets.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have confirmed that Chinese PRRSV1 wild-type (WT) isolates are pathogenic to pigs [ 8 , 10 , 11 , 15 ]. Our previous study showed that the low-virulence HLJB1 isolate cannot provide satisfactory cross-protection against the heterologous SD1291 isolate [ 21 ]. Therefore, we evaluated the cross-protective efficacy of a PRRSV1 ORF2-6 chimeric virus (ORF2-6-CON) in this study.…”

Section: Discussionmentioning

confidence: 99%

“…To detect porcine Tfh cells, fresh mononuclear cells were seeded into 96-well V-bottom plates (2 × 10 6 /well). Porcine Tfh cells were stained as previously described [ 21 , 37 , 38 ]. To evaluate the dynamics of IFNγ-secreting T lymphocytes, 2 × 10 6 cells were seeded in 96-U-bottom-well plates and stimulated with the PRRSV1 heterologous SD1291 strain (multiplicity of infection, MOI = 0.1) for 20 h, while uninfected cells cultured in RPMI-1640 medium were used as a negative control.…”

Section: Methodsmentioning

confidence: 99%

“…During the last 5 h of incubation, brefeldin A (Sigma‒Aldrich, USA) was added to the cultures at a final concentration of 5 µg/mL. Subsequently, porcine IFNγ-secreting T cells were stained as we described previously [ 21 ]. The stained porcine Tfh cells and IFNγ-secreting T cells were resuspended for FACS analysis.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, minor envelope proteins encoded by ORF2-4 genes and major envelope proteins encoded by ORF5-6 genes play synergistic roles in conferring cross-protection [ 20 ]. A recent study determined that the low-virulence HLJB1 isolate could provide limited cross-protection against only the heterologous SD1291 isolate even though they were both clustered within the PRRSV1 BJEU06-1-like subgroup [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection

Li,

Qiu,

et al. 2024

Vet Res

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The prevalence of porcine reproductive and respiratory syndrome virus 1 (PRRSV1) isolates has continued to increase in Chinese swine herds in recent years. However, no effective control strategy is available for PRRSV1 infection in China. In this study, we generated the first infectious cDNA clone (rHLJB1) of a Chinese PRRSV1 isolate and subsequently used it as a backbone to construct an ORF2-6 chimeric virus (ORF2-6-CON). This virus contained a synthesized consensus sequence of the PRRSV1 ORF2-6 gene encoding all the envelope proteins. The ORF2-6 consensus sequence shared > 90% nucleotide similarity with four representative strains (Amervac, BJEU06-1, HKEU16 and NMEU09-1) of PRRSV1 in China. ORF2-6-CON had replication efficacy similar to that of the backbone rHLJB1 virus in primary alveolar macrophages (PAMs) and exhibited cell tropism in Marc-145 cells. Piglet inoculation and challenge studies indicated that ORF2-6-CON is not pathogenic to piglets and can induce enhanced cross-protection against a heterologous SD1291 isolate. Notably, ORF2-6-CON inoculation induced higher levels of heterologous neutralizing antibodies (nAbs) against SD1291 than rHLJB1 inoculation, which was concurrent with a higher percentage of T follicular helper (Tfh) cells in tracheobronchial lymph nodes (TBLNs), providing the first clue that porcine Tfh cells are correlated with heterologous PRRSV nAb responses. The number of SD1291-strain-specific IFNγ-secreting cells was similar in ORF2-6-CON-inoculated and rHLJB1-inoculated pigs. Overall, our findings support that the Marc-145-adapted ORF2-6-CON can trigger Tfh cell and heterologous nAb responses to confer improved cross-protection and may serve as a candidate strain for the development of a cross-protective PRRSV1 vaccine.

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“…The parental HLJB1 isolate has been determined to have low virulence [ 21 ]. In this study, we evaluated the pathogenicity of rHLJB1 and ORF2-6-CON in piglets.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection

Li,

Qiu,

et al. 2024

Vet Res

Self Cite

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Characterization and Pathogenicity of the Novel Porcine Reproductive and Respiratory Syndrome Virus 1 Strain SL-01 in China

Zheng,

Wu,

Gao

et al. 2024

Transboundary and Emerging Diseases

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Currently, PRRSV-1 causes a large number of clinical infections in Chinese swine herds, and the prevalence of new strains has presented great challenges. In this study, the novel PRRSV-1 strain SL-01 was isolated, with a genome length of 14,978 bp, and genetic evolution analysis revealed that it belonged to a new subtype branch. Sequence homology analysis showed that the strain was only 82.2%–86.7% identical to the current classical PRRSV-1 strains. In particular, the novel strain exhibited a unique deletion pattern in Nsp2. In addition, GP3 and GP4 of the SL-01 strain showed four consecutive amino acid deletions in the highly variable regions at amino acids 243–248 and 63–68, respectively. Further challenges in piglet and pregnant sow demonstrated that the SL-01 strain could cause the piglet fever and death but less pathogenic to pregnant sow. Overall, the characterization and pathogenicity of a novel PRRSV-1 strain were first explored and provide a prevention for pig farms.

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Integrated microbiome and metabolome analysis reveals that new insight into Radix pseudostellariae polysaccharide enhances PRRSV inactivated vaccine

Xu,

You,

Zheng

et al. 2024

Front. Immunol.

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In this study, we investigated how Radix pseudostellariae polysaccharide (RPP) enhances the immune response of the inactivated porcine reproductive and respiratory syndrome virus (PRRSV) vaccine through interactions with the microbiome and metabolome. We pretreated sows with 10 mg/kg body weight of RPP via drinking water for 7 days prior to intramuscular injection of the PRRSV vaccine. This significantly increased the concentrations of PRRSV GP5 protein antibody, interleukin (IL)-2, IL-4, IL-10, and interferon (IFN)-γ. Oral administration of RPP also significantly improved the abundance of beneficial bacteria in the stool, such as Parabacteroides distasonis, Prevotella_copri, Eubacterium_sp., and Clostridium_sp._CAG:226, and decreased the levels of potentially pathogenic bacteria, such as Paraeggerthella and [Clostridium] innocuum, compared to the vaccine alone. These bacterial changes were confirmed using quantitative real-time polymerase chain reaction (Q-PCR). Moreover, RPP treatment significantly increased the blood concentrations of L-theanine, taurodeoxycholic acid (TDCA), and N-arachidonoyl proline, and decreased the levels of L-glutamine, oclacitinib, lipoxin C4, and leukotriene C5 in sows after immunization (p< 0.05). The concentrations of various blood metabolites were validated using sandwich enzyme-linked immunosorbent assay (ELISA), confirming the accuracy of the metabolomics data. Intriguingly, the integration of microbiome and metabolome analyses highlighted the significance of Prevotella_copri and TDCA. We consequently developed a mouse immunity model using GP5 protein and discovered that oral administration of RPP significantly enhanced the levels of GP5 protein antibodies, IL-2, IL-4, IL-10, and IFN-γ in mouse serum. It also increased the number of CD3+ and CD3+CD4+ cells in the spleen. Additionally, Prevotella_copri was administered into the large intestine via the anus for 7 days prior to the intramuscular injection of the PRRSV GP5 protein. The results demonstrated a significant increase in TDCA and GP5 antibody concentration in the mouse serum, indicating that RPP modulates Prevotella_copri to elevate its metabolite TDCA, thereby enhancing the GP5 antibody level. In conclusion, oral administration of 10 mg/kg RPP optimizes gut flora diversity and blood metabolites, particularly Prevotella_copri and TDCA, thereby improving the immune response to the inactivated PRRSV vaccine.

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Efficacy of a porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) natural recombinant against a heterologous PRRSV-1 isolate both clustered within the subgroup of BJEU06-1-like isolates

Cited by 4 publications

References 39 publications

A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection

A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection

Characterization and Pathogenicity of the Novel Porcine Reproductive and Respiratory Syndrome Virus 1 Strain SL-01 in China

Integrated microbiome and metabolome analysis reveals that new insight into Radix pseudostellariae polysaccharide enhances PRRSV inactivated vaccine

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