Efficacy of a new combined larvicidal–adulticidal ultralow volume formulation against Aedes aegypti (Diptera: Culicidae), vector of dengue

Abstract

A new ultralow volume formulation (ULV) containing permethrin as an adulticidal active ingredient and the insect growth regulator (IGR) pyriproxyfen as a larvicide was developed and its efficacy evaluated in a field trial in Wanda, Misiones (Argentina). Two separate study areas were sprayed: one with a ULV formulation of permethrin 15% plus pyriproxyfen 3% and the other with permethrin 15% only. A third untreated area was kept as a control. Sentinel cages containing adult mosquitoes and jars containing Aedes aegypti larvae III/IV were placed in treated and control areas. The residual activity of the formulations was tested using 20 L water containers. The adulticide effect of permethrin 15% + pyriproxyfen 3% formulation was similar to the permethrin 15% formulation, however, the inhibition of adult emergence in the treatment with permethrin 15% never exceeded 20%, whereas the inhibition of adult emergence in the treatment with permethrin 15% plus pyriproxyfen 3% showed initial values of 96% maintaining this high level of inhibition up to 35 days after ULV spraying. Larval indexes (House and Breteau indexes) showed that a greater, long-lasting effect was obtained with the permethrin 15% plus pyriproxyfen 3% formulation.

Biochemical and molecular analyses to determine pyrethroid resistance in Aedes aegypti

“…The rotational use of different classes of insecticides is becoming an important one in the fight against spreading insecticide resistance. In addition, the combination of synergists, such as piperobyl butoxide (PBO), a well-know inhibition of microsomal monooxygenase, with larvicides and/or adulticides has successfully identified to increase the efficiency of current vector control [52][53][54]. …”

Multiple insecticide resistance in Aedes aegypti (Diptera: Culicidae) populations compromises the effectiveness of dengue vector control in French Guiana

“…Therefore, control based only on community action may not be easy to achieve. Recent work has successfully identified methods to increase the efficiency of current vector control by using a mixture of larvicides and/or adulticides and/or synergists (Darriet & Corbel 2006, lucia et al 2009), treating at different times during the year (Chung et al 2001(Chung et al , lothrop et al 2008 or using geographic information system technology to optimise spraying operations (Mak et al 2007). Pull-to-kill strategies (Perich et al 2003, Williams et al 2007) also produced promising results by reducing Aedes spp density while targeting them specifically.…”

A New Strategy for Aedes aegypti (Diptera: Culicidae) Control With Community Participation Using a New Fumigant Formulation

“…A new smoke-generating formulation also containing pyriproxyfen and permethrin was evaluated under laboratory conditions against Ae. aegypti with the idea of Þnding an alternative to conventional tools for indoor treatments (Harburguer et al 2009). …”

“…The development of a novel insecticidal formulation containing pyriproxyfen as a larvicide and permethrin as an adulticide that is effective against both adult and immature aquatic forms of mosquitoes signiÞcantly increased Ae. aegypti control in recent Þeld trials carried out in Argentina (Masuh 2008, Lucia et al 2009). A new smoke-generating formulation also containing pyriproxyfen and permethrin was evaluated under laboratory conditions against Ae.…”