Biochemical and molecular analyses to determine pyrethroid resistance in Aedes aegypti

Lin, Yousheng; Tsen, Wei-Lun; Tien, Nai-Yueh; Luo, Yi-Pey

doi:10.1016/j.pestbp.2013.08.004

Cited by 21 publications

(23 citation statements)

References 46 publications

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“…They observed that resistance to transfluthrin was less pronounced than that observed to metofluthrin. 32 This result is similar to the lower repellency resistance observed in the PR strain to transfluthrin compared to metofluthrin observed in the present study. In contrast to these findings, a recent paper by Bowman et al 22 found that a Puerto Rico strain of Ae.…”

Section: Discussionsupporting

confidence: 90%

“…demonstrated differences between transfluthrin and metofluthrin in KT 50 values of two kdr alleles screened in Aedes aegypti , V1023G and D1794Y. They observed that resistance to transfluthrin was less pronounced than that observed to metofluthrin . This result is similar to the lower repellency resistance observed in the PR strain to transfluthrin compared to metofluthrin observed in the present study.…”

Section: Discussionsupporting

confidence: 87%

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Reduced effectiveness of repellents in a pyrethroid‐resistant strain of Aedes aegypti (Diptera: culicidae) and its correlation with olfactory sensitivity

Liu

Norris

Jiang

et al. 2019

Pest Management Science

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BACKGROUND The mosquito, Aedes aegypti (Diptera: Culicidae), is a vector of dengue fever, zika, chikungunya, and yellow fever viruses, and in many areas possesses significant levels of resistance to pyrethroids. Behavioral performance was assessed in 15, 30, and 60 min exposures in a high throughput vapor phase spatial repellency assay to three contact repellent standards: N,N‐diethyl‐3‐methylbenzamide (DEET), ethyl 3‐[acetyl(butyl)amino] propanoate (IR3535), and 2‐undecanone, as well as pyrethrum extract, transfluthrin, and metofluthrin in susceptible (Orlando) and a pyrethroid‐resistant Puerto Rico strain of Aedes aegypti. Additionally, electroantennographic studies were used to investigate the antennal sensitivities to these compounds in both strains. RESULTS Resistance was found to all tested insect repellents in the Puerto Rico strain of Ae. aegypti. Resistance ratios at the different time points were about 2 for DEET, 3 for 2‐undecanone, and 12 for IR3535. Resistance was also observed to pyrethrum extract (∼9‐fold), transfluthrin (∼5‐fold), and metofluthrin (∼48‐fold) in repellent behavioral response. Electrophysiological analysis found decreased antennal sensitivity to all repellents tested, consistent with their behavioral effects. CONCLUSION The reduced sensitivity to these repellents may represent a fitness cost arising from the kdr mutation present in Puerto Rico Aedes aegypti. This work highlights the need for understanding collateral effects from the evolution of pesticide resistance in mosquitoes, and the importance of finding alternative strategies to control resistance development. © 2019 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 87%

Reduced effectiveness of repellents in a pyrethroid‐resistant strain of Aedes aegypti (Diptera: culicidae) and its correlation with olfactory sensitivity

Liu

Norris

Jiang

et al. 2019

Pest Management Science

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“…Se ha considerado que las enzimas detoxificadoras más importantes son: glutatión S-transferasas (GSTs), monooxigenasas (citocromos P-450), oxidasas de función mixta (FOM) y esterasas (Bisset, 2002;Ranson et al, 2002;Díaz et al, 2004;Badii y Garza, 2007;Hsu et al, 2011;Liu, 2012;Ying et al, 2013;French et al, 2013).…”

Section: Resistencia Metabólicaunclassified

“…Diverse works correlate the resistance to pesticides with the increase of enzymatic activity, attributing it to punctual mutations, genic duplications and mutations in the target molecule (Mutero et al, 1994;Villatte et al, 2000;Vontas et al, 2001;Vontas et al, 2002;Ortelli et al, 2003;Menozzi et al, 2004;Revuelta et al, 2009;Ying et al, 2013;Chang et al, 2014;Zhao et al, 2014;Xi et al, 2015).…”

Section: Metabolic Resistancementioning

confidence: 99%

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Rojas

Bermudez

Cambero-Campos

et al. 2017

rbio

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“…An additional mutation (D1794Y), which appears to have a similar effect to S989P when found in conjunction with V1016G, is known from Ae. aegypti in Taiwan (Chang, Huang, Chang, Wu, & Dai, 2012;Chang et al, 2009;Lin, Tsen, Tien, & Luo, 2013). Smith et al (2016) summarised the findings of multiple studies on the combinations of mutations that lead to pyrethroid resistance as follows: the V1016G mutation found alone confers resistance to Type I (those without an α-cyano group) and Type II pyrethroids (those with an α-cyano-3-phenoxybenzyl group) whereas F1534C alone confers resistance only to pyrethroids of Type I and to DDT (Du, Nomura, Zhorov, & Dong, 2016).…”

Section: Introductionmentioning

confidence: 99%

Heterogeneous genetic invasions of three insecticide resistance mutations in Indo-Pacific populations ofAedes aegypti(L.)

Endersby‐Harshman

Schmidt

Chung

et al. 2019

Preprint

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AbstractNations throughout the Indo-Pacific region use pyrethroid insecticides to control Aedes aegypti, the mosquito vector of dengue, often without knowledge of pyrethroid resistance status of the pest or origin of resistance. Two mutations (V1016G + F1534C) in the sodium channel gene (Vssc) of Ae. aegypti modify ion channel function and cause target-site resistance to pyrethroid insecticides, with a third mutation (S989P) having a potential additive effect. Of 27 possible genotypes involving these mutations, some allelic combinations are never seen while others predominate. Here, five allelic combinations common in Ae. aegypti from the Indo-Pacific region are described and their geographical distributions investigated using genome-wide SNP markers. We tested the hypothesis that resistance allele combinations evolved de novo in populations, versus the alternative that dispersal of Ae. aegypti between populations facilitated genetic invasions of allele combinations. We used latent factor mixed-models to detect SNPs throughout the genome that showed structuring in line with resistance allele combinations and compared variation at SNPs within the Vssc gene with genome-wide variation. Mixed-models detected an array of SNPs linked to resistance allele combinations, all located within or in close proximity to the Vssc gene. Variation at SNPs within the Vssc gene was structured by resistance profile, while genome-wide SNPs were structured by population. These results demonstrate that alleles near to resistance mutations have been transferred between populations via linked selection. This indicates that genetic invasions have contributed to the widespread occurrence of Vssc allele combinations in Ae. aegypti in the Indo-Pacific region, pointing to undocumented mosquito invasions between countries.

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Biochemical and molecular analyses to determine pyrethroid resistance in Aedes aegypti

Cited by 21 publications

References 46 publications

Reduced effectiveness of repellents in a pyrethroid‐resistant strain of Aedes aegypti (Diptera: culicidae) and its correlation with olfactory sensitivity

Reduced effectiveness of repellents in a pyrethroid‐resistant strain of Aedes aegypti (Diptera: culicidae) and its correlation with olfactory sensitivity

Untitled

Heterogeneous genetic invasions of three insecticide resistance mutations in Indo-Pacific populations ofAedes aegypti(L.)

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