Efficacy of a natural coagulant protein from Moringa oleifera (Lam) seeds in treatment of Opa reservoir water, Ile-Ife, Nigeria

Abstract

Health related and environmental side effects associated with conventional chemical coagulants used in water treatment has prompted the search for natural alternatives, especially of plant origin. This study investigated the water coagulation activities of a purified protein from Moringa oleifera seeds on the water from Opa reservoir of Obafemi Awolowo University, Ile-Ife. M. oleifera coagulant protein (MoCP) was purified via ion exchange and gel filtration chromatography respectively. Subunit and native molecular weight as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration was 14.2 kDa and 30.3 kDa respectively. Modified jar test was used to investigate the coagulation activity of the purified protein in comparison to that of conventional chemical coagulant (aluminium sulphate). MoCP significantly reduced turbidity (p < 0.05) and organic load which contributed to about 58.18% reduction in total coliform of treated water. MoCP also elicited promising antimicrobial activity against bacterial isolates in the water from Opa reservoir.

In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in Brassica juncea and Moringa oleifera

“…The peptides that were identified in the low molecular weight peptide profiling and napin profiling are displayed in Supplmentary Materials Table S1 and Supplmentary Materials Figure S1a . In total, 2174 peptide in MOP ( Supplmentary Materials Table S1 ) and BJP 1119 peptide ( Supplmentary Materials Table S2 and Supplmentary Materials Figure S1b ) were notorious and were eluted and tested in vitro for antimicrobial activity; the peptide sequences were screened using an in silico platform [ 10 ], peptide profiling was developed in QS 3.0 software (Applied Biosystems, Seoul, Korea) to predict potential antimicrobial peptides (AMPs). Although many potential antimicrobial peptides were identified, the peptides KAVKQQIQQQGQQQGKQQMVSR, FFFLLTN, PPLLQQCCNEL, SGGGPS, and GPQQRPPLLQQCCNELHQEEP ( Supplmentary Materials Figure S1 ) were the most abundant.…”

“…Moringa ( Moringa oleifera ) is one of the widely cultivated species in the tropical region of Asia, Africa, and South America, and it is used in rural areas of Africa for water treatment [ 8 ]. The previous study indicates that various parts of Moringa such as leaves, roots, and bark have antibacterial activity, and the seeds are well known for their coagulant and antibacterial properties [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. The seed extract and recombinant protein of M. oleifera are effective against Staphylococcus aureus , Streptococcus pyrogenes , Streptococcus mitis , Streptococcus pneumoniae , Enterococcus faecalis , Escherichia coli, and Legionella pneumophila for up to 150 min [ 15 ].…”

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

“…Moringa oleifera Lam (Family: Moringaceae ), is a primordial northeastern India fast-growing softwood tree distributed worldwide and possesses diverse pharmacological potentials [21] . M. oleifera is a well-known plant in Nigeria, where it is being exploited for different purposes [ 22 , 23 ]. Isolation of lectins has been reported from distinct parts of the M. oleifera plant, with diverse activities attributed to these quintessential proteins [24] , [25] , [26] .…”

The Effect of the Operational Parameters on Turbidity Removal Using Peanut Cake as Primary Coagulant

“…Availability of potable water witnessed diminishing recently due to the current trends in development, which resulted in upsurge pollution as a result of growing economic activities, contributing to the annual death exceeded 2 million children under age 5 from water-borne and pneumonia related disease [1]. Rural communities of developing countries are more experiencing the lake of water due to insufficient water and wastewater treatment facilities [2,3].…”