Efficacy of a high free iodine barrier teat disinfectant for the prevention of naturally occurring new intramammary infections and clinical mastitis in dairy cows

Abstract

Using a natural exposure trial design, the goal of our study was to evaluate the clinical efficacy of an iodine teat disinfectant with barrier properties and a high level of free iodine relative to a conventional iodine teat disinfectant with no barrier properties and low levels of free iodine. During the 18 wk of the trial, quarter milk samples were collected every 2 wk from 385 dairy cows from 2 herds. Cows on both farms were assigned in a balanced way according to milk yield, number of lactation, days in milk, somatic cell count (SCC) and microbiology culture pretrial into one of following groups: nonbarrier post milking teat disinfectant (NBAR; n = 195 cows; 747 quarters) or barrier postmilking teat disinfectant (BAR; n = 190 cows; 728 quarters). Afterward, at each scoring date every 2 wk, milk SCC was quantified in samples from all mammary quarters and microbiologic culture was only performed on milk samples with SCC >200,000 cells/mL for multiparous cows and SCC >100,000 cells/mL for primiparous cows. A new intramammary infection (NIMI) was defined when a quarter had milk SCC <200,000 cells/mL for multiparous cows and <100,000 cells/mL for primiparous without microorganism isolation, and in a subsequent sampling visit had milk SCC >200,000 cells/mL for multiparous cows and >100,000 cells/mL for primiparous cows, and positive microorganism isolation. A quarter could have several NIMI, but only 1 case per specific pathogen was considered. The most frequently isolated microorganism group on both farms was Streptococcus spp. (6.25% of total mammary quarters), followed by coagulase-negative staphylococci (3.6%) and Corynebacterium spp. (1.5%). In the present study, an interaction occurred between treatment and week of trial on the incidence risk of NIMI. Quarters disinfected with BAR had 54 and 37% lower odds of NIMI than quarters disinfected with NBAR at 8 and 16 wk of the trial, respectively; whereas at other weeks of the study both products had similar incidence risks of NIMI. Overall, teats disinfected with BAR had 46% lower odds of acquiring a clinical mastitis than those disinfected with NBAR. We concluded that the postmilking teat disinfectant with barrier properties and higher free iodine content reduced the risk of clinical mastitis, although differences in new infections were detected at only weekly time points.

Morphological changes in cows’ mammary gland tissue under chronic form of mastitis

“…Це і клінічне обстеження загального стану тварини, й органолептичне, фізико-хімічне, бактеріологічне дослідження секрету молочної залози, рентгенологічне, УЗД, лазерна, гістологічна, цитологічна діагностика та інш. (Kharuta et al, 1998;Dmytriv, 2002;Kasianchuk, 2002;Grohn et al, 2004;Martins et al, 2017;Blum et al, 2017;Horiuk et al, 2018).…”

Estudio en el Ecuador de la curva de calibración para el conteo total de bacterias por citometría de flujo de leche cruda bovina

“…Los estudios realizados por Cassoli, (2007) indicaron la importancia de considerar la citometría de flujo como método alternativo para el conteo de bacterias en leche y la determinación de la calidad higiénica en la leche cruda antes de su comercialización. Un conteo elevado de microorganismos mesó-filos aeróbicos puede estar relacionado con diferentes fuentes de contaminación al momento del ordeño y transporte de la leche, así como con variaciones en la temperatura de almacenamiento (Matsubara, 2011;Martins, 2017). La normativa ecuatoriana para mesófilos aerobios define un límite máximo de 1,5 millones de UFC/mL; sin embargo, las regulaciones industriales penalizan al productor en el pago por litro de leche para conteos superiores a 300000 UFC/mL.…”

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“…Los estudios realizados por Cassoli, (2007) indicaron la importancia de considerar la citometría de flujo como método alternativo para el conteo de bacterias en leche y la determinación de la calidad higiénica en la leche cruda antes de su comercialización. Un conteo elevado de microorganismos mesó-filos aeróbicos puede estar relacionado con diferentes fuentes de contaminación al momento del ordeño y transporte de la leche, así como con variaciones en la temperatura de almacenamiento (Matsubara, 2011;Martins, 2017…”