Efficacy of a combined nanoparticulate/calcium hydroxide root canal medication on elimination of <i><scp>E</scp>nterococcus faecalis</i>

Javidi, Maryam; Afkhami, Farzaneh; Zarei, Mina; Ghazvini, Kiarash; Rajabi, Omid

doi:10.1111/aej.12028

Cited by 82 publications

(82 citation statements)

References 28 publications

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“…In addition, a suspension of AgNP at a concentration of 100 μg/mL favors the transportation of calcium hydroxide inside the root canal increasing its effectiveness against E. faecalis biofilm (5). The combination between calcium hydroxide and 200 μg/mL AgNP (sized in 70 nm) was also effective eliminating E. faecalis after only 1 day of contact (34).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

Rodríguez

Ramírez-Mora

Montero

et al. 2016

Odovtos - Int J Dent Sc

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The purpose of this study was to measure the antibacterial efficacy of a dispersion of silver nanoparticles (AgNP) in a citrate medium tested in two E. faecalis strains. AgNP were synthesized, and AgNP citrate medium (AgNP-CM) dispersion was prepared at a concentration of 100 μg/mL. The antibacterial efficacy of AgNP-CM dispersion was evaluated over two E. faecalis strains: ATCC29212 and a wild strain collected from human necrotic teeth. 5% sodium hypochlorite (NaOCl) and sterile saline solution were used as positive and negative controls. 5 and 30-minute contact tests were conducted and each experimental group were replicated 10 times. After 24 hours of incubation, the Log CFU/ mL were calculated. The AgNP obtained showed spherical shapes and had 30-60nm size. 5% NaOCl was able to completely eliminate both E. faecalis strains in all groups, showing a significant statistical difference when compared to AgNP-CM dispersion and negative control groups. AgNP-CM dispersion showed a statistically significant decrease in Log CFU/mL averages (p=0,0006) when compared to the sterile saline solution for the ATCC29212 strain during the 30-minute time. Between the 5-minute and 30-minute groups, a significant bacterial count decrease was also observed (p= 0,0128). The antibacterial efficacy of the dispersion was greater for the ATCC29212 strain than the wild strain, were the effect diminished. AgNP-CM dispersion showed a significantly lower antibacterial efficacy against E. faecalis than the 5% NaOCl at the tested times. RESUMENEl propósito del presente estudio fue medir la eficacia antibacteriana de una dispersión de nanopartículas de plata (AgNP) en medio de citrato ante dos cepas de E. faecalis. Las AgNP fueron sintetizadas y se preparó una dispersión en medio de citrato (AgNP-CM) en una concentración de 100 μg/mL. La eficacia antibacteriana de la dispersión de AgNP-CM fue evaluada ante dos cepas de E. faecalis: la ATCC29212 pura y una cepa silvestre aislada de un tratamiento endodóntico fallido. Se utilizó hipoclorito de sodio al 5% de concentración (NaOCl) y solución salina estéril como controles positivos y negativos. Se realizaron pruebas de contacto durante 5 y 30 minutos y cada grupo experimental se replicó 10 veces. Después de incubar las muestras durante 24 horas, se calcularon las concentraciones Log CFU/mL. Las AgNP obtenidas mostraron formas esféricas y un tamaño entre los 30 y 60nm. El NaOCl 5% logró eliminar por completo las cepas bacterianas en todos los grupos, mostrando una diferencia estadísticamente significativa al compararse con las dispersiones de AgNP-CM y los controles negativos. La dispersión de AgNP-CM mostró una disminución estadísticamente significativa en los promedios Log CFU/mL al compararse con la solución salina estéril para la cepa ATCC29212 a los 30 minutos de contacto. Entre los grupos de 5 y 30 minutos de la dispersión, se observó también una disminución en el conteo bacteriano (p=0,0128). La eficacia antibacteriana de la dispersión fue mayor para la cepa ATCC29212 que para la c...

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Section: Discussionmentioning

confidence: 99%

Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

Rodríguez

Ramírez-Mora

Montero

et al. 2016

Odovtos - Int J Dent Sc

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“…Enterococcus faecalis may survive after root canal procedures, contributing to endodontic treatment failure 1 . Calcium hydroxide is used as medication in teeth with pulp necrosis, promoting microorganism reduction in the RCS, favoring the endodontic treatment success 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Calcium hydroxide is used as medication in teeth with pulp necrosis, promoting microorganism reduction in the RCS, favoring the endodontic treatment success 2 . Calcium hydroxide (CH) has antimicrobial effect, due to its dissociation into Ca + and OH -ions, promoting an alkaline pH 1,3 and interaction with the microorganism cell wall, and inactivating bacterial byproducts such as endotoxin [4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

“…The search for a better antimicrobial effectiveness of CH has been intense and several substances have been associated 1,2,4,7,8 . Association of CH with chlorhexidine is indicated in primary and secondary endodontic infections, increasing antimicrobial activity against microorganisms resistant to calcium hydroxide, such as E. faecalis and C. albicans 9-11 .…”

Section: Introductionmentioning

confidence: 99%

“…Nanoparticle substances may be used in the composition of intracanal medications and endodontic sealers, increasing antibacterial activity, due they present higher active surface area 1 . The incorporation of nanoparticles zinc oxide to CH can improve their radiopacity, consistency and antimicrobial activity without interferer with their other properties, such as hydroxyl ions release.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial activity of intracanal medications based on calcium hydroxide and zinc oxide micro- or nanoparticles: an ex vivo study

Guerreiro-Tanomaru

Aguiar

Chávez-Andrade

et al. 2017

Rev. odontol. UNESP

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Introduction Nanoparticles and associations to calcium hydroxide can be used to increase antimicrobial activity. Objective To evaluate antibacterial activity against Enterococcus faecalis and pH of intracanal medications (IM) based on calcium hydroxide (CH) and zinc oxide (ZnO) micro- or nanoparticles, and their association with 0.4% chlorhexidine (CHX). Material and method Root canals from single-rooted human teeth were inoculated and incubated for 21 days. After sample (S1), the root canals were filled with the medications for 7 days and samples were collected immediately after medication (S2) and 7 days later (S3). Counting of CFU mL-1 was performed. Polyethylene tubes filled with the medications were used for the pH evaluation after 3, 7, 14 and 28 days. Data were submitted to ANOVA and Tukey tests (p<0.05). Result All IM promoted elimination of E. faecalis immediately after medication (S2). All the pastes promoted a similar pH increase. Conclusion CH/ZnO micro- or nanoparticles associated with CHX promoted greater bacterial reduction in the root canals and similar pH.

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Advances in Materials and Techniques for Microbial Control

Zapata¹,

Arias-Moliz²,

Neelakantan³

et al. 2022

Endodontic Advances and Evidence‐Based Clinical Guidelines

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Efficacy of a combined nanoparticulate/calcium hydroxide root canal medication on elimination of Enterococcus faecalis

Cited by 82 publications

References 28 publications

Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

Antibacterial activity of intracanal medications based on calcium hydroxide and zinc oxide micro- or nanoparticles: an ex vivo study

Advances in Materials and Techniques for Microbial Control

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