Efficacy assessment of commercially available natural products and antibiotics, commonly used for mitigation of pathogenic Vibrio outbreaks in Ecuadorian Penaeus (Litopenaeus) vannamei hatcheries

Sotomayor, María; Reyes, Jessica Karina; Restrepo, Leda; Domínguez-Borbor, Cristóbal; Maldonado, Martha; Bayot, Bonny

doi:10.1371/journal.pone.0210478

Cited by 18 publications

(16 citation statements)

References 60 publications

(66 reference statements)

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“…They can also reduce the ability of bacterial pathogens to adhere to epithelial cells in vitro (e.g., Daphnetin, a coumarin-derivative) by reducing the expression of H. pylori genes involved in colonisation [30]. Antimicrobial mixtures of probiotics, organic acids and essential oils were effective in reducing the devastating impact of Vibrio strain infections in South American farmed shrimps [31]; however, the study did not elucidate the biological mechanism responsible for the effect.…”

Section: Discussionmentioning

confidence: 94%

Attenuation of Vibrio parahaemolyticus Virulence Factors by a Mixture of Natural Antimicrobials

et al. 2019

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Reducing acute mortality in aquatic crustaceans using natural alternatives to antibiotics has become a necessity, firstly for its positive impact on the aquaculture industry and, secondly, because the extensive use of antibiotics may lead to increased levels of drug resistance in pathogenic microorganisms. This study aimed to investigate the effect of a mixture of natural antimicrobials on the in vitro and in vivo virulence abilities of Type VI secretion system (T6SS)-positive Vibrio parahaemolyticus (A3 and D4), strains known as having potentially harmful health consequences for aquatic crustaceans and consumers. Herein, we report that a natural antimicrobial mixture (A3009) was capable of significantly reducing the virulence of V. parahaemolyticus strains A3 and D4 in an in vitro infection model, using the fish cell line CHSE-214, an effect which correlates with the bacterial downregulation of hcp1 and hcp2 gene expression and with the ability of the antimicrobial to efficiently retain low cytotoxic levels (p < 0.001). We show for the first time that a natural antimicrobial is able to significantly reduce the mortality of shrimps in a challenge experiment and is able to significantly attenuate H 2 O 2 release during infection (p < 0.001), indicating that it could harbor positive intestinal redox balance effects.

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Section: Discussionmentioning

confidence: 94%

Attenuation of Vibrio parahaemolyticus Virulence Factors by a Mixture of Natural Antimicrobials

et al. 2019

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“…In addition, well-documented toxic effects of florfenicol on fish and crustacean larvae [69][70][71][72] indicate the urgent need for considering the use of alternative strategies to control pathogenic bacteria in live feeds administered to marine fish aquaculture, including bacteriophages, probiotics, and natural products [42,73,74]. In a recent study, probiotics, organic acids, and essential oils were demonstrated to be effective at controlling the pathogenic activity of vibrios on A. franciscana nauplii [75]. Thus, using live feeds as carriers for non-antibiotic therapeutic applications in marine fish cultures appears to be a very attractive option.…”

Section: Discussionmentioning

confidence: 99%

Live Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)

Hurtado

Miranda

Rojas

et al. 2020

Animals

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Simple Summary: The culture of the marine fish red cusk eel Genypterus chilensis is currently considered a priority for Chilean aquaculture but low larval survival rates have prompted the need for the continuous use of antibiotics, mainly florfenicol. In this study, the role of live prey (rotifers and the brine shrimp Artemia franciscana) used to feed fish larvae as a source of antibacterial-resistant bacteria in a commercial culture of G. chilensis was investigated. Samples of live feeds were collected during the larval growth period and their bacterial contents were determined. High levels of potentially opportunistic pathogens, such as Vibrio spp., as well as florfenicol-resistant bacteria, were detected. Sixty-five florfenicol-resistant isolates were recovered from these cultures and identified as Vibrio (81.5%) and Pseudoalteromonas (15.4%), which exhibited a high incidence of co-resistance to the antibiotics streptomycin, oxytetracycline, co-trimoxazole, and kanamycin. The majority of them carried the florfenicol-resistance encoding genes floR and fexA. The high prevalence of antibiotic-resistant bacteria and the associated genetic elements in live feed administered to reared fish larvae requires the prompt implementation of efficient management strategies to prevent future therapy failures in fish larval cultures and the spread of antibiotic-resistant bacteria to associated aquatic environments. Abstract:The culture of red cusk eel Genypterus chilensis is currently considered a priority for Chilean aquaculture but low larval survival rates have prompted the need for the continuous use of antibacterials. The main aim of this study was to evaluate the role of live feed as a source of antibacterial-resistant bacteria in a commercial culture of G. chilensis. Samples of rotifer and Artemia cultures used as live feed were collected during the larval growth period and culturable bacterial counts were performed using a spread plate method. Rotifer and Artemia cultures exhibited high levels of resistant bacteria (8.03 × 10 4 to 1.79 × 10 7 CFU/g and 1.47 × 10 6 to 3.50 × 10 8 CFU/g, respectively). Sixty-five florfenicol-resistant isolates were identified as Vibrio (81.5%) and Pseudoalteromonas (15.4%) using 16S rRNA gene sequence analysis. A high incidence of resistance to streptomycin (93.8%), oxytetracycline (89.2%), co-trimoxazole (84.6%), and kanamycin (73.8%) was exhibited by resistant isolates. A high proportion of isolates (76.9%) carried the florfenicol-resistance encoding genes floR and fexA, as well as plasmid DNA (75.0%).Animals 2020, 10, 505 2 of 23 microbiota in reared fish larvae, thus proper monitoring and management strategies for live feed cultures appear to be a priority for preventing future therapy failures in fish larval cultures.

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“…Antibiotics and probiotics are some of the common treatment methods for most strains of pathogenic bacteria found in aquaculture systems (Yu et al 2012;Miranda et al 2013;Sotomayor et al 2019). However, the use of antibiotics often resulted in the development of multidrugresistant strains, raising concerns about consumer's health and economic threat (Miranda et al 2013;Lee et al 2018) whereas probiotics are species-specific, involve precise dosage administration, and research on probiotic as biocontrol is still limited (Hosseinifar et al, 2018).…”

Section: Bacteriamentioning

confidence: 99%

“…2013; Sotomayor et al . 2019). However, the use of antibiotics often resulted in the development of multidrug‐resistant strains, raising concerns about consumer’s health and economic threat (Miranda et al .…”

Section: Disease In Aquaculturementioning

confidence: 99%

Protein–protein interaction network: an emerging tool for understanding fish disease in aquaculture

Waiho

Afiqah‐Aleng

Iryani

et al. 2020

Reviews in Aquaculture

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Protein-protein interactions (PPIs) play integral roles in a wide range of biological processes that regulate the overall growth, development, physiology and disease in living organisms. With the advancement of high-throughput sequencing technologies, increasing numbers of PPI networks are being predicted and annotated, and these contribute greatly towards the understanding of pathogenesis and the discovery of novel drug targets for the treatment of diseases. The use of this tool is gaining popularity in the identification, understanding and treatment of diseases in humans and plants. Due to the importance of aquaculture in tackling the global food crisis by producing cheap and high-quality protein source, the maintenance of the overall health status of aquaculture species is essential. With the increasing omics data on aquaculture species, the PPI network is an emerging tool for fish health maintenance. In this review, we first introduce the concept of PPI network, how they are discovered and their general application. Then, the current status of aquaculture and disease in aquaculture are discussed. The different applications of PPI network in aquaculture fish disease management such as biomarker identification, mechanism prediction, understanding of host-pathogen interaction, understanding of pathogen co-infection interaction, and potential development of vaccines and treatments are subsequently highlighted. It is hoped that this emerging tool-PPI networkwould deepen our understanding of the pathogenesis of various diseases and hasten the prevention and treatment processes in aquaculture species.

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Efficacy assessment of commercially available natural products and antibiotics, commonly used for mitigation of pathogenic Vibrio outbreaks in Ecuadorian Penaeus (Litopenaeus) vannamei hatcheries

Cited by 18 publications

References 60 publications

Attenuation of Vibrio parahaemolyticus Virulence Factors by a Mixture of Natural Antimicrobials

Attenuation of Vibrio parahaemolyticus Virulence Factors by a Mixture of Natural Antimicrobials

Live Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)

Protein–protein interaction network: an emerging tool for understanding fish disease in aquaculture

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