Efficacy assessment of an inactivated Tembusu virus vaccine candidate in ducks

Zhang, Lijiao; Li, Zhanhong; Zhang, Qingshui; Sun, Mengxu; Li, Shuang; Su, Wenliang; Hu, Xueying; He, Wenqi; Su, Jingliang

doi:10.1016/j.rvsc.2016.11.002

Cited by 26 publications

(16 citation statements)

References 37 publications

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“…In affected flocks, the egg production rate may reduce to 10% or less within ∼1 week after disease onset (23). To control the disease, several vaccine candidates have been developed, including live-attenuated (24), inactivated (25,26), and subunit-based (27-31) vaccine candidates. Live-attenuated and inactivated vaccines have been licensed to use in ducks in China (32,33).…”

Section: Introductionmentioning

confidence: 99%

Detection of Neutralizing Antibodies to Tembusu Virus: Implications for Infection and Immunity

Yang

et al. 2019

Front. Vet. Sci.

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Section: Introductionmentioning

confidence: 99%

Detection of Neutralizing Antibodies to Tembusu Virus: Implications for Infection and Immunity

Yang

et al. 2019

Front. Vet. Sci.

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“…To date, DTMUV-related studies have focused on pathogenicity [21,22], detection methods [23,24], vaccines [10,25,26], transmission routes [27], genetic evolution and epidemiology [1,28,29], and molecular structure [30,31]. Unfortunately, the immune mechanisms of DTMUV invasion and infection in host cells remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis of Immune-Related Gene Expression in Duck Embryo Fibroblasts Following Duck Tembusu Virus Infection

Lin

Tang

et al. 2018

IJMS

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Duck is a major waterfowl species in China, providing high-economic benefit with a population of up to 20–30 billion per year. Ducks are commonly affected by severe diseases, including egg-drop syndrome caused by duck Tembusu virus (DTMUV). The immune mechanisms against DTMUV invasion and infection remain poorly understood. In this study, duck embryo fibroblasts (DEFs) were infected with DTMUV and harvested at 12 and 24 h post-infection (hpi), and their genomes were sequenced. In total, 911 (764 upregulated and 147 downregulated genes) and 3008 (1791 upregulated and 1217 downregulated) differentially expressed genes (DEGs) were identified at 12 and 24 hpi, respectively. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that DEGs were considerably enriched in immune-relevant pathways, including Toll-like receptor signaling pathway, Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Chemokine signaling pathway, NOD-like receptor signaling pathway, and Hematopoietic cell lineage at both time points. The key DEGs in immune system included those of the cytokines (IFN α2, IL-6, IL-8L, IL-12B, CCR7, CCL19, and CCL20), transcription factors or signaling molecules (IRF7, NF-κB, STAT1, TMEM173, and TNFAIP3), pattern recognition receptors (RIG-I and MDA5), and antigen-presenting proteins (CD44 and CD70). This suggests DTMUV infection induces strong proinflammatory/antiviral effects with enormous production of cytokines. However, these cytokines could not protect DEFs against viral attack. Our data revealed valuable transcriptional information regarding DTMUV-infected DEFs, thereby broadening our understanding of the immune response against DTMUV infection; this information might contribute in developing strategies for controlling the prevalence of DTMUV infection.

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“…Vaccines remain the most cost-effective strategy to control these pathogens. However, the available oilemulsified, inactivated vaccines usually take six weeks to confer immune protection (Lin et al, 2015;Zhang et al, 2017), and two or three doses are required for adequate protection (Halstead and Thomas, 2011). Moreover, they can cause severe adverse reactions, including local inflammation and "egg drop" (Zou et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Recombinant Newcastle disease virus (NDV) expressing Duck Tembusu virus (DTMUV) pre-membrane and envelope proteins protects ducks against DTMUV and NDV challenge

Sun

Dong

et al. 2018

Veterinary Microbiology

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The newly emerged Duck Tembusu virus (DTMUV) is responsible for considerable economic loss in waterfowl-raising areas in China since 2010. Meanwhile, the virulent Newcastle disease virus (NDV) has also caused sporadic outbreaks in waterfowl. The individual vaccines against both diseases are available, however, there is no bivalent or combined vaccine for either disease. Here, we constructed a recombinant NDV-vectored vaccine candidate that expresses the pre-membrane (prM) and envelope (E) genes from DTMUV, designated as aGM/prM + E. The foreign prM and E proteins were stably expressed in aGM/prM + E and exhibited similar pathogenicity but higher growth kinetics than those of the parental virus. The aGM/prM + E carries a fusion cleavage site in accordance with avirulent viruses that have been frequently isolated from waterfowl, and induced remarkably (p < 0.001) higher NDV-specific hemagglutination inhibition (HI) titers than commercially available live NDV vaccines (LaSota strain). The aGM/prM + E also elicited significantly higher (p < 0.05) virus neutralization (VN) titers than commercially available DTMUV inactivated vaccines (HB strain). The aGM/prM + E not only provided complete protection against NDV challenge but also reduced the gross lesions on ovarian folliculi and provided 80% protection against DTMUV in ducks. We note that the aGM/prM + E vaccine can prevent challenged ducks from shedding of NDV and DTMUV. Our results suggest that the candidate vaccine aGM/prM + E would help decrease NDV and DTMUV transmissions in waterfowl raising areas in China.

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Efficacy assessment of an inactivated Tembusu virus vaccine candidate in ducks

Cited by 26 publications

References 37 publications

Detection of Neutralizing Antibodies to Tembusu Virus: Implications for Infection and Immunity

Detection of Neutralizing Antibodies to Tembusu Virus: Implications for Infection and Immunity

Comparative Transcriptomic Analysis of Immune-Related Gene Expression in Duck Embryo Fibroblasts Following Duck Tembusu Virus Infection

Recombinant Newcastle disease virus (NDV) expressing Duck Tembusu virus (DTMUV) pre-membrane and envelope proteins protects ducks against DTMUV and NDV challenge

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