Comparative Transcriptomic Analysis of Immune-Related Gene Expression in Duck Embryo Fibroblasts Following Duck Tembusu Virus Infection

Yu, Guanliu; Lin, Yun; Tang, Yi; Diao, Youxiang

doi:10.3390/ijms19082328

Cited by 22 publications

(36 citation statements)

References 54 publications

(68 reference statements)

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“…However, other quantitative proteomic analyses revealed that DDX5 was upregulated (1.84 fold) in DEF cells infected with DTMUV [102]; the result was different from DDX5 detection in mammalian cells, indicating that there might be a difference in the innate immune responses to DTMUV between avians and mammals. Additionally, DDX42, DDX60, DHX15, and mov10 RISC complex RNA helicase were differentially expressed after DTMUV infection [82,102], suggesting that these helicase proteins should be involved in DTMUV infection, but the specific roles were unclear.…”

Section: Other Prrs Relevant To Dtmuv Infectionmentioning

confidence: 99%

Innate immune responses to duck Tembusu virus infection

Zhao

Yang

et al. 2020

Vet Res

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The disease caused by duck Tembusu virus (DTMUV) is characterized by severe egg-drop in laying ducks. Currently, the disease has spread to most duck-raising areas in China, leading to great economic losses in the duck industry. In the recent years, DTMUV has raised some concerns, because of its expanding host range and increasing pathogenicity, as well as the potential threat to public health. Innate immunity is crucial for defending against invading pathogens in the early stages of infection. Recently, studies on the interaction between DTMUV and host innate immune response have made great progress. In the review, we provide an overview of DTMUV and summarize current advances in our understanding of the interaction between DTMUV and innate immunity, including the host innate immune responses to DTMUV infection through pattern recognition receptors (PRRs), signaling transducer molecules, interferon-stimulated genes (ISGs), and the immune evasion strategies employed by DTMUV. The aim of the review is to gain an in-depth understanding of DTMUV pathogenesis to facilitate future studies.

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Section: Other Prrs Relevant To Dtmuv Infectionmentioning

confidence: 99%

Innate immune responses to duck Tembusu virus infection

Zhao

Yang

et al. 2020

Vet Res

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“…Duck, as a major fowl species in China, offers high-economic benefits with a population of up to 20–30 billion per year [ 1 ]. Unfortunately, hemorrhagic ovarian inflammation, which is also named egg-drop syndrome and is mainly caused by Tembusu virus (TMUV), has resulted in large economic losses of several billion dollars for the Chinese duck-rearing industry since April 2010 [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China

Lin

Tang

et al. 2018

Viruses

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Tembusu virus (TMUV) is a contagious pathogen from fowl that mainly infects ducks and geese, causing symptoms of high fever, loss of appetite, retarded growth, neurological symptoms, severe duck-drop syndrome, and even death. During an epidemiological investigation of TMUV in Northern China, we isolated 11 TMUV strains from ducks, chickens, geese, sparrows, and mosquitoes (2011–2017). Phylogenetic analysis of the open-reading frames of genes revealed that these strains clustered into Chinese strains II. The nucleotide and amino acid homologies of NS1 of the strains ranged between 85.8–99.8% and 92.5–99.68%, respectively, which were lower than those of E (86.7–99.9% and 96.5–99.9%, respectively), NS3 (87.6–99.9% and 98.2–99.8%, respectively), and NS5 (86.5–99.9% and 97.8–99.9%, respectively). Predictions of the tertiary structure of the viral proteins indicated that NS1 in 4 of 11 strains had a protein structure mutation at 180TAV182 that changed a random crimp into an alpha helix. The protein of 6 of 11 strains had a glycosylation site mutation from NTTD to NITD. Furthermore, epidemiological data suggested that TMUV has been circulating in half of China’s provinces (17 of 34). Our findings, for the first time, have identified the NS1 protein as a potential hypervariable region for genetic evolution. Additionally, the territorial scope of the virus has expanded, requiring strict bio-security measures or a multivalent vaccine to control its spread.

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“…Although Yu et al [22] reported expression changes for some innate immune cytokines in DTMUV-infected DEF cells, the degrees of change and trends of expression were not described. To address this, we infected DEF cells with DTMUV and assessed the expression of TLRs, RLRs, inflammatory cytokines and type I IFN at different time points.…”

Section: Tlr3 and Mda5 Play Major Immunity Roles In Dtmuv-infected Dementioning

confidence: 99%

“…Based on a previous study [21], DTMUV infection of CEF cells and HEK293 cells can trigger a robust innate immune response, in which TLR3 and MDA5 play an important role. Moreover, Yu et al [22] performed high-throughput sequencing and found that DTMUV-infected DEF cells exhibit various innate immunity processes, which notably include the TLRs and RLRs signaling pathways. Moreover, we found other flaviviruses, like dengue virus (DENV), Zika virus (ZIKV) and West Nile virus (WNV) can also trigger robust innate immunity processes including TLRs and RLRs signaling pathways.…”

Section: Dtmuv-infected Ducks Show Up-regulated the Expression Of Tlrmentioning

confidence: 99%

“…MAVS, in turn, recruits TRAF3 to phosphorylate IRF3 and IRF7 and initiate the type I IFN antiviral immune response [20]. Chen et al described the innate immune process that DTMUV induces in chicken [21], while Yu et al detailed changes in the expression of inflammatory factors upon infection of duck with DTMUV [22]. Moreover, He Y et al reveals the innate immune response to DTMUV infection in geese [5].…”

Section: Introductionmentioning

confidence: 99%

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DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression

Guo

Cheng

Zhang

et al. 2020

Viruses

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Duck tembusu virus (DTMUV) is a single-stranded, positive-polarity RNA flavivirus that has caused considerable economic losses in China in recent years. Innate immunity represents the first line of defense against invading pathogens and serves as an important role in resisting viral infections. In this study, we found that the infection of ducks by DTMUV triggers Toll-like receptors (TLRs) and (RIG-I)-like receptors (RLRs) signaling pathways and inducing abundant of pro-inflammatory factors and type I interferons (IFNs), in which melanoma differentiation-associated gene 5 (MDA5) and Toll-like receptor 3 (TLR3) play important immunity roles, they can inhibit the replication process of DTMUV via inducing type I IFNs. Moreover, we demonstrated that type I IFNs can inhibit the DTMUV replication process in a time- and dose-dependent manner. Exosomes are small membrane vesicles that have important roles in intercellular communication. MicroRNAs (miRNAs) are small non-coding RNAs that can modulate gene expression and are common substances in exosomes. In our experiment, we successfully isolated DEF cells derived exosome for the first time and explored its function. Firstly, we found the expression of miR-148a-5p is significantly decreased following DTMUV infect. Then we found miR-148a-5p can target TLR3 and down-regulate the expression of TLR3, serving as a negative factor in innate immunity. Unfortunately, we cannot find miRNAs with different expression changes that can target MDA5. Lastly, our experimental results showed that TLR3 was one of the causes of miR-148a-5p reduction, suggesting that the high level of TLR3 after DTMUV infect can both trigger innate immunity and suppress miR-148a-5p to resist DTMUV.

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Comparative Transcriptomic Analysis of Immune-Related Gene Expression in Duck Embryo Fibroblasts Following Duck Tembusu Virus Infection

Cited by 22 publications

References 54 publications

Innate immune responses to duck Tembusu virus infection

Innate immune responses to duck Tembusu virus infection

Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China

DEF Cell-Derived Exosomal miR-148a-5p Promotes DTMUV Replication by Negative Regulating TLR3 Expression

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