Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders

Othman, Fadwa; El-Badry, Diya A.; Mohamed, M. Y.; Shaker, Mona H.; ATorky, Hossam

doi:10.5455/ajvs.202266

Cited by 4 publications

(11 citation statements)

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“…Similar finding has reported that administration of griseofulvin evokes necrosis in the seminal vesicle and minimized the output of the sperm in rats [21]. In another study, it was shown that griseofulvin induced oligospermia in sheep [22] and elicited adverse effects on sperms of mice [23]. In the same line, griseofulvin has been found to affect the germ cells of male mice and to reduce the serum level of testosterone [28].…”

Section: Discussionsupporting

confidence: 70%

“…On the other hand, fluconazole has been shown to decrease sperm cells motility and progressivity [34]. High doses of griseofulvin have been shown to cause a deterioration of frozen semen quality [22]. It has been reported that ketoconazole had a spermicidal action if mixed with ejaculated sperm (in vitro) obtained from dogs, monkeys and humans [30].…”

Section: Discussionmentioning

confidence: 99%

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The effects of oral ketoconazole and griseofulvin on the fertility of male rabbits

Mohamed¹,

Hassan²,

Amer

et al. 2020

Mansoura Veterinary Medical Journal

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T o inves tig ate poss ible s ide e ffe cts of ke tocona z ole a nd g ris e ofulvin on fertility of clinica lly healthy male rabbits . Design: Random iz e d controlle d experim e ntal study. Animals : Thirty mature male rabbits . Proce dure s: Ra bbits we re ra ndom ly a lloca te d into thre e e qua l siz e d g roups (10 a nimals ea ch). T he firs t g roup s e rve d as a control g roup (re ceive d no tre atm e nt), the s e cond group re ce ive d g ris e ofulvin (25 mg / kg B.W ) orally for 30 da ys , a nd the third g roup re ce ive d ke tocona z ole (50 mg / kg B.W ) ora lly for 30 da ys . Se m e n sa m ple s we re colle cte d afte r 1 da y, 1 we e k a nd 2 we e ks post -a dm inistra tion to de te rm ine s pe rm m otility, % of live a nd de a d s pe rm s , tota l s pe rm a bnorm a lities a nd s pe rm cell conce ntra tion. T wo we e ks pos t-a dm inis tration, tes tes a nd e pididym ides we re re m ove d for histopa tholog ica l examination. Re s ults: Both drug s produce d a sig nifica nt re duction in the se rum tes tos te rone le ve l, sperm cell concentra tion, percent of live sperms and percent of sperm motility. Conclus ion and clinical re le vance: Ke toconaz ole a nd g ris e ofulvin ha ve a ne ga tive impa ct on fertility of male rabbits , and the effect is more pronounce d with ketoconaz ole .

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

The effects of oral ketoconazole and griseofulvin on the fertility of male rabbits

Mohamed¹,

Hassan²,

Amer

et al. 2020

Mansoura Veterinary Medical Journal

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“…For instance, amphotricin-B and griseofulvin resulted in drastic deterioration of frozen spermatozoa quality in a dose-dependent manner, as summarized by Othman et al (2016). However, addition of fluconazole to sheep semen diluent has a benefit of improving the quality of yeast contaminated sperm with no negative effect on the frozen-thawed semen quality as Othman et al (2016) have recently demonstrated. Therefore, the suitability of, at least, some antimycotics to decrease yeasts contamination in processed ram semen might be concluded.…”

Section: Approaches For Yeasts Eliminationmentioning

confidence: 99%

“…In livestock, yeasts have been implicated as causes of bovine reproductive problems, including abortion in cows and infertility in bulls, and equine uterine infections, as reviewed by Othman et al (2016).…”

Section: Semen Contamination With Yeastsmentioning

confidence: 99%

Pathogens in Processed Ram Semen and Approaches for Their Elimination

Savvulidi

Ptáček

Stádník

2018

Acta Univ. Agric. Silvic. Mendelianae Brun.

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A variety of pathogenic contaminants might be isolated from semen, processed for artificial insemination (AI) programs: prions, viruses, bacteria, yeasts or protozoa. This review will discuss the broad scale of pathogens detected in processed ram semen. Reviewed findings will be confronted with the latest knowledge related to semen contamination in other livestock species. An accent will be placed on current experience and future aspects of approaches for elimination of pathogens from processed semen (especially, from semen processed under field conditions).

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“…To minimize these adverse effects, antimicrobials are included in the composition of some semen extenders to reduce the pollution or eliminate bacterial and fungal contamination during the semen storage and processing (Corona & Cherchi, 2009; Olivieri et al., 2011; Yániz et al., 2010). Unfortunately, fungicides can be harmful to the overall fertility of the sperm (Othman et al., 2016), and there is evidence that antifungal agents tend to be highly spermicidal to bull and rabbit sperm (Foote, 2002), so their use in semen extenders is not very common. The use of conventional antifungals such as amphotericin‐B and griseofulvin have shown a drastic deterioration of sheep‐frozen semen quality in a dose‐dependent manner (Othman et al., 2016), while other agents, such as fluconazole, have shown positive results for the quality of sheep (Othman et al., 2016) and porcine semen (Ciornei et al., 2009).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effect of conventional and natural antifungals on motility and kinetics of cooled stallion semen

Suarez

Gutiérrez

López

et al. 2022

Reprod Domestic Animals

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Microbial growth in semen may cause a decline of sperm quality and fertility; however, the addition of antifungals to semen extender has been shown to impair the overall fertility of the sperm. The aim of this study was to evaluate the antifungal activity of conventional and natural compounds, and their effect on the motility and kinetics of cooled stallion semen. A total of 15 ejaculates from five stallions were collected using the artificial vagina. Each ejaculate was supplemented with: fluconazole at 12.5 (F1), 25 (F2) and 50 (F3) mg/ml; amphotericin‐B at 6.5 (A1), 12.5 (A2) and 25 (A3) mg/ml (A3); clotrimazole at 12.5 (C1), 25 (C2) and 50 (C3) mg/ml; isoespintanol at 50 (I1), 100 (I2) and 150 (I3) µM; thymol at 50 (T1), 100 (T2) and 150 (T3) µM; and a control without supplementation. Motility and kinetics of semen at 0, 24 and 48 hr of cooling at 15°C were assessed using computer‐assisted sperm analysis (CASA). At hour 48 of cooling, the antifungal effect of the treatments was evaluated. At hour 0 of cooling, amphotericin‐B and I3 showed a reduction in most of the motility and kinetic parameters evaluated (p < .05). These treatments, and also C2 and C3, showed similar results at 24 and 48 hr of cooling. Thymol maintained motility and kinetics of the spermatozoa at all evaluated refrigeration times. Besides, I2 showed a decrease (p < .05) in the colony‐forming unit compared to that in the control. It is concluded that thymol and isospintanol could be added as natural antifungals in extenders for stallion semen refrigeration.

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Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders

Cited by 4 publications

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The effects of oral ketoconazole and griseofulvin on the fertility of male rabbits

The effects of oral ketoconazole and griseofulvin on the fertility of male rabbits

Pathogens in Processed Ram Semen and Approaches for Their Elimination

Evaluation of the effect of conventional and natural antifungals on motility and kinetics of cooled stallion semen

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