Effet d'un traitement par l'acide abscisique sur la division cellulaire, les teneurs en ADN et l'élongation du bourgeon cotylédonaire de plants de pois décapités

Nougarède, Arlette; Rondet, Pierre; Landré, Pierre; Rembur, Jacques

doi:10.1139/b87-125

Cited by 14 publications

(12 citation statements)

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“…Previous studies have shown that exogenously applied ABA can inhibit mitotic activity in meristems of several plant systems (14,25,34). In recent studies with in vitro cultured maize kernels (23) ABA applied to the culture medium increased ABA concentrations in endosperm to 100 ng/g fresh weight, similar to concentrations observed in apical kernels of water-limited plants in the present study (Fig.…”

Section: Aba Concentrationssupporting

confidence: 88%

“…In developing maize kernels, ABA concentrations increased when water deficit occurred during endosperm cell division (26). Abscisic acid is associated with water deficit in many tissue systems and is generally regarded as a growth inhibitor, yet only a few reports have documented its effect on cell division (2,14,25,34). In recent studies of in vitro cultured maize kernels, ABA supplied to culture media inhibited endosperm cell division and kernel growth (23).…”

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confidence: 99%

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Influence of Water Deficit on Maize Endosperm Development

Ober¹,

Setter²,

Madison³

et al. 1991

Plant Physiol.

132

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In maize (Zea mays L.), drought during the post-pollination stage decreases kernel growth and often leads to grain yield losses. Kernels in the apical region of the ear are more severely affected than basally positioned kernels. We hypothesized that water deficit during early endosperm development might inhibit kernel growth by decreasing endosperm cell division, and that this response might be mediated by changes in endosperm abscisic acid (ABA) levels. Greenhouse-grown maize, cultivar Pioneer 3925, was subjected to water limitation from 1 to 15 days after pollination (DAP), spanning the period of endosperm cell division and induction of storage product accumulation. Water deficit decreased the number of endosperm nuclei during the treatment period; the most substantial effect was in the apical region of ears. Correspondingly, endosperm fresh weight, starch accumulation and dry mass at maturity were decreased by water limitation. Abscisic acid concentrations in endosperm were quantified by enzyme-linked immunosorbent assay. Water deficit increased ABA concentration in apical-region endosperm by fourfold compared to controls. ABA concentrations were also increased in middle and basal regions of the ear, but to a lesser extent. Two key enzymes in the starch synthesis pathway, sucrose synthase and granule-bound ADP-glucose starch synthase, and zein, the major storage protein in maize endosperm, were studied as markers of storage product synthesis. Water deficit did not affect sucrose synthase enzyme activity or RNA transcript abundance relative to total RNA. However, ADP-glucose starch synthase activity and RNA transcript abundance decreased slightly in apical-region endosperm of water-limited plants by 15 DAP, compared with well-watered controls. In contrast to starch, there was no treatment effect on the accumulation of zein, evaluated at either the polypeptide or RNA level. We conclude that under the conditions tested, the establishment of starch and zein synthetic potential in endosperm was only slightly affected by plant water deficit during the early phase of kernel growth, and that capacity for growth and starch accumulation was affected by the extent to which cell division was inhibited. Based on correlative changes in ABA concentration and cell division we suggest that ABA may play a role in inhibiting endosperm cell division during water limitation.

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Section: Aba Concentrationssupporting

confidence: 88%

mentioning

confidence: 99%

Influence of Water Deficit on Maize Endosperm Development

Ober¹,

Setter²,

Madison³

et al. 1991

Plant Physiol.

132

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“…Barlow and Pilet (3) reported a diminished rate of cell division in maize root tips following exogenously applied 50 ,uM, but not 5 AM ABA. Similarly, Nougarede et al (20) demonstrated that 10 to 1000 ,M ABA inhibited cell division in decapitated, meristematic pea buds. Both investigators concluded that ABA reduced the rate of cell division, but even at high concentrations (100-1000 ,uM), ABA did not completely inhibit cell division.…”

Section: Discussionmentioning

confidence: 84%

“…The rate of cell division in maize root tips was diminished with exogenously applied ABA (3). Cell division was also inhibited in decapitated pea buds after applications of 10 to 100 Mm ABA (20). In wheat pollen, exposure to ABA or water stress caused sterility by decreasing the rate of meiosis in pollen mother cells (25).…”

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confidence: 98%

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Abscisic Acid Inhibition of Endosperm Cell Division in Cultured Maize Kernels

Myers¹,

Setter²,

Madison³

et al. 1990

Plant Physiol.

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The response of developing maize (Zea mays L.) endosperm to elevated levels of abscisic acid (ABA) was investigated. Maize kernels and subtending cob sections were excised at 5 days after pollination (DAP) and placed in culture with or without 90 micromolar (±)-ABA in the medium. A decreased number of cells per endosperm was observed at 10 DAP (and later sampling times) in kernels cultured in medium containing ABA from 5 DAP, and in kernels transferred at 8 DAP to medium containing ABA, but not in kernels transferred at 11 DAP to medium containing ABA. The number of starch granules per endosperm was decreased in some treatments, but the reduction, when apparent, was comparable to the decreased number of endosperm cells. The effect on endosperm fresh weight was slight, transient, and appeared to be secondary to the effect on cell number. Mature endosperm dry weight was reduced when kemels were cultured continuously in medium containing ABA. Endosperm (+)-ABA content of kernels cultured in 0, 3, 10, 30, 100, or 300 micromolar (±)-ABA was measured at 10 DAP by indirect ELISA using a monoclonal antibody. Content of (+)-ABA in endosperms correlated negatively (R = -0.92) with endosperm cell number. On the basis of these studies we propose that during early kernel development, elevated levels of ABA decrease the rate of cell division in maize endosperm which, in turn, could limit the storage capacity of the kernel.

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Fate of the nucleolar vacuole during resumption of cell cycle in pea cotyledonary buds

et al. 2000

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Summary. Meristematic cells of pea cotyledonary buds blocked inG~I state contain a small nucleolus with a large central clear area surrounded by a fibrillar rim. The nucleolar structure varies according to the cell cycle from the G~l-blocked state until the first mitoses occurring between 24 and 27 h after removal of the main stem. In order to better identify and understand the role of the central area in the nucleolar function, its content was investigated by cytochemical and terminal deoxynucleotidyl transferase-immunogold methods. The central area showed the characteristics of a vacuole commonly constituted of the condensed chromatin, ribonucleoprotein granules, and lack of argyrophilic proteins. 3h after decapitation, a thickening of the fibrillar rim occurred, accompanied by an increase of granules in the vacuole. After 6h, the unique vacuole broke up into two to four small vacuoles in which the granules are more abundant. After 12 h the nucleolus acquired compact structure with few minute vacuoles dispersed over the fibrillar component. During the whole cell cycle, the condensed chromatin is always observed in the vacuole. Our findings suggest that the appearance of the vacuoles is subsequent to the output of preribosomes from nucleolus. These vacuoles might play a role in condensation and decondensation of the chromatin.

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Effet d'un traitement par l'acide abscisique sur la division cellulaire, les teneurs en ADN et l'élongation du bourgeon cotylédonaire de plants de pois décapités

Cited by 14 publications

References 0 publications

Influence of Water Deficit on Maize Endosperm Development

Influence of Water Deficit on Maize Endosperm Development

Abscisic Acid Inhibition of Endosperm Cell Division in Cultured Maize Kernels

Fate of the nucleolar vacuole during resumption of cell cycle in pea cotyledonary buds

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