Abscisic Acid Inhibition of Endosperm Cell Division in Cultured Maize Kernels

Myers, Patricia N.; Setter, Tim L.; Madison, James T.; Thompson, John F.

doi:10.1104/pp.94.3.1330

Cited by 66 publications

(43 citation statements)

References 26 publications

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“…There are several, possibly interacting, factors contributing to the variation in grain weight. It may result from intra-panicle and intra-spikelet differences in development rate and time of pollination that are reflected in duration of grain-filling period (Bonnett 1961a, Tibelius andKlinck 1987), vascular transport capacity (Hanif and Langer 1972, shown in wheat;Housley and Peterson 1982), cell division rate and duration of developing grains as shown for wheat (Brocklehurst 1977, Radley 1978, and/or hormonal balance as shown for barley and maize (Michael and Seiler-Kelbitch 1972, Mounler et al 1980, Myers et al 1990, Ober et al 1991.…”

Section: Rajala a And Peltonen-sainio P Intra-plant Variation In Dementioning

confidence: 99%

Intra-plant variation for progress of cell division in developing oat grains: a preliminary study

Rajala¹,

Peltonen‐Sainio²

2008

AFSci

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Development of an oat panicle proceeds from the uppermost terminal spikelet downward to the base of the panicle. The intra-panicle variation in development is likely to influence potential grain size. This may result from differences in activity and duration of the cell division phase, vascular transport capacity, duration of filling period and/or hormonal balance. In this preliminary survey we studied intra-panicle variation in pollination (when clusters of pollen were visibly attached to stigmatic branches) of florets and cell division in developing grains immediately after pollination of the oat cultivars Belinda and Fiia. We found substantial intra-panicle variation for both traits. The highest cell number was found in the uppermost, most advanced, primary grain, while secondary grains tended to have fewer cells compared with their counterpart primary grains irrespective of their position. Results of an additional experiment indicated that death of the primary floret in the conventional oat cultivar Virma prior to pollination, resulted in higher weight of secondary grain, though this never equalled that of the primary counterpart. This limited growth capacity may partly result from lower cell number. These results encourage us to continue with experiments on the contribution of groat cell number to sink strength and grain-filling capacity.

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Section: Rajala a And Peltonen-sainio P Intra-plant Variation In Dementioning

confidence: 99%

Intra-plant variation for progress of cell division in developing oat grains: a preliminary study

Rajala¹,

Peltonen‐Sainio²

2008

AFSci

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“…Endosperms were dissected out and treated with pectinase to obtain a homogeneous suspension of nuclei as previously described (13). Nuclei were stained with acetocarmine and counted on a hemacytometer (X400; Improved Neubauer Ultra Plane, Model 3500, Hausser Scientific, Blue Bell, PA).…”

Section: Cell Number Determinationmentioning

confidence: 99%

“…Explants of whole kernels and subtending cob sections were cultured at 5 DAP as previously described (13). Combinations in an array of three sucrose concentrations (0.29, 0.44, or 0.58 M) and three sorbitol concentrations (0, 0.15 or 0.29 M) were used to obtain six media at three levels of Ow (Table I).…”

Section: In Vitro Culturementioning

confidence: 99%

“…Our previous work indicated that ABA inhibits cell division in developing maize endosperm (13). Additionally, Ober et al (16) reported that plant water deficit caused a decrease in endosperm cell number concomitant with a rise in ABA.…”

mentioning

confidence: 99%

“…Additionally, Ober et al (16) reported that plant water deficit caused a decrease in endosperm cell number concomitant with a rise in ABA. The ABA detected in the endosperm was imported from matemal tissues (15 (13). The ear shoots were bagged prior to silk emergence and all florets on each ear were synchronously pollinated 4 or 5 d after silk emergence.…”

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confidence: 99%

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Endosperm Cell Division in Maize Kernels Cultured at Three Levels of Water Potential

Myers¹,

Setter²,

Madison³

et al. 1992

Plant Physiol.

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The influence of osmoticum treatments on early kernel development of maize (Zea mays L.) was studied using an in vitro culture method. Kernels with subtending cob sections were placed in culture at 5 days after pollination. Sucrose (0.29, 0.44, or 0.58 molar) and sorbitol (0, 0.15, or 0.29 molar) were used to obtain six media with water potentials of -1.1, -1.6, or -2.0 megapascals. Kernel water potential declined in correspondence with the water potential of the medium; however, fresh weight growth was not significantly inhibited from 5 to 12 days after pollination. In stress treatments with media water potentials of -1.6 or -2.0 megapascals, endosperm tissue accumulated water and solutes from 10 and 12 days after pollination at a rate similar to or greater than that of the control (-1.1 megapascals). In contrast, endosperm cell division was inhibited in all treatments relative to control. At 10 days after pollination, endosperm sucrose concentration was greater in two of the -2.0 megapascal treatments with 0.44 or 0.58 molar media sucrose compared to control kernels cultured in 0.29 molar sucrose at -1.1 megapascals. Significant increases in abscisic acid content per gram of fresh weight were detected in two -2.0 megapascal treatments (0.29 molar sucrose plus 0.29 molar sorbitol and 0.58 molar sucrose) at 10 days after pollination. We conclude that in cultured maize kernels, endosperm cell division was more responsive than fresh weight accumulation to low water potential treatments. Data were consistent with mechanisms involving abscisic acid or lowered tissue water potential, or an interaction of the two factors.also be considered (reviewed by Kermode [11]). Perhaps the effects of ABA observed on endosperm cell division could be duplicated or attenuated by altered solute concentration. In support of this possibility, studies have shown that the effects of ABA on embryo development can be mimicked to some extent by increased solute concentration (1,4,12). However, in some systems, such responses to solute concentration require a concomitant rise in endogenous ABA (21,25). Thus, to evaluate the effects of water deficit on endosperm development, both tissue water status (0', r, Op) and ABA levels need to be considered.In maize kemel culture, the level of carbohydrates available to the developing kemel is controlled by medium composition. In this study, we altered the i, of the medium by adding increments of sucrose and/or sorbitol. Sorbitol was used to impose the /w treatments in an attempt to separate the effects of increased carbohydrate nutrition from the effects of decreased 0,. Individual kemels with a small piece of subtending cob tissue were placed on the media and cultured for 3 to 7 d. This method excludes most matemal tissue, thereby decreasing import of ABA from leaves and roots. The goals of the present studies were to assess the extent to which endosperm cell division and FW accumulation respond to elevated osmoticum concentration and to evaluate the potential role of changes in tissue wat...

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Water Deficit in Pea Root Tips: Effects on the Cell Cycle and on the Production of Dehydrin-like Proteins

et al. 1997

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Dehydrin-like proteins have been detected in nuclei and cytoplasm of meristematic root tip cells from pea seedlings subjected to slow dehydration at 90 % relative humidity for 48 h or more. Evidence was gained from Western blotting and immunocytochemical experiments using an antibody raised against the conserved domain of dehydrin proteins. Flow cytometer analysis has shown that cycling cells of root tip meristems from dehydrated seedlings are mostly arrested in G2 phase. Other stress treatments thought to involve water depletion (osmotic stress, cold treatment) or to modulate cell response to water deficit (abscisic acid) gave less clear-cut results with all treatments lowering the proportion of cells entering the S phase, but without a definite and persistent arrest in any preferential phase of the cycle. Possible interrelationships between G2 arrest and dehydrin production are discussed.# 1997 Annals of Botany Company

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Abscisic Acid Inhibition of Endosperm Cell Division in Cultured Maize Kernels

Cited by 66 publications

References 26 publications

Intra-plant variation for progress of cell division in developing oat grains: a preliminary study

Intra-plant variation for progress of cell division in developing oat grains: a preliminary study

Endosperm Cell Division in Maize Kernels Cultured at Three Levels of Water Potential

Water Deficit in Pea Root Tips: Effects on the Cell Cycle and on the Production of Dehydrin-like Proteins

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