Water Deficit in Pea Root Tips: Effects on the Cell Cycle and on the Production of Dehydrin-like Proteins

Bracale, Marcella; Levi, Marisa; Cristian, Savini; Dicorato, Walter; Galli, Maria Grazia

doi:10.1006/anbo.1996.0356

Cited by 26 publications

(18 citation statements)

References 35 publications

(26 reference statements)

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“…However, they are primarily localized in the cytoplasm and nucleus. Studies have been done on maize embryos [27,50,53], developing tomato root primordia [36], pea root tip meristems subjected to slow dehydration [54], wheat crown tissues [34] and peach shoots [44]. Maize abscisic acid-responsive Rab17 (DHN1) protein, a YSK 2 -type, localizes to the nucleus and cytoplasm [27].…”

Section: Subcellular Localizationmentioning

confidence: 99%

Plant dehydrins — Tissue location, structure and function

Rorat

2006

Cellular and Molecular Biology Letters

304

228

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Dehydrins (DHNs) are part of a large group of highly hydrophilic proteins known as LEA (Late Embryogenesis Abundant). They were originally identified as group II of the LEA proteins. The distinctive feature of all DHNs is a conserved, lysine-rich 15-amino acid domain, EKKGIMDKIKEKLPG, named the K-segment. It is usually present near the C-terminus. Other typical dehydrin features are: a track of Ser residues (the S-segment); a consensus motif, T/VDEYGNP (the Y-segment), located near the N-terminus; and less conserved regions, usually rich in polar amino acids (the Φ-segments). They do not display a well-defined secondary structure. The number and order of the Y-, S-and K-segments define different DHN sub-classes: Y n SK n , Y n Kn, SKn, K n and K n S. Dehydrins are distributed in a wide range of organisms including the higher plants, algae, yeast and cyanobacteria. They accumulate late in embryogenesis, and in nearly all the vegetative tissues during normal growth conditions and in response to stress leading to cellular dehydration (e.g. drought, low temperature and salinity). DHNs are localized in different cell compartments, such as the cytosol, nucleus, mitochondria, vacuole, and the vicinity of the plasma membrane; however, they are primarily localized to the cytoplasm and nucleus. The precise function of dehydrins has not been established yet, but in vitro experiments revealed that some DHNs (YSK n -type) bind to lipid vesicles that contain acidic phospholipids, and others (K n S) were shown to bind metals and have the ability to scavenge hydroxyl radicals [Asghar, R. et al. Protoplasma 177 (1994)

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Section: Subcellular Localizationmentioning

confidence: 99%

Plant dehydrins — Tissue location, structure and function

Rorat

2006

Cellular and Molecular Biology Letters

304

228

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“…88 The decrease of mitotic activity following imposition of drought stress has been reported in root meristems. 89,90 The link with reduced CDK activity was provided by studies on wheat leaves where a reduction in mesophyll cell division was correlated with inhibition of CDKA1 activity. CDKA was phosphorylated on Tyr-15, a post-translational modification known to reduce kinase activity.…”

Section: Resultsmentioning

confidence: 99%

Cyclin dependent protein kinases and stress responses in plants

Kitsios

Doonan

2011

Plant Signaling & Behavior

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“…DHNs localize primarily to the nucleus or cytoplasm (Houde et al, 1995;Bracale et al, 1997), but studies also corroborate their presence either in the plasma membrane (Danyluk et al, 1998), endoplasmic reticulum (Neven et al, 1993), chloroplast (Wisniewski et al, 1999), mitochondria (Borovskii et al, 2002) or even in the vacuole (Heyen et al, 2002). DHNs may thus be expressed either constitutively [e.g.…”

Section: Structure and Functional Studies Of Dhnamentioning

confidence: 92%

“…Arabidopsis DHNX] or confined to the root tip (ERD14 and LTI29), vascular tissues (ERD14, LTI29 and RAB18) and stomatal guard cells (RAB18). DHNs accumulate within vascular tissues (Godoy et al, 1994;Houde et al, 1995;Danyluk et al, 1998;Nylander et al, 2001), pollen sacs, guard cells and root meristems (Nylander et al, 2001), endosperm and zygotic embryos of mature seeds, developing root primordia (Godoy et al, 1994;Bracale et al, 1997), wheat crown tissues (Houde et al, 1995) and peach shoots (Wisniewski et al, 1999). DHNs found only in the cytosol include those of C. plantagineum DSP16 (Schneider et al, 1993) and rice RAB21 (Mundy and Chua, 1988).…”

Section: Structure and Functional Studies Of Dhnamentioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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Water Deficit in Pea Root Tips: Effects on the Cell Cycle and on the Production of Dehydrin-like Proteins

Cited by 26 publications

References 35 publications

Plant dehydrins — Tissue location, structure and function

Plant dehydrins — Tissue location, structure and function

Cyclin dependent protein kinases and stress responses in plants

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

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