Effects on rat testes of the thiosemicarbazone derivative Schiff base (4-(1-phenylmethylcyclobutane-3-yl)-2-(2-hydroxybenzylidenehydrazino)thiazole) and its cadmium(II) complex

Abstract

The aim of this study was to investigate structural and biochemical changes in testes of rats treated with the thiosemicarbazone derivative thiazole ring Schiff base, (4-(1-phenyl-methylcyclobutane-3-yl)-2-(2-hydroxybenzylidene-hydrazino) thiazole (L), and its Cd(II) complex (CdL(2)). The animals were divided into three groups. Group I was designated as control. The rats in groups II and III were injected subcutaneously with L or CdL(2) respectively at 150-mg kg(-1) doses at 3-day intervals for 15 days. At the end of the study, blood samples were collected for biochemical analysis, and testes were removed for histological examinations. Serum levels of vitamin A, E and MDA of the L-injected group were similar to the control group. While CdL(2) treatment decreased serum vitamin A and E levels, it increased the MDA level compared to other groups. Histologically, the testes structures of L-treated animals were similar to the control. Spermatogenic cells in seminiferous tubules of CdL(2)-treated animals displayed necrosis. Nuclei of spermatogonia and primary spermatocytes were pyknotic and heterochromatic. Homogenous pink particles were present in place of the spermatids. The interstitial areas were oedematous and intertubular vessels were plugged. In conclusion, the present results indicate that L does not cause biochemical and morphological alterations, but its Cd(II) complex has degenerative effects in normal rat testes.

Antioxidant, pro‐oxidant effect of the thiosemicarbazone derivative Schiff base (4‐(1‐phenylmethylcyclobutane‐3‐yl)‐2‐(2‐hydroxybenzylidenehydrazino) thiazole) and its metal complexes on rats

“…The high level of MDA was observed in previous studies. 16,17 In addition, Cu(L) 2 treatment increased GSH-Px activity. It was reported that copper pyridoxal thiosemicarbazone (CuTSC) complex induced oxidative stress by generation of O .…”

“…The Cd(II) complex decreased serum vitamin A and E levels and increased the serum MDA level in rats. 17 In this study, all compounds were used at a dose of 25 mg kg À1 . As seen from the tables, LH did not affect serum vitamin A, E, C, MDA levels and erythrocyte GSH-Px activity, but Zn(L) 2 caused a significant increase in serum vitamin E which has not been previously described.…”

“…15,16 In a previous study, we investigated the effects on some serum biochemical parameters (vitamins A,E,C and MDA) and morphological changes in normal rat testes of the ligand 4-(1-phenyl-methylcyclobutane-3-yl)-2-(2-hydroxybenzylidenehydrazino)thiazole (LH) and its Cd(II) complex. 17 As far as we know there are very few studies related to antioxidant, pro-oxidant properties in vivo and morphological effects of these compounds in the literature. Moreover, only a few reports are available of its in vitro antioxidant activity.…”

Antiproliferative effects of some N‐benzylideneanilines

“…[2][3][4][5][6][7] Furthermore, Schiff bases are utilized as starting material in the synthesis of industrial and biological compounds such as b-lactams 8 and their metal complexes have an important pharmacological effect because of antineoplastic, antimalarial, antifungal, antibacterial, and anticancer features. [9][10] Previous studies indicate that Schiff bases, containing the >C --N-group, are known to have slight anticancer activities.…”

The Effect of the Serum Amino Acid Levels Thiosemicarbazone Derivatives and its Metal Complexes on Rats

“…However, only a few reports are available in vitro and in vivo antioxidative, pro-oxidative properties [17][18][19][20][21][22]. In previous studies, we have investigated the effects on the some serum biochemical parameters (vitamins A,E,C and MDA) and morphological changes normal rat testes, liver and kidney of the ligand 4-(1-phenyl-methylcyclobutane-3-yl)-2-(2-hydroxybenzylidenehydrazino)thiazole (LH) and its Cd(II), Zn(II) and Cu(II) complexes [17,22]. As far as we know there are no studies related to effects on the serum amino acid levels of these compounds in literature.…”