Effects of γ-synuclein on the tumorigenicity and metastasis of colon cancer SW1116 cells in vitro and in vivo

Abstract

Recent evidence suggests the involvement of γ-synuclein in tumorigenesis and tumor progression. The present study was designed to further clarify the effects of γ-synuclein on the biological features of colon cancer cells in vitro and in vivo. We constructed the eukaryotic expression vector and siRNA vector and selected stable transfectants to respectively upregulate and downregulate γ-synuclein expression in SW1116 cells. we found that silencing of γ-synuclein significantly attenuated SW1116 cell growth and colony formation in vitro (P<0.05), and overexpression of γ-synuclein moderately enhanced cell growth and colony formation, but not significantly when compared with the parental SW1116 cells and empty vector-transfected cells (P>0.05). Meanwhile, overexpression of γ-synuclein significantly facilitated SW1116 cell migration, invasion and adhesion to human liver sinusoidal endothelial cells (HLSECs) in vitro (P<0.05), and the effects were less attenuated by γ-synuclein knockdown (P>0.05). Furthermore, γ-synuclein promoted these malignant phenotypes in a γ-synuclein expression quantity-dependent manner not only in vitro but also in the in vivo expression. stable cells were injected subcutaneously into the right flank, and injected intrasplenically in nude mice. γ-synuclein knockdown suppressed the tumorigenicity of SW1116 cells in mice, which presented significantly smaller tumor masses on day 6 over a 30-day period, compared with empty vector cells (P<0.05). Meanwhile, overexpression of γ-synuclein led to a profound augmentation of liver metastasis in nude mice, not only in macroscopic appearance but also in the size and weight of livers (P<0.05). These results provide strong evidence that suggests γ-synuclein plays a positive role in the progression of colorectal cancer.

Exploring new biomarkers in the tumour microenvironment of canine inflammatory mammary tumours

“…49 Moreover, in a colon cancer cell line it has been demonstrated that induced SNCG overexpression increased cell migration, invasion and adhesion to endothelial cells. 50 The overexpression of SNCG we observed in CIMC could be related with the prominent role of cell migration and invasion in this tumour type.…”

“…In MDA‐MB‐231 mammary cancer cells, targeting SNCG with siRNA has been shown to reduce proliferation and migration dowregulating phosphorylation of ERK and AKT, and also induce apoptosis by cell cycle arrest . Moreover, in a colon cancer cell line it has been demonstrated that induced SNCG overexpression increased cell migration, invasion and adhesion to endothelial cells . The overexpression of SNCG we observed in CIMC could be related with the prominent role of cell migration and invasion in this tumour type.…”

γ-Synuclein confers both pro-invasive and doxorubicin-mediated pro-apoptotic properties to the colon adenocarcinoma LS 174T cell line

“…Although similar studies have been conducted previously, a concrete conclusion on the effects of γ-synuclein expression in colorectal cell lines still could not be drawn, due to the different cell lines used [11,24,25]. Besides, although considered raising from similar origin, different colorectal cancer cell lines have shown widely different patterns of growth and metastatic spread in the in vivo orthotopic mice model [26].…”

Synuclein-γ promotes migration of MCF7 breast cancer cells by activating extracellular-signal regulated kinase pathway and breaking cell-cell junctions

“…Their main differences are in their acidic C-terminal domain, which is presumably where their functional differences arise (24). Various studies have shown that SNCG promoted migration, invasion and metastasis of cancer cells (13)(14)(15)25), and SNCG was associated with recurrence and poor prognosis of multiple types of cancer. Our previous results also indicated that SNCG is an independent predictor for poor prognosis in patients with breast cancer and colon adenocarcinoma (8,11).…”