Effects of γ‐irradiation, storage and hydration on osteoinductivity of DBM and DBM/AM composite

Qiu, Qingqing; Connor, Jerome

doi:10.1002/jbm.a.31790

Cited by 20 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is well understood that the biological, chemical and mechanical properties of biomaterials are susceptible to change by diverse sterilization methods [12,15–18]. However, the contribution of the outermost molecular layer of an implant to its in vivo function is less well established.…”

Section: Introductionmentioning

confidence: 99%

Effect of cleaning and sterilization on titanium implant surface properties and cellular response

et al. 2012

View full text Add to dashboard Cite

Titanium (Ti) has been widely used as an implant material due to the excellent biocompatibility and corrosion resistance of its oxide surface. Biomaterials must be sterile before implantation, but the effects of sterilization on their surface properties have been less well studied. The effects of cleaning and sterilization on surface characteristics were bio-determined using contaminated and pure Ti substrata first manufactured to present two different surface structures: pretreated titanium (PT, Ra = 0.4 μm) (i.e. surfaces that were not modified by sandblasting and/or acid etching); (SLA, Ra = 3.4 μm). Previously cultured cells and associated extracellular matrix were removed from all bio-contaminated specimens by cleaning in a sonicator bath with a sequential acetone–isopropanol–ethanol–distilled water protocol. Cleaned specimens were sterilized with autoclave, gamma irradiation, oxygen plasma, or ultraviolet light. X-ray photoelectron spectroscopy (XPS), contact angle measurements, profilometry, and scanning electron microscopy were used to examine surface chemical components, hydrophilicity, roughness, and morphology, respectively. Small organic molecules present on contaminated Ti surfaces were removed with cleaning. XPS analysis confirmed that surface chemistry was altered by both cleaning and sterilization. Cleaning and sterilization affected hydrophobicity and roughness. These modified surface properties affected osteogenic differentiation of human MG63 osteoblast-like cells. Specifically, autoclaved SLA surfaces lost the characteristic increase in osteoblast differentiation seen on starting SLA surfaces, which was correlated with altered surface wettability and roughness. These data indicated that recleaned and resterilized Ti implant surfaces cannot be considered the same as the first surfaces in terms of surface properties and cell responses. Therefore, the reuse of Ti implants after resterilization may not result in the same tissue responses as found with never-before-implanted specimens.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of cleaning and sterilization on titanium implant surface properties and cellular response

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Irradiation of lyophilised DBM at low temperature has been reported to maintain the osteoinductive potential of the DBM (Wolfinbarger et al 2008;Qiu and Connor 2008).…”

Section: Terminal Sterilisationmentioning

confidence: 99%

Development of an improved bone washing and demineralisation process to produce large demineralised human cancellous bone sponges

2015

View full text Add to dashboard Cite

Shaped demineralised bone matrices (DBM) made from cancellous bone have important uses in orthopaedic and dental procedures, where the properties of the material allow its insertion into confined defects, therefore acting as a void filler and scaffold onto which new bone can form. The sponges are often small in size, <1.0 cm(3). In this study, we report on an improved bone washing and demineralisation process that allows production of larger DBM sponges (3.375 or 8.0 cm(3)) from deceased donor bone. These sponges were taken through a series of warm water washes, some with sonication, centrifugation, 100 % ethanol and two decontamination chemical washes and optimally demineralised using 0.5 N hydrochloric acid under vacuum. Demineralisation was confirmed by quantitative measurement of calcium and qualitatively by compression. Protein and DNA removal was also determined. The DBM sponges were freeze dried before terminal sterilisation with a target dose of 25 kGy gamma irradiation whilst frozen. Samples of the sponges were examined histologically for calcium, collagen and the presence of cells. The data indicated lack of cells, absence of bone marrow and a maximum of 1.5 % residual calcium.

show abstract

“…The osteoinductive properties of demineralized bone have been shown to be retained following sterilization with EO. 159,160 Despite the fact that irradiation causes polymer oxidation, chain scission, and the release of free radicals, the effect of irradiation on DBM osteoinductivity has mixed results. [156][157][158] When DBM was in hydrous state, it lost its osteoinductivity more easily than in anhydrous state.…”

Section: Demineralized Bone Matrixmentioning

confidence: 99%

Sterilization of Biomaterials of Synthetic and Biological Origin

Qiu¹,

Sun²,

Connor³

2011

Comprehensive Biomaterials

View full text Add to dashboard Cite

Effects of γ‐irradiation, storage and hydration on osteoinductivity of DBM and DBM/AM composite

Cited by 20 publications

References 21 publications

Effect of cleaning and sterilization on titanium implant surface properties and cellular response

Effect of cleaning and sterilization on titanium implant surface properties and cellular response

Development of an improved bone washing and demineralisation process to produce large demineralised human cancellous bone sponges

Sterilization of Biomaterials of Synthetic and Biological Origin

Contact Info

Product

Resources

About