Effects of yuja peel extract and its flavanones on osteopenia in ovariectomized rats and osteoblast differentiation

Jeon, Eun Joo; Lee, Da-Hye; Kim, Yang-Ji; Ahn, Jiyun; Kim, Min Jung; Hur, Jinyoung; Kim, Mina; Jang, Youngjin; Ha, Tae‐Youl; Seo, Dong-Hoan; Lee, Jong Suk; Sung, Mi Jeong; Jung, Chang Hwa

doi:10.1002/mnfr.201600257

Cited by 14 publications

(22 citation statements)

References 40 publications

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“…Taken together, the micro-CT analysis indicated that NM attenuated OVX-induced osteoporosis. Bone regeneration is related to an induction of osteoblast cell proliferation, ALP activation, intracellular Ca deposition, mineralization and extracellular matrix formation [49][50][51]. As shown in Figure 2A, 250-1000 µg/mL of NM increased ALP activity in a dose dependent manner.…”

Section: Discussionmentioning

confidence: 90%

“…The present study showed that NM treated group could increase ALP activity and gene expression of the osteogenic makers RUNX2, osteocalcin, BMP-2, COL-1, Wnt3a, and p-SMAD 1/5/8. These osteoblast-related markers are participated in bone regeneration, formation, and metabolism [51,54,55]. In addition, a previous study on the effects of genistein on osteoblast-associated metabolism proved that genes related to the BMP/SMAD signaling pathway showed the strongest enhancements in human bone marrow stromal cell (hBMSC) cultures [53].…”

Section: Discussionmentioning

confidence: 99%

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Effect of Nano-Montmorillonite on Osteoblast Differentiation, Mineral Density, and Osteoclast Differentiation in Bone Formation

Kim

Lee

et al. 2020

Nanomaterials

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Calcium-type montmorillonite, a phyllosilicate mineral, has diverse health benefits when introduced into the gastrointestinal tract or applied to the skin. However, the predominant use of this layered material has thus far been in traditional industries, despite its potential application in the pharmaceutical industry. We investigated the effects and mechanism of nano-montmorillonite (NM) on osteoblast and osteoclast differentiation in vivo and in vitro. We examined the osteogenic effects of NM with high calcium content (3.66 wt%) on alkaline phosphatase (ALP) activity, mineralization, bone microarchitecture, and expression level of osteoblast and osteoclast related genes in Ca-deficient ovariectomized (OVX) rats. Micro-computed tomography of OVX rats revealed that NM attenuated the low-Ca-associated changes in trabecular and cortical bone mineral density. It improved ALP activity and mineralization, as well as the expression of osteoblast and osteoclast differentiation associated genes. NM also activated the expression of runt-related transcription factor 2, osteocalcin, bone morphogenetic protein 2, and type 1 collagen via phosphorylated small mothers against decapentaplegic homolog 1/5/8 signaling. Further, NM repressed the expression of receptor activator for cathepsin K, nuclear factor kappa-B ligand and tartrate-resistant acid phosphatase. Therefore, NM inhibits osteoclastogenesis, stimulates osteoblastogenesis, and alleviates osteoporosis.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Effect of Nano-Montmorillonite on Osteoblast Differentiation, Mineral Density, and Osteoclast Differentiation in Bone Formation

Kim

Lee

et al. 2020

Nanomaterials

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“…ALP is an important indicator of bone mineralization, an early biomarker for osteopenia, and is expressed by all osteoblastic cells [24]. The differentiated osteogenic cells exhibited increased ALP activity and enhanced bone mineralization [222324]. The present study showed that melanin significantly increased ALP activities concentration-dependently (Fig.…”

Section: Discussionmentioning

confidence: 54%

“…Bone regeneration induced osteoblast proliferation, increased ALP activity, and stimulated intracellular calcium deposition, extracellular matrix formation, and mineralization [2223]. ALP is an important indicator of bone mineralization, an early biomarker for osteopenia, and is expressed by all osteoblastic cells [24]. The differentiated osteogenic cells exhibited increased ALP activity and enhanced bone mineralization [222324].…”

Section: Discussionmentioning

confidence: 99%

Melanin extract fromGallus gallus domesticuspromotes proliferation and differentiation of osteoblastic MG-63 cells via bone morphogenetic protein-2 signaling

et al. 2017

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BACKGROUND/OBJECTIVESGallus gallus domesticus (GD) is a natural mutant breed of chicken in Korea with an atypical characterization of melanin in its tissue. This study investigated the effects of melanin extracts of GD on osteoblast differentiation and inhibition of osteoclast formation.MATERIALS/METHODSThe effects of the melanin extract of GD on human osteoblast MG-63 cell differentiation were examined by evaluating cell viability, osteoblast differentiation, and expression of osteoblast-specific transcription factors such as bone morphogenetic protein 2 (BMP-2), small mothers against decapentaplegic homologs 5 (SMAD5), runt-related transcription factor 2 (RUNX2), osteocalcin and type 1 collagen (COL-1) by reverse transcription-polymerase chain reaction and western blotting analysis. We investigated the inhibitory effect of melanin on the osteoclasts formation through tartrate-resistant acid phosphatase (TRAP) activity and TRAP stains in Raw 264.7 cell.RESULTSThe melanin extract of GD was not cytotoxic to MG-63 cells at concentrations of 50-250 µg/mL. Alkaline phosphatase (ALP) activity and bone mineralization of melanin extract-treated cells increased in a dose-dependent manner from 50 to 250 µg/mL and were 149% and 129% at 250 µg/mL concentration, respectively (P < 0.05). The levels of BMP-2, osteocalcin, and COL-1 gene expression were significantly upregulated by 1.72-, 4.44-, and 2.12-fold in melanin-treated cells than in the control cells (P < 0.05). The levels of RUNX2 and SMAD5 proteins were higher in melanin-treated cells than in control vehicle-treated cells. The melanin extract attenuated the formation of receptor activator of nuclear factor kappa-B ligand-induced TRAP-positive multinucleated RAW 264.7 cells by 22%, and was 77% cytotoxic to RAW 264.7 macrophages at a concentration of 500 µg/mL.CONCLUSIONSThis study provides evidence that the melanin extract promoted osteoblast differentiation by activating BMP/SMADs/RUNX2 signaling and regulating transcription of osteogenic genes such as ALP, type I collagen, and osteocalcin. These results suggest that the effective osteoblastic differentiation induced by melanin extract from GD makes it potentially useful in maintaining bone health.

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“…The experimental AAB group was administrated orally with 100 mg/kg of AAB daily. This dose of AAB was selected based on previous references regarding the effect of the medicinal herb on osteoporosis (17)(18)(19). The E2 group was intraperitoneally injected with 10 µg/kg E2 daily.…”

Section: Methodsmentioning

confidence: 99%

Anemarrhena�asphodeloides Bunge ameliorates osteoporosis by suppressing osteoclastogenesis

et al. 2018

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Anemarrhena asphodeloides Bunge has been traditionally used in Korean medicine for its antipyretic, diuretic, sedative, and antitussive effects. In the present study, the effects of an ethanol extract of A. asphodeloides Bunge (AAB) on osteoporosis and its underlying mechanisms on bone remodeling were investigated. Osteoporosis was induced in IcR strain mice by ovariectomy. The mice were divided into four groups: sham, ovariectomized, 17β-estradiol and 100 mg/kg AAB. The treatment was continued for 4 weeks. Bone mineral density (BMd) and bone mineral content (BMc) were measured using dual-energy X-ray absorptiometry. In addition, Raw 264.7 cells were treated in the presence of 0.1, 1 and 10 µg/ml AAB with 100 ng/ml receptor activator of nuclear factor κΒ ligand (RANKL) to induce osteoclast formation and stained with tartrate resistant acid phosphatase. In addition, levels of osteoclast-related factors were analyzed to investigate the signaling cascades in osteoclasts. The results demonstrated that AAB treatment reversed the decreases of both BMd and BMc in osteoporotic femurs. Additionally, the formation of osteoclasts was significantly suppressed by the AAB treatment in RANKL-stimulated Raw 264.7 cells. compared with cells treated with RANKL alone, the AAB-treated osteoclasts had significantly decreased tumor necrosis factor-α and interleukin-6. The protein levels of c-fos were also decreased in the AAB-treated osteoclasts. Furthermore, the RANKL-induced nuclear translocation of nuclear factor-κB was attenuated in osteoclasts by the AAB treatment compared with cells treated with RANKL alone. Finally, AAB treatment downregulated the phosphorylation of mitogen-activated protein kinases. The present results demonstrated that AAB exhibited ameliorative effects on osteoporosis by inhibiting osteoclastogenesis, and suggested that AAB may be a potential candidate for the treatment of osteoporosis.

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Effects of yuja peel extract and its flavanones on osteopenia in ovariectomized rats and osteoblast differentiation

Cited by 14 publications

References 40 publications

Effect of Nano-Montmorillonite on Osteoblast Differentiation, Mineral Density, and Osteoclast Differentiation in Bone Formation

Effect of Nano-Montmorillonite on Osteoblast Differentiation, Mineral Density, and Osteoclast Differentiation in Bone Formation

Melanin extract fromGallus gallus domesticuspromotes proliferation and differentiation of osteoblastic MG-63 cells via bone morphogenetic protein-2 signaling

Anemarrhena�asphodeloides Bunge ameliorates osteoporosis by suppressing osteoclastogenesis

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