Effects of wounding on glucosinolates in the cotyledons of oilseed rape and mustard

Bodnaryk, Robert P.

doi:10.1016/0031-9422(92)83609-3

Cited by 189 publications

(147 citation statements)

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“…All these mechanisms appear to act independently of glucosinolates (Bodnaryk 1991(Bodnaryk , 1992. In this study, lines of S. alba differing by 1000-fold in their sinalbin concentration in cotyledons showed the same level of antixenosis against flea beetles, proving that this resistance is independent of glucosinolates.…”

Section: Discussionsupporting

confidence: 56%

“…The cotyledons of S. alba have multiple resistance mechanisms, including tolerance (Bodnaryk and Lamb 1991;Brandt and Lamb 1993), constitutive antixenosis (Bodnaryk and Lamb 1991) and wound-inducible resistance (Bodnaryk 1992;Palaniswamy and Lamb 1993). All these mechanisms appear to act independently of glucosinolates (Bodnaryk 1991(Bodnaryk , 1992.…”

Section: Discussionmentioning

confidence: 99%

“…Glucosinolates in partially purified extracts were bound to a column of DEAESephadex A-25 and desulphated with arylsulfatase (Sigma). The desulphoglucosinolates were eluted from the column with water and separated by HPLC on a 250-mm LiChrosphere 100 RP-19 (10 µm) column (Merck) with monitoring at 227 nm and quantitation by comparison of peak areas with an internal standard of O-nitrophenyl-beta-D-galactopyranoside (Sigma) (Bodnaryk and Palaniswamy 1990;Bodnaryk 1992).…”

Section: Extraction and Quantitation Of Glucosinolatesmentioning

confidence: 99%

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Will low-glucosinolate cultivars of the mustards Brassica juncea and Sinapis alba be vulnerable to insect pests?

Bodnaryk¹

1997

Can. J. Plant Sci.

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Will low-glucosinolate cultivars of the mustards Brassica juncea and Sinapis alba be vulnerable to insect pests? Can J. Plant Sci. 77: 283-287. Foliar concentrations of the predominant glucosinolates in the mustards B. juncea (allyl glucosinolate, sinigrin) and S. alba (p-hydroxybenzyl glucosinolate, sinalbin) were determined in lines that had been selected in breeding programs for low levels of glucosinolates in their seeds for the oilseed market. The glucosinolate concentrations found in the cotyledons and leaves of the selected lines were also low, often by three or more orders of magnitude, compared with the unselected parent. The flea beetle, Phyllotreta cruciferae Goeze, and the diamondback moth, Plutella xylostella (L.), (both crucifer specialists) fed at equal rates on B. juncea and its low-glucosinolate lines, indicating that these species are insensitive to sinigrin and suggesting that their pest status on low-glucosinolate lines of B. juncea will likely remain unchanged. By contrast, the bertha armyworm, Mamestra configurata Wlk. (a generalist) fed up to fivefold more on the leaves of low-glucosinolate lines, suggesting that its pest status on low-glucosinolate B. juncea is likely to worsen relative to the mustard B. juncea. Flea beetles, diamondback moth, and the bertha armyworm fed at equal rates on the mustard S. alba and a low-glucosinolate line indicating that the status of these pests on low-glucosinolate S. alba will unlikely be much different from the mustard S. alba.Lines of S. alba differing in their sinalbin concentration by 1000-fold had equal levels of antixenosis resistance in their cotyledons against flea beetles, leaving little doubt that this resistance is independent of glucosinolates. Flea beetle resistance was also detected in the cotyledons of a low-glucosinolate line of B. juncea. The resistance was not detected in the parental line or in another low-glucosinolate line and appears to have arisen fortuitously during crossing and selection. Since resistance occurs in a line with concentrations of sinigrin three or more orders of magnitude lower than non-resistant lines, this resistance is also unlikely to be glucosinolate based. The mustards S. alba and B. juncea are a useful source of cotyledon resistance against flea beetles that is compatible with the aims of oilseed breeding. , montrant que les deux espèces sont indifférentes à la présence de la sinigrine et que, par conséquent, elles conserveront vraisemblablement la même visibilité sur les lignées p.g. En revanche, la légionnaire bertha Mamestra configurata Wlk. (plus polyphage) se nourrissait jusqu'à cinq fois plus à même les feuilles des lignées à basse teneur en glucosinolates, ce qui laisse à penser que sa nuisibilité risque d'empirer sur les lignées de ce type, par rapport avec l'espèce d'origine chez Sinapis alba, le degré d'activité des altises, de la fausse-teigne et de la légion-naire bertha était sensiblement équivalent sur l'espèce non sélectionnée et sur sa lignée p.g., montrant que le degré de nuisibilité sur les...

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Extraction and Quantitation Of Glucosinolatesmentioning

confidence: 99%

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Will low-glucosinolate cultivars of the mustards Brassica juncea and Sinapis alba be vulnerable to insect pests?

Bodnaryk¹

1997

Can. J. Plant Sci.

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“…These different accumulation patterns suggest the existence of different regulatory mechanisms for their synthesis or different metabolic fluxes as well as different physiological roles during senescence in plants. Reports suggesting that indole-GLSs, especially 1MI3M and 4MI3M, were more responsive to induction than Met-GLSs by wounding, insects, and JAs (Bodnaryk, 1992(Bodnaryk, , 1994Doughty et al, 1995) fit with the high accumulation of 1MI3M and 4MI3M in senescent leaves, where cellular damage and JA accumulation occur (BuchananWollaston et al, 2003). Although the temporal accumulation patterns of Met-GLSs and indole-GLSs were different, they were similarly distributed within a single leaf.…”

Section: Sulfur-containing Secondary Metabolitesmentioning

confidence: 96%

Comprehensive Dissection of Spatiotemporal Metabolic Shifts in Primary, Secondary, and Lipid Metabolism during Developmental Senescence in Arabidopsis

et al. 2013

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Developmental senescence is a coordinated physiological process in plants and is critical for nutrient redistribution from senescing leaves to newly formed sink organs, including young leaves and developing seeds. Progress has been made concerning the genes involved and the regulatory networks controlling senescence. The resulting complex metabolome changes during senescence have not been investigated in detail yet. Therefore, we conducted a comprehensive profiling of metabolites, including pigments, lipids, sugars, amino acids, organic acids, nutrient ions, and secondary metabolites, and determined approximately 260 metabolites at distinct stages in leaves and siliques during senescence in Arabidopsis (Arabidopsis thaliana). This provided an extensive catalog of metabolites and their spatiotemporal cobehavior with progressing senescence. Comparison with silique data provides clues to source-sink relations. Furthermore, we analyzed the metabolite distribution within single leaves along the basipetal sink-source transition trajectory during senescence. Ceramides, lysolipids, aromatic amino acids, branched chain amino acids, and stressinduced amino acids accumulated, and an imbalance of asparagine/aspartate, glutamate/glutamine, and nutrient ions in the tip region of leaves was detected. Furthermore, the spatiotemporal distribution of tricarboxylic acid cycle intermediates was already changed in the presenescent leaves, and glucosinolates, raffinose, and galactinol accumulated in the base region of leaves with preceding senescence. These results are discussed in the context of current models of the metabolic shifts occurring during developmental and environmentally induced senescence. As senescence processes are correlated to crop yield, the metabolome data and the approach provided here can serve as a blueprint for the analysis of traits and conditions linking crop yield and senescence.

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“…Several isoforms of myrosinase enzymes occur, which are regulated in organ-specific and developmental-stage-specific manners (Falk et al, 1992;James and Rossiter, 1991;Lenman et al, 1993). Although already formed, the myrosinaseiglucosinolate system is subject to alterations by stresses, such as wounding (Bodnaryk, 1992;Koritsas et al, 1991), pests (Koritsas et al, 1991) and pathogens (Doughty et al, 1991;Shattuck, 1993), and by signalling molecules, such as methyl jasmonate (MeJA ; Bodnaryk, 1994;Doughty et al, 1995) and salicylic acid (SA; Kiddle et al, 1994). Attempts have been made to detect changes in the formation of hydrolysis products in seeds from diseased B m ssicu cumpestris plants compared with healthy plants, but without success (Shattuck, 1993).…”

mentioning

confidence: 99%

The Myrosinase‐Binding Protein from Brassica Napus Seeds Possesses lectin Activity and has a Highly Similar Vegetatively Expressed Wound‐Inducible Counterpart

Taipalensuu

Eriksson

Rask

1997

European Journal of Biochemistry

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This communication demonstrates that proteins in the family of myrosinase-binding proteins (MBP) present in seeds of Brassicu napus possess lectin activity, binding most efficiently to p-aminophenyl a-D-mannopyranoside-agarose, and to some extent to N-acetylglucosamine-agarose. A cDNA encoding a vegetatively expressed, wound-inducible counterpart to these seed MBP was isolated and characterised. Upon wounding, this MBP transcript accumulated in old and young leaves, and was systemically expressed in the young plant. Additionally, the wound-induced MBP transcript increased in abundance after treating the young plants with methyl jasmonate (MeJA), jasmonic acid (JA) or abscisic acid (ABA), and to some extent in response to the ethylene precursor 1 -aminocyclopropane-1-carboxylic acid. Expression induced by wounding, ABA or JA was antagonised by simultaneous feeding of the plants with salicylic acid. MBP polypeptides accumulated in MeJA-treated plants. The myrosinases redistributed from the soluble fraction into the insoluble fraction of a tissue extract after induction. The most abundant MBP (94 kDa) partitioned in the insoluble fraction, while two larger MBP (103 kDa and 108 kDa) were present only in the soluble fraction of extracts obtained from the control or MeJA-treated plant tissues.

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Effects of wounding on glucosinolates in the cotyledons of oilseed rape and mustard

Cited by 189 publications

References 22 publications

Will low-glucosinolate cultivars of the mustards Brassica juncea and Sinapis alba be vulnerable to insect pests?

Will low-glucosinolate cultivars of the mustards Brassica juncea and Sinapis alba be vulnerable to insect pests?

Comprehensive Dissection of Spatiotemporal Metabolic Shifts in Primary, Secondary, and Lipid Metabolism during Developmental Senescence in Arabidopsis

The Myrosinase‐Binding Protein from Brassica Napus Seeds Possesses lectin Activity and has a Highly Similar Vegetatively Expressed Wound‐Inducible Counterpart

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