Effects of Wollastonite on Proliferation and Differentiation of Human Bone Marrow-derived Stromal Cells in PHBV/Wollastonite Composite Scaffolds

Abstract

In this study, the effects of wollastonite on proliferation and differentiation of human bone marrow-derived stromal cells (hBMSCs) have been investigated based on a polyhydroxybutyrate-co-hydroxyvalerate (PHBV)/ wollastonite (W) composite scaffolds system. Cell morphology, proliferation, and differentiation were measured. The results showed that the incorporation of wollastonite benefited hBMSCs adhesion, proliferation, and differentiation rate. In addition, an increase of proliferation and differentiation rate was observed when the wollastonite content in the PHBV/W composite scaffolds increased from 10 to 20 wt%. Based on our previous studies on PHBV/W composite discs, the differentiation measurements in this paper further proved that the wollastonite itself can stimulate the hBMSCs to differentiate toward osteoblasts without any osteogenic medium, and the ionic products (Ca and Si) released from wollastonite might contribute to this advantage. It is also suggested that the osteogenic differentiation of the hBMSCs can be affected by adjusting the wollastonite content in the composite scaffolds.

Ion Therapy: A Novel Strategy for Acute Myocardial Infarction

“…[43,44] At room temperature, by continuously mixing aqueous solution of Na 2 SiO 3 (1 mol L −1 ) and Ca (NO3) 2 (1 mol L −1 ) for 24 h, the reaction mixture with a molar mass of Na 2 SiO 3 :Ca (NO 3 ) 2 = 1:1 was obtained. [43,44] At room temperature, by continuously mixing aqueous solution of Na 2 SiO 3 (1 mol L −1 ) and Ca (NO3) 2 (1 mol L −1 ) for 24 h, the reaction mixture with a molar mass of Na 2 SiO 3 :Ca (NO 3 ) 2 = 1:1 was obtained.…”

Osseointegration of nanohydroxyapatite- or nano-calcium silicate-incorporated polyetheretherketone bioactive composites in vivo

“…Synthetic HA material is biocompatible and bioactive and is clinically used as an important bone substitute. 11 Some studies have proved that CS is biocompatible, biodegradable, and bioactive, with the ability to stimulate proliferation and osteogenic differentiation of osteoblasts, [12][13][14][15][16] and CS has been shown to have higher bioactivity than calcium phosphate materials. [17][18][19] Abu Bakar et al 20 prepared 20 volume% (vol%) HA-reinforced PEEK composite (HA/PEEK) with a porosity of 60% and pore size ranging from 300 to 600 mm using a leaching of particulate technique employing a suitable pore-forming agent, and these materials were implanted into the distal metaphyseal femur of pigs to evaluate the biological responses and tissue ingrowth of the material.…”

Scaffolds containing chitosan/carboxymethyl cellulose/mesoporous wollastonite for bone tissue engineering

“…The cells were harvested at different time periods (2 h, 4 h, 8 h and 24 h). Total RNA was isolated using TRIzol reagent, subjected to cDNA synthesis and followed by real time PCR analysis as described previously [19]. The primers used in the reaction for pre-mir-15b were: Sense: 5 -GGCCTTAAAGTACTGTAGCAGC-3 ; Antisense: 5 -CCTTAAATTTCTAGAGCAGC-3 ; RPL13a (internal control): Sense: 5 -AAGTACCAGGCAGTGACAG-3 ; Antisense: 5 -CCTGTTTCCGTAGCCTCATG-3 .…”

“…CS/CMC scaffolds containing bioactive glass have also been reported for bone tissue engineering applications [17]. Wollastonite (WS) or calcium silicate is a silicon based ceramic particle and is biocompatible, biodegradable and bioactive in nature [18][19][20]. Mesoporous particles are found to possess high specific surface area and pore volume which significantly improved the kinetic process of apatite formation on their surface thereby exhibiting better bioactivity [21,22] Biomaterial interaction with cell could activate intracellular signaling components/regulators resulting in osteogenesis.…”