Effects of Water Availability in the Soil on Tropane Alkaloid Production in Cultivated Datura stramonium

Abstract

Background: different Solanaceae and Erythroxylaceae species produce tropane alkaloids. These alkaloids are the starting material in the production of different pharmaceuticals. The commercial demand for tropane alkaloids is covered by extracting them from cultivated plants. Datura stramonium is cultivated under greenhouse conditions as a source of tropane alkaloids. Here we investigate the effect of different levels of water availability in the soil on the production of tropane alkaloids by D. stramonium. Methods: We tested four irrigation levels on the accumulation of tropane alkaloids. We analyzed the profile of tropane alkaloids using an untargeted liquid chromatography/mass spectrometry method. Results: Using a combination of informatics and manual interpretation of mass spectra, we generated several structure hypotheses for signals in D. stramonium extracts that we assign as putative tropane alkaloids. Quantitation of mass spectrometry signals for our structure hypotheses across different anatomical organs allowed us to identify patterns of tropane alkaloids associated with different levels of irrigation. Furthermore, we identified anatomic partitioning of tropane alkaloid isomers with pharmaceutical applications. Conclusions: Our results show that soil water availability is an effective method for maximizing the production of specific tropane alkaloids for industrial applications.

Induced Local and Systemic Defense Responses in Tomato Underlying Interactions Between the Root-Knot Nematode Meloidogyne incognita and the Potato Aphid Macrosiphum euphorbiae

“…The separation and characterization of secondary metabolites were done according to Mbaluto et al (2020) . The data was processed in MS-DIAL (v. 4.00, RIKEN) according to Moreno-Pedraza et al (2019) and with modification of several parameters including retention time-end = 12.5 min, mass range end = 1,500 mass to charge ratio ( m/z ), and the alignment parameter setting: retention time tolerance = 0.2 min. We generated two datasets (i.e., leaves and roots datasets) from which we selected all features with mass to charge ratio ( m/z ) of 576.3 and 578.4 at retention time 11–12 min for each study time point.…”

Comparative Screening for Determination of Atropine and Scopolamine in Different Species of Datura in Iraq and Iran

“…These compounds work as anticholinergic agent's affecting the central and peripheral nervous system a competitive, non-selective muscarinic acetylcholine receptor antagonists that prevent the binding of the physiological neurotransmitter acetylcholine. The alkaloids atropine, scopolamine, and their derivatives are the active ingredients in numerous pharmaceuticals ranging from antidotes for poisoning by organophosphorous compounds, antispasmodics, anti-motion sickness agents, and antiemetics (Moreno-Pedraza et al, 2019).…”

Alkaloids of the Genus Datura: Review of a Rich Resource for Natural Product Discovery

“…Profiling of Datura alkaloids using LC separations coupled to mass spectrometry (LC-MS) has been performed on a variety of mass spectrometry platforms including triple-quadrupole [10], ion trap [65], hybrid triple-quad/linear ion trap [36], time-of-flight (ToF) [75], quadrupole time-of-flight (QToF) hybrid, or orbitrap instruments [32,36,77,85]. Ionization usually has involved electrospray ionization (ESI), although APCI (atmospheric pressure chemical ionization) has also been used [16,84].…”

“…Extractions of alkaloids from Datura plant tissues have been performed on fresh, flash-frozen, or dried Datura tissue. Tissue is often macerated or powdered (sometimes mechanically, such as the ball-milling technique used by Moreno-Pedraza [ 32 ]), and then treated with an appropriate solvent to extract the alkaloids. Most solvents described in the literature are relatively non-reactive (i.e., will not degrade the components or significantly change the chemical composition of the sample).…”