Effects of water activity, leucine and thiamine on production of aroma compounds by Ceratocystis fimbriata

Sánchez, Leonora; Sánchez, Fausto; Christen, Pierre; Revah, Sergio

doi:10.1023/b:wibi.0000021751.82008.30

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…Firstly, it shows that Ceratocystis spp. grown in vitro emit a unique blend of VOC (Sánchez, Sánchez, Christen, & Revah, 2004) that clearly differentiate this species from other fungal pathogens. Emission of specific VOC by Ceratocystis spp.…”

Section: Discussionmentioning

confidence: 99%

Volatile organic compounds (VOC) as biomarkers for detection of Ceratocystis platani

et al. 2020

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Ceratocystis platani causes canker stain of plane trees, and it represents a serious disease of Platanus spp. both in the United States and Europe. Current chemical or biological controls do not effectively manage C. platani, so new preventive methods need to be developed in order to limit this pathogen spreading. In this work, we have characterized the main volatile organic compounds (VOC) emitted in vitro from pure cultures of C. platani and other common pathogenic fungal species of hosts plants growing in the same ecosystems as plane trees. We found that C. platani emitted a similar blend of VOC compared with phylogenetically similar species C. populicola. In particular, C. platani was characterized by emission of isoamyl acetate and isobutyl acetate while C. populicola by ethyl acetate and isobutyl acetate, which were not released by any of the other out‐group fungal species grown on the same medium. Moreover, following a targeted approach based on the main VOC found in vitro, we have successfully validated in vivo that VOC uniquely emitted by C. platani (i.e. isobutyl acetate along with isoamyl alcohol) were released from the bark of plane trees following C. platani inoculation. Our results highlight the possibility to exploit VOC emitted specifically by C. platani as biomarkers to recognize Platanus x acerifolia plants infected by this pathogen.

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Section: Discussionmentioning

confidence: 99%

Volatile organic compounds (VOC) as biomarkers for detection of Ceratocystis platani

et al. 2020

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“…Since Ceratocystis species are known to produce a wide range of volatile compounds (Lanza and Palmer, 1977;Sanchez et al, 2002;Sprecher and Hanssen, 1983), we attempted to purify the active compound through distillation (Fig S1A) and tested the resulting fractions on zebrafish embryos. The most potent fraction was analyzed first by LC-MS revealing a distinct fragmentation pattern specific for the active compound at m/z = 137.2, 95.1 and 81.1 (Fig.…”

Section: Monoterpenes Cause Ectopic Tail Formation In Zebrafish Embryosmentioning

confidence: 99%

Migration of Kupffer’s vesicle derived cells is essential for tail morphogenesis in zebrafish embryos

Hoeksma,

Hertog

2024

Preprint

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A phenotypic screen of fungal filtrates on developing zebrafish embryos identified metabolites from the fungusCeratocystis populicolato induce ectopic tail formation, including a split notochord and a duplicated caudal fin. Chemical analyses led to the identification of monoterpene alcohols, in particular geraniol, as active compounds inducing ectopic tail formation during a specific 4 h time window during tail bud stage. Embryos from Tüpfel long fin zebrafish (TL) were more susceptible to ectopic tail formation by geraniol than embryos from Wild Indian Karyotpe (WIK) zebrafish, indicating zebrafish strain specificity. RNA sequencing on tail buds of 15-somite stage embryos revealed downregulation of essential genes of the retinoic acid signaling pathway and differential expression ofcyp26a1andfgf8aand downstreamhox-genes was validated. Time-lapse imaging revealed that Kupffer’s vesicle derived cells failed to migrate shorty after Kupffer’s vesicle collapse upon geraniol treatment and these cells failed to merge with progenitors from the tail bud. Instead, these cells contributed to an ectopic tail, expressing markers for presomitic mesoderm, somite and notochord tissue. Taken together, our data suggests that Kupffer’s vesicle cells harbor tail progenitor capacity, and proper migration of these cells is essential for normal tail morphogenesis.Summary StatementInhibition of Kupffer’s vesicle derived cell migration affected tail morphogenesis and resulted in ectopic tail formation in zebrafish embryos.

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Current awareness in flavour and fragrance

2004

Flavour & Fragrance J

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Effects of water activity, leucine and thiamine on production of aroma compounds by Ceratocystis fimbriata

Cited by 3 publications

References 28 publications

Volatile organic compounds (VOC) as biomarkers for detection of Ceratocystis platani

Volatile organic compounds (VOC) as biomarkers for detection of Ceratocystis platani

Migration of Kupffer’s vesicle derived cells is essential for tail morphogenesis in zebrafish embryos

Current awareness in flavour and fragrance

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