Effects of Vitrification on Outcomes of In VivoMature, In Vitro-Mature and Immature Human Oocytes

Song, Wenyan; Peng, Zhaoliang; Jin, Haixia; Yao, Guidong; Sheng, Shi; Yang, Hongyi; Zhang, Xiangyang; Sun, Yingpu

doi:10.1159/000445564

Cited by 19 publications

(9 citation statements)

References 29 publications

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“…Song et al (106) were also using GV- and GVBD (MI)-stage oocytes generated from IVF cycles. Control was oocyte matured in vivo without vitrification and vitrification groups were where vitrification was performed before or after IVM of immature oocytes.…”

Section: Methodsmentioning

confidence: 99%

Immature Oocyte for Fertility Preservation

et al. 2019

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In vitro maturation (IVM) of human immature oocytes has been offered to women who are at risk of developing ovarian hyperstimulation syndrome (OHSS) caused by gonadotropin stimulation, such as PCO(S) patients or who have poor ovarian reserve. Cryopreservation of oocytes matured in vivo obtained in IVF cycles has improved after implementing the vitrification method and many successful results have been reported. Now, this procedure can be successfully offered to fertility preservation programs for patients who are in danger of losing their ovarian function due to medical or social reasons, and to oocyte donation programs. This vitrification technique has also been applied to cryopreserve oocytes obtained from IVM program. Some advantages of oocytes vitrification related with IVM are: (1) eliminating costly drugs and frequent monitoring; (2) completing treatment within 2 to 10 days (3) avoiding the use of hormones in cancer patients with hormone-sensitive tumors; and (4) retrieving oocytes at any point in menstrual cycle, even in the luteal phase. In addition, immature oocytes can also be collected from extracorporeal ovarian biopsy specimens or ovaries during caesarian section. Theoretically, there are two possible approaches for preserving immature oocytes: oocyte cryopreservation at the mature stage (after IVM) and oocyte cryopreservation at the Germinal Vesicle (GV)-stage (before IVM). Both vitrification of immature oocyte before/after IVM is not currently satisfactory. Nevertheless, many IVF centers worldwide are doing IVM oocyte cryopreservation as one of the options to preserve fertility for female cancer. Therefore, more studies are urgently required to improve IVM- and vitrification method to successfully preserve oocytes collected from cancer patients. In this review, present oocyte maturation mechanisms and recent progress of human IVM cycles will be discussed first, followed by some studies of the vitrification of human IVM oocyte.

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Section: Methodsmentioning

confidence: 99%

Immature Oocyte for Fertility Preservation

et al. 2019

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“…Although the cryopreservation technology for human oocytes has been enhanced and has achieved a high survival rate, it still cannot be routinely applied in clinical practice because of the poor developmental competence of oocytes after cryopreservation 11,12 . A previous study has shown that MLT acts as a protective agent that can promote oocyte development 58 .…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, although oocyte cryopreservation technology has been optimized and has achieved a high survival rate after cryopreservation, the blastocyst rate and the clinical pregnancy rate of cryopreserved oocytes are still lower than those of the fresh oocytes. Recent studies have shown that the blastocyst rate is 3.5%‐21.4% 10‐12 and the clinical pregnancy rate is 10.7% 13 of in vitro‐matured metaphase II (IVM‐MII) human oocytes after cryopreservation. Therefore, it is crucial to improve the quality of vitrified‐warmed oocytes.…”

Section: Introductionmentioning

confidence: 99%

Melatonin improves the effect of cryopreservation on human oocytes by suppressing oxidative stress and maintaining the permeability of the oolemma

Zhang

Ding

et al. 2020

Journal of Pineal Research

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Cryopreservation causes cryoinjury to oocytes and impairs their developmental competence. Melatonin (MLT) can improve the effect of cryopreservation in animal oocytes. However, no such studies on human oocytes have been reported. In this study, collected in vitro‐matured human oocytes were randomly divided into the following groups: fresh group, MLT‐treated cryopreservation (MC) group, and no‐MLT‐treated cryopreservation (NC) group. After vitrification and warming, viable oocytes from these three groups were assessed for their mitochondrial function, ultrastructure, permeability of oolemma, early apoptosis, developmental competence, and cryotolerance‐related gene expression. First, fluorescence staining results revealed that oocytes from the 10−9 M subgroup showed the lowest intracellular reactive oxygen species and Ca2+ levels and highest mitochondrial membrane potential among the MC subgroups (10−11, 10−9, 10−7, and 10−5 M). In subsequent experiments, oocytes from the 10−9 M‐MC group were observed to maintain the normal ultrastructural features and the permeability of the oolemma. Compared with those of the oocytes in the NC group, the early apoptosis rate significantly decreased (P < .01), whereas both the high‐quality cleavage embryo and blastocyst rates significantly increased (both P < .05) in the oocytes of the 10−9 M‐MC group. Finally, single‐cell RNA sequencing and immunofluorescence results revealed that aquaporin (AQP) 1/2/11 gene expression and AQP1 protein expression were upregulated in the MC group. Therefore, these results suggest that MLT can improve the effect of cryopreservation on human oocytes by suppressing oxidative stress and maintaining the permeability of the oolemma.

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“…A prospective cohort study showed that an increase in the perivitelline gap as well as a thickening and hardening of the zona pellucida was observed in oocytes after vitrification freezing [ 35 ]. Also, reduced ATP content and inadequate mitochondrial energy can lead to impaired spindle assembly and decreased spindle density [ 36 , 37 ]. The stability of the MMP and calcium level is particularly important for the quality of the oocyte.…”

Section: Melatonin Benefits Vitrification Freezingmentioning

confidence: 99%

“…And these excess lipid peroxides produce excess MDA, which leads to DNA damage even apoptosis in oocytes [ 67 ]. Higher levels of oxidative stress may also lead to spindle disorders, organelle degeneration, cytoplasmic heterogeneity, and reduced mitochondrial function in oocytes [ 36 , 39 , 40 , 68 ]. And ultimately, it results in poorer fertilization rates, lower high-quality blastocyst rates, and worse clinical pregnancy outcomes in PCOS patients [ 69 ].…”

Section: Melatonin Improves the Dysregulation Of Oxidative Stress Homeostasis Associated With Pcosmentioning

confidence: 99%

Applications of Melatonin in Female Reproduction in the Context of Oxidative Stress

Jiang

Shi

Liu

et al. 2021

Oxidative Medicine and Cellular Longevity

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Oxidative stress has been recognized as one of the causal mediators of female infertility by affecting the oocyte quality and early embryo development. Improving oxidative stress is essential for reproductive health. Melatonin, a self-secreted antioxidant, has a wide range of effects by improving mitochondrial function and reducing the damage of reactive oxygen species (ROS). This minireview illustrates the applications of melatonin in reproduction from four aspects: physiological ovarian aging, vitrification freezing, in vitro maturation (IVM), and oxidative stress homeostasis imbalance associated with polycystic ovary syndrome (PCOS), emphasising the role of melatonin in improving the quality of oocytes in assisted reproduction and other adverse conditions.

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Effects of Vitrification on Outcomes of In VivoMature, In Vitro-Mature and Immature Human Oocytes

Cited by 19 publications

References 29 publications

Immature Oocyte for Fertility Preservation

Immature Oocyte for Fertility Preservation

Melatonin improves the effect of cryopreservation on human oocytes by suppressing oxidative stress and maintaining the permeability of the oolemma

Applications of Melatonin in Female Reproduction in the Context of Oxidative Stress

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