Effects of Vitis vinifera L. leaves extract on UV radiation damage in human keratinocytes (HaCaT)

Marabini, Laura; Melzi, Gloria; Lolli, Federica; Dell’Agli, Mario; Piazza, Stefano; Sangiovanni, Enrico; Marinovich, Marina

doi:10.1016/j.jphotobiol.2020.111810

Cited by 41 publications

(38 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These data confirmed the potent antioxidant activity of MeDHICA-melanin [42] also in a cellular model, further highlighting its potential as an active ingredient in cosmetic formulations when compared to other natural or synthetic materials, such as phenol-rich plant extracts or other melanin-related samples. As an example, 10-fold higher concentrations (100 µg/mL) and longer pre-incubation times (1 h) have been reported in the case of a water extract from red grapevine leaves containing high levels of polyphenols to observe an effect comparable to that of the present study on the decrease of ROS generation in HaCaT cells irradiated with lower doses of UVA (25 J/cm 2 ) [38]. Also, the activity of silymarin was much lower than that observed with MeDHICA-melanin: 30 min of pre-incubation with 250 µg/mL of the compound were able to reduce the ROS produced by irradiating HaCaT cells with 20 J/cm 2 UVA by only 30% [50].…”

Section: Induction Of Nrf-2 Nuclear Translocation By Medhica-melaninsupporting

confidence: 72%

“…UV radiations are known to be very harmful for the human skin, as they induce reactive oxygen species (ROS) production. Both Ultraviolet A (UVA) and UVB are able to induce DNA damages [37,38]. UVB radiations induce DNA dimerization reactions between adjacent pyrimidine bases, whereas UVA radiations, weakly absorbed by DNA, can excite endogenous chromophores, leading to mispairing of DNA bases with consequent translation of mutated proteins [39].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications

Liberti¹,

Alfieri²,

Monti³

et al. 2020

Antioxidants

View full text Add to dashboard Cite

Eumelanins, the dark variant of skin pigments, are endowed with a remarkable antioxidant activity and well-recognized photoprotective properties that have been ascribed to pigment components derived from the biosynthetic precursor 5,6-dihydroxyindole-2-carboxylic acid (DHICA). Herein, we report the protective effect of a polymer obtained starting from the methyl ester of DHICA (MeDHICA-melanin) against Ultraviolet A (UVA)-induced oxidative stress in immortalized human keratinocytes (HaCaT). MeDHICA-melanin was prepared by aerial oxidation of MeDHICA. At concentrations as low as 10 µg/mL, MeDHICA-melanin prevented reactive oxygen species accumulation and partially reduced glutathione oxidation in UVA-irradiated keratinocytes. Western blot experiments revealed that the polymer is able to induce the translocation of nuclear factor erythroid 2–related factor 2 (Nrf-2) to the nucleus with the activation of the transcription of antioxidant enzymes, such as heme-oxygenase 1. Spectrophotometric and HPLC analysis of cell lysate allowed to conclude that a significant fraction (ca. 7%), consisting mainly of the 4,4′-dimer of MeDHICA (ca. 2 μM), was internalized in the cells. Overall these data point to the potential use of MeDHICA-melanin as an antioxidant for the treatment of skin damage, photoaging and skin cancers.

show abstract

Section: Induction Of Nrf-2 Nuclear Translocation By Medhica-melaninsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications

Liberti¹,

Alfieri²,

Monti³

et al. 2020

Antioxidants

View full text Add to dashboard Cite

show abstract

“…In addition to the decrease in ROS, the aqueous extract of tendrils has been shown to increase in a dose-and time-dependent manner the reduced glutathione concentrations in cultured human keratinocytes, thus increasing the main cellular antioxidant defense mechanism [38]. In a recent article, Marabini et al reported that the grapevine LE at a concentration of 100 µg/mL had a DNA-protective effect against UV-A and UV-B radiation in HaCaT cells by mitigating the induced oxidative stress [57]. The current findings are supported by the results of non-cellular assays, DPPH and FRAP, obtained within the present study, but also by other reports of the antioxidant potential of extracts from different parts of the V. vinifera in non-cellular assays such as FRAP, DPPH, and TEAC [58][59][60][61].…”

Section: Antioxidant Potentialmentioning

confidence: 99%

“…A randomized, double-blind, crossover clinical study using elite athletes reported that consumption of a grape extract standardized in flavanols permitted an improvement of the oxidative stress/antioxidant status balance during a competition period [65]. a DNA-protective effect against UV-A and UV-B radiation in HaCaT cells by mitigating the induced oxidative stress [57]. The current findings are supported by the results of non-cellular assays, DPPH and FRAP, obtained within the present study, but also by other reports of the antioxidant potential of extracts from different parts of the V. vinifera in non-cellular assays such as FRAP, DPPH, and TEAC [58][59][60][61].…”

Section: Antioxidant Potentialmentioning

confidence: 99%

Phytochemical Profile and Biological Activities of Tendrils and Leaves Extracts from a Variety of Vitis vinifera L.

et al. 2020

View full text Add to dashboard Cite

Winery industry by-products have a great reuse potential in the pharmaceutical and cosmetic fields due to their bioactive compounds. This study investigates the phytochemical profile and the bioactivity of Vitis vinifera variety Fetească neagră tendrils extract (TE) and leaves extract (LE), intended to be used in oral hygiene products recommended in periodontal disease. The evaluation of the phenolic content was performed by colorimetric analysis. Liquid chromatography coupled with mass spectrometry was used to determine the chemical profile of the two extracts obtained from V. vinifera. Moreover, the antioxidant activity of the extracts was determined by spectrophotometric methods, as well as on human gingival fibroblasts (HGF) cell line. The cytocompatibility and cytoprotective effect against nicotine-induced cytotoxicity were tested, as well as the anti-inflammatory and antimicrobial effects. The TE showed higher total polyphenolic content, rich in rutin, compared to the leaves extract that displayed important amounts of isoquercitrin. The antioxidant effect was confirmed by both non-cellular and cellular tests. The cytocompatibility of the extracts was confirmed at a wide range of concentrations. The cytoprotective effect was demonstrated in HGF exposed to cytotoxic doses of nicotine; 300 µg/mL of both tested extracts decreased nicotine toxicity by approximately 20%. When challenged with E. coli polysaccharides, in HGF cells co-exposed to TE and LE, a reduction in the release of proinflammatory cytokines (IL-8, IL-6 and IL-1β) was observed. The extracts were both able to reduce the levels of reactive oxygen species and inflammatory cytokines, and had notable antimicrobial effects on pathogenic bacteria associated with periodontitis.

show abstract

“…Polyphenol-rich extracts from Rubus spp. and grapevine (Vitis vinifera L.) exhibit pro-apoptotic effect after UVR exposure in vitro and in vivo, as reported in the literature [66][67][68]. More studies are needed to clarify the mechanism of apoptosis' induction from the extract pre-treatment along with UV-A induced damage.…”

Section: Discussionmentioning

confidence: 98%

Rhus coriaria L. Fruit Extract Prevents UV-A-Induced Genotoxicity and Oxidative Injury in Human Microvascular Endothelial Cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

Rhus coriaria L. (sumac) is a small plant widely diffused in the Mediterranean region. Its fruit are often consumed as a spice but are also present in traditional medicine of several countries. Recently, interest in this plant has increased and many scientific works reported its beneficial effects including antioxidant and anti-inflammatory properties. Plant extracts can be successfully used against ultraviolet rays, which are able to reach and damage the human skin; however, sumac extracts were never applied to this usage. Thus, in this study, we used a macerated ethanol extract of Rhus coriaria L. dried fruit (mERC) to demonstrate its preventive role against the damage induced by ultraviolet-A rays (UV-A) on microvascular endothelial cells (HMEC-1). In vitro effects of the extract pre-treatment and UV-A exposure were evaluated in detail. The antioxidant capacity was assessed by reactive oxygen species (ROS) formation and cellular antioxidant activity measurement. Genoprotective effects of mERC were investigated as well. Our findings indicate that the extract acts as a cell cycle inhibitor or apoptosis inducer, according to the level of damage. The present work provides new insights into the usage of Rhus coriaria extracts against skin injuries.

show abstract

Effects of Vitis vinifera L. leaves extract on UV radiation damage in human keratinocytes (HaCaT)

Cited by 41 publications

References 32 publications

A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications

A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications

Phytochemical Profile and Biological Activities of Tendrils and Leaves Extracts from a Variety of Vitis vinifera L.

Rhus coriaria L. Fruit Extract Prevents UV-A-Induced Genotoxicity and Oxidative Injury in Human Microvascular Endothelial Cells

Contact Info

Product

Resources

About