Effects of Vitamins C and E on Free Radical Processes in the Blood of Rats in Acute Moderate Hypothermia

Klichkhanov, N. K.; Ismailova, Zh. G.; Astaeva, M. D.; Chalabov, Shamil Ismailovich

doi:10.1134/s1062359019060086

Cited by 2 publications

(1 citation statement)

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“…Administration of vitamin C not only prevents an increase in the content of MDA in blood plasma under hypothermia of 30°C, but also contributes to a significant decrease in its level relative to the control. In addition, in RBCs, the administration of vitamin C significantly increased the GSH content in the cell during hypothermia (Klichkhanov et al, 2019). Meanwhile, Vitamin C was unable to completely correct the decrease of GSH as compared with control samples.…”

Section: Discussionmentioning

confidence: 99%

In vetro study on the toxic effects of acrylamide on blood antioxidants, ameliorative effects of vitamin C

Ghamdi

Alenezi

Algoferi

et al. 2020

Preprint

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Background: Acrylamide (ACR) is a naturally occurring, widely used compound, it is generated during cocking carbohydrate rich food at high temperature. Ingestion of large amounts of ACR underlies several health concerns and teratogenicity. Ascorbic acid (vit C) is a strong reducing agent greatly used to clean free radicals. Materials and methods:Blood sample was obtained from 46 years old, healthy nonsmoking man in heparinized tubs. Blood sample was immediately divided into seven parts as triplet for each. The first one was leaved as control, 2nd, 3rd and the 4th were treated with acrylamide in a concentration of 25,50 and 100 mM respectively, the 5th, 6th and the 7th were treated with acrylamide as the mentioned concentrations and vitamin C in a concentration of 100mM.Samples (one mile litter) from each tube were taken after four and 24 hours and were used for preparation of hemolysates, that were kept at -80°C till investigation of the biochemical parameters. Results: The concentrations of Malondialdehyd (MDA), nitric oxide (NO) and hydrogen peroxide (H2O2) increased in ACR and/or vit. C treated samples as compared with control. The concentration of reduced glutathione (GSH) and activities of Catalase (CAT), Superoxide dismutase (SOD), Glutathione reductase (GR), Glutathione peroxidase (GPx) and glucose 6 phosphate dehydrogenase (G6PDH) decreased significantly in ACR and/or vit. C treated samples as compared with control. Meanwhile, The concentrations of MDA, NO and H2O2 decreased in samples treated with both ACR and vit. C as compared with that treated with ACR only. The concentration of GSH and activities CAT, SOD, GR, GPx and G6PDH increased significantly in samples treated with both ACR and vit. C as compared with that treated with ACR only.Conclusion: ACR produce it's toxic effect through it's deleterious action on the antioxidant system through induction of pro-oxidants leading to exhausting of antioxidants. Vitamin C has an ameliorative action on the deleterious action exerted by ACR through improving the balance between pro-oxidants and antioxidant.

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Section: Discussionmentioning

confidence: 99%