In vetro study on the toxic effects of acrylamide on blood antioxidants, ameliorative effects of vitamin C

Abstract

Background: Acrylamide (ACR) is a naturally occurring, widely used compound, it is generated during cocking carbohydrate rich food at high temperature. Ingestion of large amounts of ACR underlies several health concerns and teratogenicity. Ascorbic acid (vit C) is a strong reducing agent greatly used to clean free radicals. Materials and methods:Blood sample was obtained from 46 years old, healthy nonsmoking man in heparinized tubs. Blood sample was immediately divided into seven parts as triplet for each. The first one was leaved as control, 2nd, 3rd and the 4th were treated with acrylamide in a concentration of 25,50 and 100 mM respectively, the 5th, 6th and the 7th were treated with acrylamide as the mentioned concentrations and vitamin C in a concentration of 100mM.Samples (one mile litter) from each tube were taken after four and 24 hours and were used for preparation of hemolysates, that were kept at -80°C till investigation of the biochemical parameters. Results: The concentrations of Malondialdehyd (MDA), nitric oxide (NO) and hydrogen peroxide (H2O2) increased in ACR and/or vit. C treated samples as compared with control. The concentration of reduced glutathione (GSH) and activities of Catalase (CAT), Superoxide dismutase (SOD), Glutathione reductase (GR), Glutathione peroxidase (GPx) and glucose 6 phosphate dehydrogenase (G6PDH) decreased significantly in ACR and/or vit. C treated samples as compared with control. Meanwhile, The concentrations of MDA, NO and H2O2 decreased in samples treated with both ACR and vit. C as compared with that treated with ACR only. The concentration of GSH and activities CAT, SOD, GR, GPx and G6PDH increased significantly in samples treated with both ACR and vit. C as compared with that treated with ACR only.Conclusion: ACR produce it's toxic effect through it's deleterious action on the antioxidant system through induction of pro-oxidants leading to exhausting of antioxidants. Vitamin C has an ameliorative action on the deleterious action exerted by ACR through improving the balance between pro-oxidants and antioxidant.

The impact of dietary nitrates and acrylamide intake on systemic redox status in healthy young adults

“…Upregulation of GPx, an important antioxidant enzyme, may indicate attenuated antioxidative response in subjects with the highest consumption of acrylamide [52]. Ghamdi et al [53] also showed a significant decrease in the concentration of GPx in healthy men after acrylamide ingestion, but authors also revealed GR depletion, which was not observed in the authors' study group [53]. However, the authors also noticed an students, but the authors did not observe any significant differences in subgroups divided by BMI value.…”

“…According to Zhao et al [53] GSH depletion is indicated as the final consequence of increased acrylamide exposure, since glycidamide can be conjugated to GSH as a detoxifying pathway of acrylamide. In general, most available literature data indicate OS induction by acrylamide, which is characterized by intensive lipid peroxidation (reflected by 8-iso-prostaglandin-F2α elevation), oxidative DNA damage (8-hydroxy-deoxyguanosine elevation), increased levels of protein-carbonyl as well as a decreased intensity of enzymatic and non-enzymatic antioxidant processes [56].…”