Effects of vitamin D binding protein-macrophage activating factor (DBP-MAF) infusion on bone resorption in two osteopetrotic mutations

Schneider, G; Benis, K.A.; Flay, Nina W.; Ireland, R.A.J.; Popoff, Steven N.

doi:10.1016/8756-3282(95)00118-w

Cited by 58 publications

(43 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of a GH-induced effect, one can hypothesize that high VDBP and low free vitamin D may play a role in predisposing to skeletal fragility, in agreement with the experimental evidence that VDBP is an inhibitor of vitamin D activity [30] and the clinical finding of association between high VDBP and low BMD in general population [8]. Moreover, it was also observed that VDBP may directly stimulate osteoclastogenesis and bone resorption in experimental conditions [31]. Future studies are, however, needed to test and confirm this hypothesis.…”

supporting

confidence: 66%

Vitamin D-binding protein: one more piece in the puzzle of acromegalic osteopathy?

2016

View full text Add to dashboard Cite

In only 5 years, more than 20,000 papers (more than 300 in the last month) dealing with vitamin D have been published, reflecting the deep interest of the scientific community for the role of this hormone in health and disease. There is an agreement that adequate vitamin D status should be defined by concentrations of serum that is the immediate precursor of the active hormone 1,25 dihydroxy-vitamin D3 [7]. However, in some conditions such parameter may not represent a reliable marker of vitamin D activity due to modifications of free hormone levels independently of total hormone storage and amount [8][9][10]. Consistently with the free hormone hypothesis, only hormone not bound to protein vectors can exert biological actions [11]. More than 80 % of circulating vitamin D (i.e., both 25(OH)-vitamin D and 1,25-dihydroxy-vitamin

show abstract

supporting

confidence: 66%

Vitamin D-binding protein: one more piece in the puzzle of acromegalic osteopathy?

2016

View full text Add to dashboard Cite

show abstract

“…In addition to being a transport protein, DBP is suggested to have a role in bone formation. In its deglycosylated form, DBP can act as a macrophage activating factor and produce morphological changes in osteoclasts and also activate them, which is correlated with enhanced bone resorption [2][3][4]. The DBP coding gene, GC gene, has two well-known missense variations in exon 11: a G > A substitution, which results in p.T416K, and a C > A substitution, which results in T420K, leading to a Threonine/Lysine amino acid change [5].…”

Section: Introductionmentioning

confidence: 99%

Serum parathyroid hormone is related to genetic variation in vitamin D binding protein with respect to total, free, and bioavailable 25-hydroxyvitamin D in middle-aged Caucasians – a cross-sectional study

et al. 2016

View full text Add to dashboard Cite

“…GalNAc-DBP complexed with oleic acid can be considered a physiologic/natural compound that is produced in mammal plasma during immune responses [17] and in milk and colostrum during fermentation [18,19]. GalNAc-DBP, as an isolated protein, has been studied in a number of experimental models of diseases, from osteopetrosis [17] to cancer [10] and autism [20].…”

Section: Discussionmentioning

confidence: 99%

“…GalNAc-DBP, as an isolated protein, has been studied in a number of experimental models of diseases, from osteopetrosis [17] to cancer [10] and autism [20].…”

Section: Discussionmentioning

confidence: 99%

Effect of glycosylated vitamin D binding protein complexed with oleic acid on human myeloma and Hodgkin’s lymphoma cultures

Branca¹,

Smith²,

Ward³

et al. 2015

Integr Cancer Sci Therap

View full text Add to dashboard Cite

N-Acetylgalactosamine-glycosylated vitamin D binding protein (GalNAc-DBP) is known to stimulate macrophages and to directly inhibit angiogenesis and cancer cell growth in vitro. In this study, we evaluated the direct effect of GalNAc-DBP complexed with oleic acid on human multiple myeloma and Hodgkin's lymphoma cell lines (KMS-12-BM and L540 respectively) as well as the effect, on the same cell lines, of human spleen macrophages (CRL9853) previously activated by GalNAc-DBP complexed with oleic acid.Cell viability and living cell number were evaluated respectively by Tetrazolium dye cell viability assay and by Trypan Blue staining. Interactions between activated macrophages and cancer cells were studied by time lapse photography. Our results show that GalNAc-DBP complexed with oleic acid inhibits the proliferation of myeloma and Hodgkin's lymphoma cells in a dose-dependent manner. Furthermore, GalNAc-DBP complexed with oleic acid activates human spleen macrophages which in turn phagocytise cancer cells. In addition, we observed that GalNAc-DBP complexed with oleic acid activates human spleen macrophages in vivo as documented by color-doppler ultrasonography of the spleen. These results demonstrate that GalNAc-DBP complexed with oleic acid has a double effect on myeloma and Hodgkin's lymphoma cancer cells: a direct inhibition of their proliferation and viability and, at the same time, an efficient macrophage activation leading to a significant depletion of cancer cell population. Taken together, these results suggest that GalNAc-DBP complexed with oleic acid may prove effective in the integrative immunotherapy of multiple myeloma and Hodgkin's lymphoma.

show abstract

Effects of vitamin D binding protein-macrophage activating factor (DBP-MAF) infusion on bone resorption in two osteopetrotic mutations

Cited by 58 publications

References 27 publications

Vitamin D-binding protein: one more piece in the puzzle of acromegalic osteopathy?

Vitamin D-binding protein: one more piece in the puzzle of acromegalic osteopathy?

Serum parathyroid hormone is related to genetic variation in vitamin D binding protein with respect to total, free, and bioavailable 25-hydroxyvitamin D in middle-aged Caucasians – a cross-sectional study

Effect of glycosylated vitamin D binding protein complexed with oleic acid on human myeloma and Hodgkin’s lymphoma cultures

Contact Info

Product

Resources

About