Effects of Vitamin C on the Prevention of Ischemia-Reperfusion Brain Injury: Experimental Study in Rats

Abstract

Background. Reperfusion syndrome after carotid endarterectomy is a complication associated with cerebrovascular self-regulation in a chronically hypoperfused cerebral hemisphere, leading to severe neurological damage. Vitamin C is an important antioxidant in brain metabolism that has shown some neuroprotective actions. Objective. To investigate the potential effects of vitamin C on cerebral reperfusion in comparison with placebo (saline) in rats. Methods. Male Wistar rats were divided into 3 groups: (i) Sham (n=4), animals exposed to carotid arteries dissection without clamping; (ii) Control (n=4), animals that received an intraperitoneal injection of 0.9% saline solution (0.1 mL/kg) and underwent carotid arteries dissection with temporary clamping; (iii) Vitamin C (n=4), animals that received an intraperitoneal injection of vitamin C (750 mg/kg) and underwent carotid arteries dissection with temporary clamping. Behavioral assessment was then performed in all groups, which included the open field, Morris water maze and rotarod tests. Levels of malondialdehyde (MDA) in the hippocampus and striatum were measured using a fluorometric assay. Results. Rats treated with vitamin C presented with a similar behavior as compared to the Sham group in all the three tests (p>0.05), but it was significantly different from controls (p<0.05). Vitamin C was also found to reduce MDA levels in both hippocampus and striatum when compared to placebo (p<0.05). Conclusion. In the present study, vitamin C was associated with behavioral and motor preservation as well as decreased cerebral MDA levels after induced cerebral ischemia in rats.

Study on the Influencing Factors of Osteoarthritis in Southern China

“…Studies by Das Gupta et al [28,29] reported that alcohol consumption is a risk factor for OA, so in daily life, it is recommended to drink less alcohol and eat more fresh vegetables, fruits, and soy products. Studies by McAlindon et al [30,31] reported that prolonged sunlight exposure is a protective factor for OA, because vitamin D has the activity of regulating bone metabolism in the body, it is mainly synthesized in the skin through sunlight, and prolonged sun exposure results in high levels of active vitamin D synthesized in the body, and active vitamin D can promote bone growth and metabolism, which protects the body from OA. e results of this study suggest that body mass index increases the risk of OA.…”

Novel Combination of COX-2 Inhibitor and Antioxidant Therapy for Modulating Oxidative Stress Associated with Intestinal Ischemic Reperfusion Injury and Endotoxemia

“…Rats were randomly divided into the following groups ( n = 10): Firocoxib (10 mg/kg), vitamin C (750 mg/kg) alone, or firocoxib (10 mg/kg) in combination with vitamin C (750 mg/kg), administered intraperitoneally 30 min before I/R induction [ 19 , 20 , 21 , 22 ]. Animals were anesthetized with sodium pentobarbital (45 mg/kg) and the induction of I/R was performed as previously described [ 23 ].…”

Neuroprotective and Anti-Inflammatory Activities of Hybrid Small-Molecule SA-10 in Ischemia/Reperfusion-Induced Retinal Neuronal Injury Models

“…Hence, there remains an unmet need to identify potential therapeutic agents that can address the intricate pathophysiology of ischemia/reperfusion retinal injury. Notably, antioxidants, including vitamins C and E, have shown promising therapeutic benefits in retinal ischemic injury and rodent stroke models [ 9 , 10 , 11 ]. Additionally, free radical scavengers, like NXY-059 (disodium 2,4-sulphophenyl- N -tert-butylnitrone, Cerovive) and thiol-containing N -acetyl cysteine, have proven effective in reducing the infarct size and improving neurological outcomes in brain stroke models [ 12 , 13 ].…”