Effects of Visfatin (PBEF/NAMPT) on Feeding and Hypothalamic Peptide Gene Expression in the Rat

Brunetti, Luigi; Lucia, Recinella; Nisio, Chiara Di; Chiavaroli, Annalisa; Orlando, Giustino; Ferrante, Claudio; Leone, Sheila; Vacca, Michèle

doi:10.1210/endo-meetings.2011.part2.p34.p2-295

Cited by 14 publications

(31 citation statements)

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“…101 A role for NAMPT in the regulation of food intake and behaviour was demonstrated in two different animal models. 102,103 Intracerebroventricular injection of eNAMPT caused increased feed intake and pecking efficiency in chicks, 102 whereas injection of eNAMPT into the arcuate nucleus of the hypothalamus of rats increased food intake and reduced hypothalamic expression of anorexigenic peptides. 103 A 2015 study indicated that eNAMPT secreted by adipose tissue influences hypothalamic function.…”

Section: Pathophysiological Role Of Namptmentioning

confidence: 99%

“…102,103 Intracerebroventricular injection of eNAMPT caused increased feed intake and pecking efficiency in chicks, 102 whereas injection of eNAMPT into the arcuate nucleus of the hypothalamus of rats increased food intake and reduced hypothalamic expression of anorexigenic peptides. 103 A 2015 study indicated that eNAMPT secreted by adipose tissue influences hypothalamic function. 55 In mice with an adipose-tissue-specific knockout of NAMPT (designated ANKO), levels of eNAMPT were notably reduced.…”

Section: Pathophysiological Role Of Namptmentioning

confidence: 99%

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Physiological and pathophysiological roles of NAMPT and NAD metabolism

et al. 2015

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Nicotinamide phosphoribosyltransferase (NAMPT) is a regulator of the intracellular nicotinamide adenine dinucleotide (NAD) pool. NAD is an essential coenzyme involved in cellular redox reactions and is a substrate for NAD-dependent enzymes. In various metabolic disorders and during ageing, levels of NAD are decreased. Through its NAD-biosynthetic activity, NAMPT influences the activity of NAD-dependent enzymes, thereby regulating cellular metabolism. In addition to its enzymatic function, extracellular NAMPT (eNAMPT) has cytokine-like activity. Abnormal levels of eNAMPT are associated with various metabolic disorders. NAMPT is able to modulate processes involved in the pathogenesis of obesity and related disorders such as nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM) by influencing the oxidative stress response, apoptosis, lipid and glucose metabolism, inflammation and insulin resistance. NAMPT also has a crucial role in cancer cell metabolism, is often overexpressed in tumour tissues and is an experimental target for antitumour therapies. In this Review, we discuss current understanding of the functions of NAMPT and highlight progress made in identifying the physiological role of NAMPT and its relevance in various human diseases and conditions, such as obesity, NAFLD, T2DM, cancer and ageing.

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Section: Pathophysiological Role Of Namptmentioning

confidence: 99%

Section: Pathophysiological Role Of Namptmentioning

confidence: 99%

Physiological and pathophysiological roles of NAMPT and NAD metabolism

et al. 2015

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“…The intracellular form (iNAMPT) is acetylated, and under fasted conditions, SIRT1-mediated lysine deacetylation leads to its secretion as an extracellular enzyme (eNAMPT) [75]. eNAMPT contributes significantly to the circulating NMN pool, providing building blocks for NAD + synthesis in pancreatic β-cells to support insulin secretion [55] and in the hypothalamus to increase locomotor activity [75] and food intake [7].…”

Section: Enampt (Visfatin or Pbef)mentioning

confidence: 99%

Adipose crosstalk with other cell types in health and disease

Choi

Cohen

2017

Experimental Cell Research

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“…Despite the growing body of evidence supporting the important role of SIRT1 in food intake, few studies have explored the contribution of NAMPT and the NAD + salvage pathway in this process. Stereotaxic administration of NAMPT in the Arc of rats resulted in increased food intake and decreased dopamine and Cart mRNA levels . Recently, NAD + ICV administration was shown to reduce fasting‐induced food intake possibly via astrocytic connexin 43 transporter .…”

Section: Introductionmentioning

confidence: 99%

Fasting‐ and ghrelin‐induced food intake is regulated by NAMPT in the hypothalamus

et al. 2020

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Aim Neurons in the arcuate nucleus of the hypothalamus are involved in regulation of food intake and energy expenditure, and dysregulation of signalling in these neurons promotes development of obesity. The role of the rate‐limiting enzyme in the NAD+ salvage pathway, nicotinamide phosphoribosyltransferase (NAMPT), for regulation energy homeostasis by the hypothalamus has not been extensively studied. Methods We determined whether Nampt mRNA or protein levels in the hypothalamus of mice were affected by diet‐induced obesity, by fasting and re‐feeding, and by leptin and ghrelin treatment. Primary hypothalamic neurons were treated with FK866, a selective inhibitor of NAMPT, or rAAV carrying shRNA directed against Nampt, and levels of reactive oxygen species (ROS) and mitochondrial respiration were assessed. Fasting and ghrelin‐induced food intake was measured in mice in metabolic cages after intracerebroventricular (ICV)‐mediated FK866 administration. Results NAMPT levels in the hypothalamus were elevated by administration of ghrelin and leptin. In diet‐induced obese mice, both protein and mRNA levels of NAMPT decreased in the hypothalamus. NAMPT inhibition in primary hypothalamic neurons significantly reduced levels of NAD+, increased levels of ROS, and affected the expression of Agrp, Pomc and genes related to mitochondrial function. Finally, ICV‐induced NAMPT inhibition by FK866 did not cause malaise or anhedonia, but completely ablated fasting‐ and ghrelin‐induced increases in food intake. Conclusion Our findings indicate that regulation of NAMPT levels in hypothalamic neurons is important for the control of fasting‐ and ghrelin‐induced food intake.

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Effects of Visfatin (PBEF/NAMPT) on Feeding and Hypothalamic Peptide Gene Expression in the Rat

Cited by 14 publications

References 0 publications

Physiological and pathophysiological roles of NAMPT and NAD metabolism

Physiological and pathophysiological roles of NAMPT and NAD metabolism

Adipose crosstalk with other cell types in health and disease

Fasting‐ and ghrelin‐induced food intake is regulated by NAMPT in the hypothalamus

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